Mouse/Rat TrkC Antibody

(17 citations)   
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse TrkC in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) TrkC is observed and less than 2% cross‑reactivity with recombinant mouse TrkB, recombinant rat TrkA, and rhTrkA is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse TrkC
    Cys32-Thr429
    Accession # Q6VNS1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    25 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of mouse brain (cortex)
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 2-5 µg/mL of this antibody will block 50% of the binding of 1 ng/mL of Recombinant Human NT-3 (Catalog # 267-N3) to immobilized Recombinant Mouse TrkC (Catalog # 1404-TC) coated at 2 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse and Rat TrkC by Western Blot. Western blot shows lysates of mouse brain (cerebellum) tissue, mouse brain (cortex) tissue, and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat TrkC Antigen Affinity-purified Polyclonal Antibody
(Catalog # AF1404) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TrkC at approximately 100 and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse TrkC by Simple WesternTM. Simple Western lane view shows lysates of mouse brain tissue, loaded at 0.2 mg/mL. Specific bands were detected for TrkC at approximately 111 and 159 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse/Rat TrkC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1404) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TrkC

The neurotrophins, including NGF, BDNF, NT‑3 and NT‑4/5, constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptor tyrosine kinases (1, 2). p75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low affinity to transduce cellular signaling pathways that synergize or antagonize those activated by the Trk receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF and NT‑3, TrkB binds BDNF, NT‑3 and NT‑4/5, and TrkC only binds NT‑3 (1‑2). All Trk family proteins share a conserved, complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). At the protein sequence level, Trks are highly conserved between species with the extracellular domains of human and mouse TrkC showing 94% amino acid sequence identity (5). The proteins also exhibit cross-species activity. The primary location of TrkC expression is in the nervous system and, specifically, in regions of the CNS. Low level TrkC expression has also been observed in a wide variety of tissues outside the nervous system (6).

  • References:
    1. Huang, E.J. and L.F. Reichardt (2003) Annu. Rev. Biochem. 72:609.
    2. Dechant, G. (2001) Cell Tissue Res. 305:229.
    3. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.
    4. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
    5. Menn, B. et al. (1998) J. Comp. Neurol. 401:47.
    6. Shelton, D. et al. (1995) J. Neurosci. 15:477.
  • Long Name:
    Neurotrophic Tyrosine Kinase Receptor C
  • Entrez Gene IDs:
    4916 (Human); 18213 (Mouse); 29613 (Rat)
  • Alternate Names:
    EC 2.7.10; EC 2.7.10.1; ETS related protein-neurotrophic receptor tyrosine kinase fusion protein; ETV6-NTRK3 fusion; gp145(trkC); GP145-TrkC; Neurotrophic tyrosine kinase receptor type 3; neurotrophic tyrosine kinase, receptor, type 3; NT 3 receptor; NT-3 growth factor receptor; NTRK3; TrkC tyrosine kinase; TrkC; trk-C; TRKCneurotrophin 3 receptor; tyrosine kinase receptor C
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Genetic ablation of GINIP-expressing primary sensory neurons strongly impairs Formalin-evoked pain
    Authors: L Urien, S Gaillard, L Lo Re, P Malapert, M Bohic, A Reynders, A Moqrich
    Sci Rep, 2017;7(0):43493.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  2. Merkel Cell-Driven BDNF Signaling Specifies SAI Neuron Molecular and Electrophysiological Phenotypes
    Authors: EG Reed-Geagh, MC Wright, LA See, PC Adelman, KH Lee, HR Koerber, SM Maricich
    J Neurosci, 2016;36(15):4362-76.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  3. Tropomyosin Receptor Kinase C Targeted Delivery of a Peptidomimetic Ligand-Photosensitizer Conjugate Induces Antitumor Immune Responses Following Photodynamic Therapy
    Sci Rep, 2016;6(0):37209.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  4. Distinct adhesion-independent functions of beta-catenin control stage-specific sensory neurogenesis and proliferation.
    Authors: Gay M, Valenta T, Herr P, Paratore-Hari L, Basler K, Sommer L
    BMC Biol, 2015;13(0):24.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
    Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
    Nat Neurosci, 2015;18(1):145-53.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  6. Egr3-dependent muscle spindle stretch receptor intrafusal muscle fiber differentiation and fusimotor innervation homeostasis.
    Authors: Oliveira Fernandes M, Tourtellotte W
    J Neurosci, 2015;35(14):5566-78.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh/Frozen
  7. Uncoupling of molecular maturation from peripheral target innervation in nociceptors expressing a chimeric TrkA/TrkC receptor.
    Authors: Gorokhova S, Gaillard S, Urien L, Malapert P, Legha W, Baronian G, Desvignes J, Alonso S, Moqrich A
    PLoS Genet, 2014;10(2):e1004081.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  8. ADAM metalloproteases promote a developmental switch in responsiveness to the axonal repellant Sema3A.
    Authors: Romi E, Gokhman I, Wong E, Antonovsky N, Ludwig A, Sagi I, Saftig P, Tessier-Lavigne M, Yaron A
    Nat Commun, 2014;5(0):4058.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  9. Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling.
    Authors: Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R
    J Neurosci, 2013;33(12):5399-410.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  10. Positional differences of axon growth rates between sensory neurons encoded by Runx3.
    EMBO J., 2012;31(18):3718-29.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  11. Npn-1 contributes to axon-axon interactions that differentially control sensory and motor innervation of the limb.
    Authors: Huettl RE, Soellner H, Bianchi E, Novitch BG, Huber AB
    PLoS Biol., 2011;9(2):e1001020.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  12. Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling.
    Authors: Vaegter CB, Jansen P, Fjorback AW
    Nat. Neurosci., 2011;14(1):54-61.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  13. Reduced spiral ganglion neuronal loss by adjunctive neurotrophin-3 in experimental pneumococcal meningitis.
    Authors: Demel C, Hoegen T, Giese A
    J Neuroinflammation, 2011;8(1):7.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  14. Neurotrophin-3 production promotes human neuroblastoma cell survival by inhibiting TrkC-induced apoptosis.
    Authors: Bouzas-Rodriguez J, Cabrera JR, Delloye-Bourgeois C, Ichim G, Delcros JG, Raquin MA, Rousseau R, Combaret V, Benard J, Tauszig-Delamasure S, Mehlen P
    J. Clin. Invest., 2010;120(3):850-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  15. Runx3 is required for the specification of TrkC-expressing mechanoreceptive trigeminal ganglion neurons.
    Authors: Senzaki K, Ozaki S, Yoshikawa M, Ito Y, Shiga T
    Mol. Cell. Neurosci., 2010;43(3):296-307.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  16. Immunohistochemical analysis of acid-sensing ion channel 2 expression in rat dorsal root ganglion and effects of axotomy.
    Authors: Kawamata T, Ninomiya T, Toriyabe M, Yamamoto J, Niiyama Y, Omote K, Namiki A
    Neuroscience, 2006;143(1):175-87.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  17. TRPV2, a capsaicin receptor homologue, is expressed predominantly in the neurotrophin-3-dependent subpopulation of primary sensory neurons.
    Authors: Tamura S, Morikawa Y, Senba E
    Neuroscience, 2005;130(1):223-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 17 Citations
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