Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Blockade of Receptor-ligand Interaction, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunofluorescence, Immunocytochemistry, ELISA Development (Capture), IHC-F

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TrkC Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TrkC
Cys32-Thr429
Accession # Q6VNS1

Specificity

Detects mouse TrkC in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) TrkC is observed and less than 2% cross‑reactivity with recombinant mouse TrkB, recombinant rat TrkA, and rhTrkA is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse/Rat TrkC Antibody

Detection of Mouse and Rat TrkC antibody by Western Blot.

Detection of Mouse and Rat TrkC by Western Blot.

Western blot shows lysates of mouse brain (cerebellum) tissue, mouse brain (cortex) tissue, and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat TrkC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1404) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TrkC at approximately 100 and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse TrkC antibody by Simple WesternTM.

Detection of Mouse TrkC by Simple WesternTM.

Simple Western lane view shows lysates of mouse brain tissue, loaded at 0.2 mg/mL. Specific bands were detected for TrkC at approximately 111 and 159 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse/Rat TrkC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1404) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse TrkC by Immunocytochemistry/ Immunofluorescence

Detection of Mouse TrkC by Immunocytochemistry/ Immunofluorescence

Cra1/+ mice show no evidence of proprioceptive sensory neuron loss at symptomatic ages.A) Proprioceptive sensory neuron labeling and quantification with parvalbumin, (B) ER81, and (C) TrkC shows no difference between of +/+ and Cra1/+ mice at 6 months of age (Scale bar  = 20 µm). D) Parvalbumin labeling of proprioceptive sensory neuron fibers within the spinal cord shows the central projection of these sensory neurons is intact in Cra1/+ mice (Scale bar  = 20 µm; N = 3 animals per genotype). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0016753), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse TrkC by Immunocytochemistry/ Immunofluorescence

Detection of Mouse TrkC by Immunocytochemistry/ Immunofluorescence

Cra1/+ mice show no evidence of proprioceptive sensory neuron loss at symptomatic ages.A) Proprioceptive sensory neuron labeling and quantification with parvalbumin, (B) ER81, and (C) TrkC shows no difference between of +/+ and Cra1/+ mice at 6 months of age (Scale bar  = 20 µm). D) Parvalbumin labeling of proprioceptive sensory neuron fibers within the spinal cord shows the central projection of these sensory neurons is intact in Cra1/+ mice (Scale bar  = 20 µm; N = 3 animals per genotype). Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0016753), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse TrkC by Immunocytochemistry/ Immunofluorescence

Detection of Mouse TrkC by Immunocytochemistry/ Immunofluorescence

Ntrk3 in the Glomerulus. Fresh frozen tissues were sectioned and fixed in methanol followed by immunostaining with goat anti-Ntrk3, rabbit anti-WT1, or rabbit anti-Nephrin, as indicated. PTIP+ sections (A–C, G–I) showed strong Ntrk3 staining in all glomeruli, in a pattern similar to Nephrin. The PTIP− kidney sections (D–F, J–L) showed much lower levels of Ntrk3 protein in glomeruli. All micrographs were taken at manually set, equal exposures. Right panels (C, F, I, L) are overlays of Ntrk3 and WT1 or Ntrk3 and Nephrin and are counterstained with DAPI (blue) to visualize all cell nuclei. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/21060806), licensed under a CC0-1.0 license. Not internally tested by R&D Systems.

Applications for Mouse/Rat TrkC Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 2-5 µg/mL of this antibody will block 50% of the binding of 1 ng/mL of Recombinant Human NT-3 (Catalog # 267-N3) to immobilized Recombinant Mouse TrkC (Catalog # 1404-TC) coated at 2 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (cortex)

Simple Western

25 µg/mL
Sample: Mouse brain tissue

Western Blot

0.5 µg/mL
Sample: Mouse brain (cerebellum) tissue, mouse brain (cortex) tissue, and rat brain tissue

Reviewed Applications

Read 3 reviews rated 4.7 using AF1404 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TrkC

The neurotrophins, including NGF, BDNF, NT‑3 and NT‑4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). p75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low affinity to transduce cellular signaling pathways that synergize or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT‑3, TrkB binds BDNF, NT‑3 and NT‑4/5, and TrkC only binds NT‑3 (1‑2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, Trks are highly conserved between species with the extracellular domains of human and mouse TrkC showing 94% amino acid sequence identity (5). The proteins also exhibit cross-species activity. The primary location of TrkC expression is in the nervous system and, specifically, in regions of the CNS. Low level TrkC expression has also been observed in a wide variety of tissues outside the nervous system (6).

References

  1. Huang, E.J. and L.F. Reichardt (2003) Annu. Rev. Biochem. 72:609.
  2. Dechant, G. (2001) Cell Tissue Res. 305:229.
  3. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.
  4. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  5. Menn, B. et al. (1998) J. Comp. Neurol. 401:47.
  6. Shelton, D. et al. (1995) J. Neurosci. 15:477.

Long Name

Neurotrophic Tyrosine Kinase Receptor C

Alternate Names

NTRK3

Entrez Gene IDs

4916 (Human); 18213 (Mouse); 29613 (Rat)

Gene Symbol

NTRK3

UniProt

Q6VNS1

Additional TrkC Products

Product Documents for Mouse/Rat TrkC Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat TrkC Antibody

For research use only

Related Research Areas

Receptor Tyrosine Kinases (RTKs)

Citations for Mouse/Rat TrkC Antibody

Customer Reviews for Mouse/Rat TrkC Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
33%
3 Stars
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Mouse/Rat TrkC Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adult brain
    Species: Mouse
    Verified Customer | Posted 07/13/2021
    Mouse/Rat TrkC Antibody AF1404
  • Mouse/Rat TrkC Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: brain lysate
    Species: Mouse
    Verified Customer | Posted 06/29/2021
    Mouse/Rat TrkC Antibody AF1404
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23516305
    Species: Mouse
    Verified Customer | Posted 01/05/2015

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