Mouse/Rat Serpin F1/PEDF Antibody

Catalog # Availability Size / Price Qty
AF1149
AF1149-SP
Mouse/Rat Serpin F1/PEDF Antibody in Western Blot
2 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Mouse/Rat Serpin F1/PEDF Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse Serpin F1/PEDF in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human Serpin F1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Serpin F1/PEDF
Asp43-Thr417
Accession # BAA31978
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
20 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse and Rat Serpin F1/PEDF by Western Blot. View Larger

Detection of Mouse and Rat Serpin F1/PEDF by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, 3T3-L1 mouse embryonic fibroblast adipose-like cell line, NRK rat normal kidney cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse/Rat Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1149) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Serpin F1/PEDF at approximately 46-48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Mouse Serpin F1/PEDF by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Serpin F1/PEDF by Simple WesternTM. Simple Western lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Serpin F1/PEDF at approximately 55 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse/Rat Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1149) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin F1/PEDF

Serpin F1 is a secreted glycoprotein that shares sequence and structural similarity with the serpin (serine proteinase inhibitor) superfamily proteins but lacks serine protease inhibitory activity.

Long Name
Serpine F1/Pigment Epithelium-derived Factor
Entrez Gene IDs
5176 (Human); 20317 (Mouse); 287526 (Rat)
Alternate Names
Cell proliferation-inducing gene 35 protein; EPC-1; EPC-1PIG35; PEDF; PEDFpigment epithelium-derived factor; pigment epithelium derived factor), member 1; proliferation-inducing protein 35; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin; Serpin F1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1

Product Datasheets

Citations for Mouse/Rat Serpin F1/PEDF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Systemic administration of erythropoietin inhibits retinopathy in RCS rats.
    Authors: Shen W, Chung S, Irhimeh M, Li S, Lee S, Gillies M
    PLoS ONE, 2014;9(8):e104759.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Adeno-associated virus vector-mediated delivery of pigment epithelium-derived factor restricts neuroblastoma angiogenesis and growth.
    Authors: Streck CJ, Zhang Y, Zhou J, Ng C, Nathwani AC, Davidoff AM
    J. Pediatr. Surg., 2005;40(1):236-43.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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