NCAM-1/CD56 Antibody

Name: NCAM-1/CD56 Antibody
Brand: R&D Systems
SKU: AF6070
Price: 159 USD
Availability: InStock

R&D Systems | Catalog # AF6070

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all NCAM-1/CD56 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse NCAM-1/CD56
Leu20-Thr711 (Phe403Val)
Accession # P13595

Specificity

Detects mouse and rat NCAM-1/CD56 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human NCAM-1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for NCAM-1/CD56 Antibody

Detection of Rat NCAM‑1/CD56 by Western Blot.

Western blot shows lysates of rat hippocampal embryonic neurons. PVDF Membrane was probed with 1 µg/mL of Mouse NCAM-1/CD56 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6070) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NCAM-1/CD56 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for NCAM-1/CD56 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Rat hippocampal embryonic neurons

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: NCAM-1/CD56

NCAM-1 (Neural adhesion molecule-1; also CD56) is a 120 -190 kDa glycoprotein member of the Ig Superfamily. It is expressed on multiple cell types, both in the embryo and adult. Here, it serves as both an adhesion molecule and a receptor for multiple ligands, including as FGFR, PDGF, GDNF and agrin. On the cell surface, it is a cis-oriented homodimer that can form homodimers in-trans with other cis-homodimers. In the embryo, NCAM-1 is polysialylated (PolySia), and shows a MW of 200-220 kDa in SDS-Page. This polysialylation reduces the ability of NCAM-1 to dimerize. Mature mouse NCAM-1 is a 1096 amino acid (aa) type I transmembrane (TM) protein (aa 20-1115). It possesses a 692 aa extracellular region (aa 20-711) and a 386 aa cytoplasmic domain. The extracellular region contains five consecutive C2-type Ig-like domains (aa 20-492) followed by two FN type-III domains (aa 497-692). Multiple splice variants exist. There is a 140 kDa TM variant that shows a deletion of aa 810-1076, and a 120 kDa variant that is GPI-linked and shows a 24 aa substitution for aa 702-1115. A third potential variant contains a five aa substitution for aa 601-1115. Over aa 20-711, mouse NCAM-1 shares 99% and 95% aa identity with rat and human NCAM-1, respectively.