Mouse/Rat Thioredoxin-like 5/TRP14 Antibody

Catalog # Availability Size / Price Qty
AF3769
AF3769-SP
Detection of Mouse/Rat Thioredoxin-like 5/TRP14 by Western Blot.
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Mouse/Rat Thioredoxin-like 5/TRP14 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects endogenous mouse and rat Thioredoxin-like 5/TRP14 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Thioredoxin-like 5/TRP14
Met1-Asp123
Accession # Q9CQM5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse/Rat Thioredoxin-like 5/TRP14 by Western Blot. View Larger

Detection of Mouse/Rat Thioredoxin-like 5/TRP14 by Western Blot. Western blot shows lysates of A20 mouse B cell lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse/Rat Thioredoxin-like 5/TRP14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3769) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Thioredoxin-like 5/TRP14 at approximately 14 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thioredoxin-like 5/TRP14

Thioredoxin-like 5, also known as a 14 kDa thioredoxin-related protein (TRP14) is a 14 kDa cytosolic redox regulatory molecule that belongs to the thioredoxin family. It contains a thioredoxin-like redox-sensitive active site motif (Cys-Pro-Asp-Cys). In its reduced form, the active site cysteine residues reduce protein disulfide bonds. The resulting active site disulfide is subsequently reduced in a reaction catalyzed by the NADPH-dependent thioredoxin reductase 1. Thioredoxin-like 5 is ubiquitously expressed and acts on protein targets different from that of Thioredoxin-1. The amino acid sequence of mouse Thioredoxin-like 5 is 96% identical to that of rat Thioredoxin-like 5.

Entrez Gene IDs
84817 (Human); 52700 (Mouse)
Alternate Names
MGC14353; Protein 42-9-9; thioredoxin (Trx)-related protein, 14 kDa; thioredoxin domain containing 17; thioredoxin domain-containing protein 17; Thioredoxin like 5; Thioredoxin-like 5; Thioredoxin-like protein 5; TRP14; TRP1414 kDa thioredoxin-related protein; TXNDC17; TXNL5

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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