Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Serpin F1/PEDF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Serpin F1/PEDF
Asp43-Thr417
Accession # BAA31978

Specificity

Detects mouse Serpin F1/PEDF in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human Serpin F1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse/Rat Serpin F1/PEDF Antibody

Detection of Mouse and Rat Serpin F1/PEDF antibody by Western Blot.

Detection of Mouse and Rat Serpin F1/PEDF by Western Blot.

Western blot shows lysates of C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, 3T3-L1 mouse embryonic fibroblast adipose-like cell line, NRK rat normal kidney cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse/Rat Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1149) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Serpin F1/PEDF at approximately 46-48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse Serpin F1/PEDF antibody by Simple WesternTM.

Detection of Mouse Serpin F1/PEDF by Simple WesternTM.

Simple Western lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Serpin F1/PEDF at approximately 55 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse/Rat Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1149) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Serpin F1/PEDF by Western Blot

Detection of Serpin F1/PEDF by Western Blot

Western blot verification of selected muscle proteins commonly regulated by UND in both the PL and SOL muscles. (A,E) Representative western blot images and densitometric analysis of (B,F) beta -oxidation enzyme ACADM, (C,G) oxidative phosphorylation enzyme CYTC, and (D,H) glycoprotein SERPINF1 levels in PL and SOL muscles from UND vs. CTRL rats (n = 6/group). Results are expressed as means ± SEM. Unpaired student’s t-test was performed to test the effect of undernutrition (*, P < 0.05; **, P < 0.01). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35563153), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Serpin F1/PEDF by Western Blot

Detection of Serpin F1/PEDF by Western Blot

Western blot verification of selected muscle proteins commonly regulated by UND in both the PL and SOL muscles. (A,E) Representative western blot images and densitometric analysis of (B,F) beta -oxidation enzyme ACADM, (C,G) oxidative phosphorylation enzyme CYTC, and (D,H) glycoprotein SERPINF1 levels in PL and SOL muscles from UND vs. CTRL rats (n = 6/group). Results are expressed as means ± SEM. Unpaired student’s t-test was performed to test the effect of undernutrition (*, P < 0.05; **, P < 0.01). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35563153), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Serpin F1/PEDF by Western Blot

Detection of Serpin F1/PEDF by Western Blot

Western blot verification of selected muscle proteins commonly regulated by UND in both the PL and SOL muscles. (A,E) Representative western blot images and densitometric analysis of (B,F) beta -oxidation enzyme ACADM, (C,G) oxidative phosphorylation enzyme CYTC, and (D,H) glycoprotein SERPINF1 levels in PL and SOL muscles from UND vs. CTRL rats (n = 6/group). Results are expressed as means ± SEM. Unpaired student’s t-test was performed to test the effect of undernutrition (*, P < 0.05; **, P < 0.01). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35563153), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Serpin F1/PEDF by Western Blot

Detection of Serpin F1/PEDF by Western Blot

Western blot verification of selected muscle proteins commonly regulated by UND in both the PL and SOL muscles. (A,E) Representative western blot images and densitometric analysis of (B,F) beta -oxidation enzyme ACADM, (C,G) oxidative phosphorylation enzyme CYTC, and (D,H) glycoprotein SERPINF1 levels in PL and SOL muscles from UND vs. CTRL rats (n = 6/group). Results are expressed as means ± SEM. Unpaired student’s t-test was performed to test the effect of undernutrition (*, P < 0.05; **, P < 0.01). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35563153), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse/Rat Serpin F1/PEDF Antibody

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: NIH‑3T3 mouse embryonic fibroblast cell line

Western Blot

0.2 µg/mL
Sample: C2C12 mouse myoblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, NRK rat normal kidney cell line, and Rat‑2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin F1/PEDF

Serpin F1 is a secreted glycoprotein that shares sequence and structural similarity with the serpin (serine proteinase inhibitor) superfamily proteins but lacks serine protease inhibitory activity.

Long Name

Serpine F1/Pigment Epithelium-derived Factor

Alternate Names

EPC-1, PEDF

Entrez Gene IDs

5176 (Human); 20317 (Mouse); 287526 (Rat)

Gene Symbol

SERPINF1

UniProt

BAA31978

Additional Serpin F1/PEDF Products

Product Documents for Mouse/Rat Serpin F1/PEDF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat Serpin F1/PEDF Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Mouse/Rat Serpin F1/PEDF Antibody

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