Mouse/Rat NCAM-1/CD56 Antibody

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Detection of Rat NCAM‑1/CD56 by Western Blot.
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Product Details
Citations (1)
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Mouse/Rat NCAM-1/CD56 Antibody Summary

Species Reactivity
Mouse, Rat
Detects mouse and rat NCAM-1/CD56 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human NCAM-1 is observed.  
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse NCAM-1/CD56
Leu20-Thr711 (Phe403Val)
Accession # P13595
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat NCAM-1/CD56 antibody by Western Blot. View Larger

Detection of Rat NCAM‑1/CD56 by Western Blot. Western blot shows lysates of rat hippocampal embryonic neurons. PVDF Membrane was probed with 1 µg/mL of Mouse NCAM-1/CD56 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6070) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NCAM-1/CD56 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NCAM-1/CD56

NCAM-1 (Neural adhesion molecule-1; also CD56) is a 120 -190 kDa glycoprotein member of the Ig Superfamily. It is expressed on multiple cell types, both in the embryo and adult. Here, it serves as both an adhesion molecule and a receptor for multiple ligands, including as FGFR, PDGF, GDNF and agrin. On the cell surface, it is a cis-oriented homodimer that can form homodimers in-trans with other cis-homodimers. In the embryo, NCAM-1 is polysialylated (PolySia), and shows a MW of 200-220 kDa in SDS-Page. This polysialylation reduces the ability of NCAM-1 to dimerize. Mature mouse NCAM-1 is a 1096 amino acid (aa) type I transmembrane (TM) protein (aa 20-1115). It possesses a 692 aa extracellular region (aa 20-711) and a 386 aa cytoplasmic domain. The extracellular region contains five consecutive C2-type Ig-like domains (aa 20-492) followed by two FN type-III domains (aa 497-692). Multiple splice variants exist. There is a 140 kDa TM variant that shows a deletion of aa 810-1076, and a 120 kDa variant that is GPI-linked and shows a 24 aa substitution for aa 702-1115. A third potential variant contains a five aa substitution for aa 601-1115. Over aa 20-711, mouse NCAM-1 shares 99% and 95% aa identity with rat and human NCAM-1, respectively.

Long Name
Neural Cell Adhesion Molecule
Entrez Gene IDs
4684 (Human); 17967 (Mouse); 24586 (Rat)
Alternate Names
CD56 / NCAM-1; CD56 antigen; CD56; MSK39; NCAM1; N-CAM-1; NCAM-1; NCAMantigen recognized by monoclonal 5.1H11; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM

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Citation for Mouse/Rat NCAM-1/CD56 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Seizure protein 6 controls glycosylation and trafficking of kainate receptor subunits GluK2 and GluK3
    Authors: Martina Pigoni, Hung‐En Hsia, Jana Hartmann, Jasenka Rudan Njavro, Merav D Shmueli, Stephan A Müller et al.
    The EMBO Journal


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