Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot, IHC-F

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all S100A10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse S100A10
Pro2-Lys97
Accession # P08207

Specificity

Detects mouse S100A10 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human (rh) S100A10 is observed and less than 1% cross-reactivity with rhS100B and recombinant mouse S100A9 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse S100A10 Antibody

Detection of Mouse S100A10 antibody by Western Blot.

Detection of Mouse S100A10 by Western Blot.

Western blot shows lysates of mouse lung and MEF mouse embryonic feeder cells. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100A10 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

S100A10 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr).

S100A10 in Mouse Kidney.

S100A10 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse S100A10 antibody by Simple WesternTM.

Detection of Mouse S100A10 by Simple WesternTM.

Simple Western lane view shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells, loaded at 0.2 mg/mL. A specific band was detected for S100A10 at approximately 11 kDa (as indicated) using 1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of S100A10 by Western Blot

Detection of S100A10 by Western Blot

RNS upregulates p11 and inhibits TASK channels in a traumatic model of MN degeneration. g Immunoblots for RhoA and p11 of HNs from P7 rats treated as shown. gapdh and alpha -tub were the internal controls for qRT-PCR and western blotting, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31439839), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of S100A10 by Western Blot

Detection of S100A10 by Western Blot

Neuroprotective effects of siRNAp11 in the SOD1-G93A mouse model of ALS. c. d mRNA (left) and protein (right) levels of p11 and TASK1 in the spinal cord of SOD1-G93A mice and their Non-Tg littermates, at the indicated stages (see schematic on top). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31439839), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of S100A10 by Western Blot

Detection of S100A10 by Western Blot

Cell-type specific p11 dysregulation is pivotal in a traumatic model of MN degeneration. e Western blots (top) and plot (bottom, left) showing p11 upregulation in the injured (Inj) relative to the intact (Int) HN. Empty circle, sham condition. Bottom, right, qRT-PCR analysis. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31439839), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of S100A10 by Western Blot

Detection of S100A10 by Western Blot

Cell-type specific p11 dysregulation is pivotal in a traumatic model of MN degeneration. h Effect of microinjection of indicated LVVs in the midline between both HNs c on protein and mRNA levels for p11 in HNs. Neocortex was a control for systemic transduction. gapdh and alpha -tub were internal controls for qRT-PCR and western blotting, respectively.\ Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31439839), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse S100A10 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney

Simple Western

1 µg/mL
Sample: Mouse lung tissue and MEF mouse embryonic feeder cells

Western Blot

0.1 µg/mL
Sample: Mouse lung and MEF mouse embryonic feeder cells

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100A10

S100A10 is a member of the S100 family of EF hand calcium-binding proteins. It is primarily found in mast cells as well as heterotetrameric complexes (two annexin A2 subunits and two S100 A10 subunits) that are present intracellularly at the plasma membrane or on the extracellular cell surface. Extracellular S100A10 is a key plasminogen receptor that plays an important role in cellular plasmin production and cellular invasiveness.

Long Name

S100 Calcium Binding Protein

Alternate Names

annexin II ligand, calpactin I, light polypeptide, ANX2L, ANX2LG, CAL1LGP11,42C, Calpactin I light chain, Calpactin-1 light chain, Cellular ligand of annexin II, CLP11Ca[1], MGC111133, p10, p10 protein, p11, protein S100-A10, S100 calcium binding protein A10, S100 calcium binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11)), S100 calcium-binding protein A10, S100 calcium-binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11))

Entrez Gene IDs

6281 (Human); 20194 (Mouse)

Gene Symbol

S100A10

UniProt

P08207

Additional S100A10 Products

Product Documents for Mouse S100A10 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse S100A10 Antibody

For research use only

Citations for Mouse S100A10 Antibody

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Protocols

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