Mouse/Rat RAGE Antibody

Catalog # Availability Size / Price Qty
MAB1179-SP
MAB1179-100
MAB1179-500
Mouse/Rat RAGE Antibody in Western Blot
2 Images
Product Details
Citations (12)
FAQs
Supplemental Products
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Mouse/Rat RAGE Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat RAGE in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human RAGE is observed.
Source
Monoclonal Rat IgG2A Clone # 175410
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse RAGE
Gly23-Ala342
Accession # NP_031451
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse RAGE by Western Blot. View Larger

Detection of Mouse RAGE by Western Blot.
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat RAGE Monoclonal Antibody  (Catalog # 1179) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for RAGE and mature RAGE at approximately 50 and 38 kDa, respectively (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry RAGE in Mouse Lung. View Larger

RAGE in Mouse Lung. RAGE was detected in perfusion fixed frozen sections of adult mouse lung using Rat Anti-Mouse/Rat RAGE Monoclonal Antibody (Catalog # MAB1179) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Northern­Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RAGE/AGER

Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules (1). AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multiligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins (1, 2). The mouse RAGE gene encodes a 403 amino acid (aa) residue type I transmembrane glycoprotein with a 22 aa signal peptide, a 319 aa extracellular domain containing a Ig-like V‑type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain (3). The V‑type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V‑type domain or the cytoplasmic tail, are known (1, 4). RAGE is highly expressed in the embryonic central nervous system (5). In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes and hepatocytes (6). The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression (1, 7). RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses, and immune or inflammatory disorders (1).

References
  1. Schmidt, A. et al. (2001) J. Clin. Invest. 108:949.
  2. Chavakis, T. et al. (2003) J. Exp. Med. 198:507.
  3. Renard, C. et al. (1997) Mol. Pharmacol. 52:54.
  4. Yonekura, H. et al. (2003) Biochem. J. 370:1097.
  5. Hori, O. et al. (1995) J. Biol. Chem. 270:25752.
  6. Brett, J. et al. (1993) Am. J. Pathol. 143:1699.
  7. Valencia, J.V. et al. (2004) Diabetes 53:743.
Long Name
Receptor for Advanced Glycation End Products
Entrez Gene IDs
177 (Human); 11596 (Mouse); 81722 (Rat); 403168 (Canine)
Alternate Names
advanced glycosylation end product-specific receptor; AGER; RAGE isoform delta; RAGE isoform sRAGE-delta; RAGE; Receptor for advanced glycosylation end products; receptor for advanced glycosylation end-products; SCARJ1

Product Datasheets

Citations for Mouse/Rat RAGE Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. IL-1 and TNF? Contribute to the Inflammatory Niche to Enhance Alveolar Regeneration
    Authors: H Katsura, Y Kobayashi, PR Tata, BLM Hogan
    Stem Cell Reports, 2019;0(0):.
    Species: Mouse
    Sample Types: Organoids
    Applications: IHC-P
  2. RAGE-specific single chain Fv for PET imaging of pancreatic cancer
    Authors: HY Kim, X Wang, R Kang, D Tang, BA Boone, HJ Zeh, MT Lotze, WB Edwards
    PLoS ONE, 2018;13(3):e0192821.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Pulmonary pericytes regulate lung morphogenesis
    Authors: K Kato, R Diéguez-Hu, DY Park, SP Hong, S Kato-Azuma, S Adams, M Stehling, B Trappmann, JL Wrana, GY Koh, RH Adams
    Nat Commun, 2018;9(1):2448.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Therapeutic Effectiveness of Anti-RAGE Antibody Administration in a Rat Model of Crush Injury
    Authors: H Matsumoto, N Matsumoto, J Shimazaki, J Nakagawa, Y Imamura, K Yamakawa, T Yamada, M Ikeda, H Hiraike, H Ogura, T Shimazu
    Sci Rep, 2017;7(1):12255.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  5. Recruited Monocytes and Type 2 Immunity Promote Lung Regeneration following Pneumonectomy
    Authors: AJ Lechner, IH Driver, J Lee, CM Conroy, A Nagle, RM Locksley, JR Rock
    Cell Stem Cell, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. The development and plasticity of alveolar type 1 cells
    Authors: J Yang, BJ Hernandez, D Martinez A, O Narvaez de, L Vila-Ellis, H Akiyama, SE Evans, EJ Ostrin, J Chen
    Development, 2016;143(1):54-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. Alveolar progenitor and stem cells in lung development, renewal and cancer.
    Authors: Desai, Tushar J, Brownfield, Douglas, Krasnow, Mark A
    Nature, 2014;507(7491):190-4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Two nested developmental waves demarcate a compartment boundary in the mouse lung.
    Authors: Alanis, Denise M, Chang, Daniel R, Akiyama, Haruhiko, Krasnow, Mark A, Chen, Jichao
    Nat Commun, 2014;5(0):3923.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Receptor for advanced glycation end products (RAGE) functions as receptor for specific sulfated glycosaminoglycans, and anti-RAGE antibody or sulfated glycosaminoglycans delivered in vivo inhibit pulmonary metastasis of tumor cells.
    Authors: Mizumoto S, Takahashi J, Sugahara K
    J. Biol. Chem., 2012;287(23):18985-94.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. Aldose reductase and AGE-RAGE pathways: central roles in the pathogenesis of vascular dysfunction in aging rats.
    Authors: Hallam KM, Li Q, Ananthakrishnan R, Kalea A, Zou YS, Vedantham S, Schmidt AM, Yan SF, Ramasamy R
    Aging Cell, 2010;9(5):776-84.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. Role of soluble receptor for advanced glycation end products on endotoxin-induced lung injury.
    Authors: Zhang H, Tasaka S, Shiraishi Y, Fukunaga K, Yamada W, Seki H, Ogawa Y, Miyamoto K, Nakano Y, Hasegawa N, Miyasho T, Maruyama I, Ishizaka A
    Am. J. Respir. Crit. Care Med., 2008;178(4):356-62.
    Species: Mouse
    Sample Types: BALF
    Applications: Western Blot
  12. Alpha-4/beta-1 and alpha-L/beta-2 integrins mediate cytokine induced lung leukocyte-epithelial adhesion and injury.
    Authors: Parmley LA, Elkins ND, Fini MA, Liu YE, Repine JE, Wright RM
    Br. J. Pharmacol., 2007;152(6):915-29.
    Species: Rat
    Sample Types: BALF
    Applications: Western Blot

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