Mouse/Rat Prolactin Antibody

Catalog # Availability Size / Price Qty
AF1445
AF1445-SP
Mouse/Rat Prolactin Antibody in Western Blot
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Product Details
Citations (5)
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Mouse/Rat Prolactin Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Prolactin in Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human Prolactin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Prolactin
Leu32-Cys228
Accession # NP_035294
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse skin and thymus

Mouse Prolactin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Prolactin Biotinylated Antibody (Catalog # BAF1445)

Standard: Recombinant Mouse Prolactin Protein (Catalog # 1445-PL)

Neutralization
Measured by its ability to neutralize Prolactin-induced proliferation in the Nb2‑11 rat lymphoma cell line. Gout, P. W. et al. (1980) Cancer Research 40:2433. The Neutralization Dose (ND50) is typically 0.25-1.0 µg/mL in the presence of 10 ng/mL Recombinant Mouse Prolactin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse and Rat Prolactin by Western Blot. View Larger

Detection of Mouse and Rat Prolactin by Western Blot. Western blot shows lysates of mouse pituitary tissue and rat pituitary tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization Cell Proliferation Induced by Prolactin and Neutralization by Mouse Prolactin Antibody. View Larger

Cell Proliferation Induced by Prolactin and Neutralization by Mouse Prolactin Antibody. Recombinant Mouse Prolactin (Catalog # 1445-PL) stimulates proliferation in the Nb2-11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Rec-ombinant Mouse Prolactin (10 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse/Rat Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1445). The ND50is typically 0.25-1.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prolactin

Prolactin (PRL) is a neuroendocrine pituitary hormone. Prolactin is synthesized by the anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, NK cells and breast cancer cells. Originally characterized as a lactogenic hormone, further studies have demonstrated broader roles in breast cancer development, regulation of reproductive function, and immunoregulation. In the immune system, Prolactin has been shown to be secreted by human PBMC and to act as a proliferative growth factor. Additionally, Prolactin treatment of human PBMC has been shown to enhance IFN-gamma production. In the breast, Prolactin-induced morphogenesis of the mammary cells is mediated through IGF-2, which in turn upregulates cyclin D1. Prolactin has several molecular forms. The predominant form is a monomer; the non-glycosylated form is 23 kDa and the glycosylated form is 25 kDa. Glycosylated Prolactin is removed from the circulation faster and has been reported to have lower biological potency. Mouse Prolactin cDNA encodes a 228 amino acid (aa) residue protein with a putative 31 aa residue signal peptide. The Prolactin receptor is a transmembrane type I glycoprotein that belongs to the cytokine hematopoietic receptor family. B cells, T cells, macrophages, NK cells, monocytes, CD34+ progenitor cells, neutrophils, mammary gland, liver, kidney, adrenals, ovaries, testis, prostrate, seminal vesicles, and hypothalamus have all been shown to express the Prolactin receptor. Three forms of the receptor, generated by differential splicing, have been identified. These isoforms differ in the length of their cytoplasmic domains. It is believed that the short cytoplasmic form is non-functional. Prolactin signal transduction involves the JAK/STAT families and Src kinase family (1‑9).

References
  1. Freeman, M. et al. (2000) Physiological Reviews 80:1523.
  2. Ben-Johnson, N. et al. (1996) Endoc. Rev. 17:639.
  3. Cesario, T. et al. (1994) Proc. Soc. Exp. Biol. Med. 205:89.
  4. Price, A.E. et al. (1995) Endoc. 136:4827.
  5. Hoffmann, T. et al. (1993) J. Endoc. Invest. 16:807.
  6. Cole, E. et al. (1991) Endoc. 129:2639.
  7. Lewis, U. et al. (1985) Endoc. 116:359.
  8. Matalk, K. (2003) Cytokine 21:187.
  9. Brisken, C. et al. (2002) Dev. Cell 3:877.
Entrez Gene IDs
5617 (Human); 19109 (Mouse)
Alternate Names
PRL; Prolactin

Product Datasheets

Citations for Mouse/Rat Prolactin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Chemokine CXCL14-like immunoreactivity in the &alphaMSH-producing cells and PRL-producing cells of the flat-tailed house gecko pituitary
    Authors: H Suzuki, T Yamamoto
    J. Vet. Med. Sci., 2020;82(4):408-413.
    Species: Gecko
    Sample Types: Whole Tissue
    Applications: IF
  2. Bone loss caused by dopaminergic degeneration and levodopa treatment in Parkinson's disease model mice
    Authors: K Handa, S Kiyohara, T Yamakawa, K Ishikawa, M Hosonuma, N Sakai, A Karakawa, M Chatani, M Tsuji, K Inagaki, Y Kiuchi, M Takami, T Negishi-Ko
    Sci Rep, 2019;9(1):13768.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Completely humanizing prolactin rescues infertility in prolactin knockout mice and leads to human prolactin expression in extrapituitary mouse tissues.
    Authors: Christensen, Heather, Murawsky, Michael, Horseman, Nelson D, Willson, Tara A, Gregerson, Karen A
    Endocrinology, 2013;154(12):4777-89.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr
  4. Paternal recognition of adult offspring mediated by newly generated CNS neurons.
    Authors: Mak GK, Weiss S,
    Nat. Neurosci., 2010;13(6):753-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  5. Blocking vascular endothelial growth factor-a inhibits the growth of pituitary adenomas and lowers serum prolactin level in a mouse model of multiple endocrine neoplasia type 1.
    Authors: Korsisaari N, Ross J, Wu X, Kowanetz M, Pal N, Hall L, Eastham-Anderson J, Forrest WF, van Bruggen N, Peale FV, Ferrara N
    Clin. Cancer Res., 2008;14(1):249-58.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Secondary Antibodies

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