Detects mouse Reg3B in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant rat (rr) Reg3B is observed. In Western blots approximately 30% cross-reactivity with rrReg3B is observed. In direct ELISAs and Western blots, less than 1% cross‑reactivity with rrReg3A, recombinant mouse (rm) Reg3A, and rmReg3G is observed.
Polyclonal Sheep IgG
E. coli-derived recombinant mouse Reg3B Glu27-Gly175 Accession # P35230
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Recombinant Mouse Reg3B
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reg3B in Mouse Small Intestine. Reg3B was detected in immersion fixed frozen sections of mouse small intestine using 10 µg/mL Sheep Anti-Mouse Reg3B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5110) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Reg3B (regenerating islet-derived protein III beta; also PAP-I and HIP) is a secreted, 17 kDa member of the Reg gene family, C-type lectin gene superfamily. It is found in pancreatic acinar cells, intestinal enterocytes and developing spinal motoneurons. Reg3B may play a role in intestinal epithelial cell renewal. The mouse Reg3B precursor is 175 amino acids (aa) in length. It contains a 26 aa signal sequence plus a 149 aa mature region that possesses one C-type lectin domain (aa 38‑173). Mature mouse Reg3B is 59%, 71% and 51% aa identical to the products of three other Reg3 named genes termed RegA, G, and D, respectively. Mature mouse Reg3B is also 91% and 67% aa identical to rat and human Reg3B, respectively.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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