Mouse Sca-1/Ly6 Antibody

Name: Mouse Sca-1/Ly6 Antibody
Brand: R&D Systems
SKU: AF1226-SP
Rating: 5 (1 reviews)

AF1226 16 Citations 1 Review Datasheet / COA / SDS

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AF1226
AF1226-SP

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Mouse Sca-1/Ly6 Antibody in Immunocytochemistry

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Citations (16)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Sca-1/Ly6 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Sca-1/Ly6 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Sca-1 C-terminally truncated Ly-6E allele
Leu27-Gly119
Accession # CAA28351

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Sca‑1/Ly6

Flow Cytometry

2.5 µg/10⁶ cells

Mouse splenocytes

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry

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Sca‑1/Ly6 in Mouse Splenocytes. Sca-1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse Sca-1/Ly6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sca-1/Ly6

This antibody reacts with Sca-1, an 18 kDa phosphatidylinositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family (1). Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly‑6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non‑hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).

References

Van de Rijn, M. et al. (1989) Proc. Natl.Acad. Sci. USA 86:4634.
Spangrude, G.I. et al. (1988) Science 241:58.
Spangrude, G.I. et al. (1993) Blood 82:3327.
Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
Yamamoto, Y. et al. (1996) Blood 88:445.
Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
Welm, B.E. et al. (2002) Dev. Biol. 245:42.
Codias, E.K. et al. (1990) J. Immunol. 144:2197.
Malek, T.R. et al. (1986) J. Exp. Med. 164:709 .
Codias, E.K. et al. (1990) J. Immunol. 145:1407.
Flood, P.M. et al. (1990) J. Exp. Med. 172:115.

Long Name

Stem Cell Antigen-1/Lymphocyte Antigen 6

Entrez Gene IDs

110454 (Mouse)

Alternate Names

C6orf23; G6D; Ly6; Ly-6; LY6-D; LY6G6D; lymphocyte antigen 6 family member G6D; MEGT1; NG25; Sca1; Sca-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Sca-1/Ly6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2018;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms
Authors: L Golle, HU Gerth, K Beul, B Heitplatz, P Barth, M Fobker, H Pavenstädt, GS Di Marco, M Brand
Sci Rep, 2017;7(1):9444.
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry
Systemic application of sirolimus prevents neointima formation not via a direct anti-proliferative effect but via its anti-inflammatory properties
Authors: JM Daniel, J Dutzmann, H Brunsch, J Bauersachs, R Braun-Dull, DG Sedding
Int. J. Cardiol., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Dipeptidyl Peptidase-4 Regulates Hematopoietic Stem Cell Activation in Response to Chronic Stress
Authors: E Zhu, L Hu, H Wu, L Piao, G Zhao, A Inoue, W Kim, C Yu, W Xu, YK Bando, X Li, Y Lei, CN Hao, K Takeshita, WS Kim, K Okumura, T Murohara, M Kuzuya, XW Cheng
J Am Heart Assoc, 2017;6(7):.
Pluripotent stem cells derived from mouse and human white mature adipocytes.
Authors: Jumabay M, Abdmaulen R, Ly A, Cubberly M, Shahmirian L, Heydarkhan-Hagvall S, Dumesic D, Yao Y, Bostrom K
Stem Cells Transl Med, 2014;3(2):161-71.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Distinct fibroblast lineages determine dermal architecture in skin development and repair.
Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
Nature, 2013;504(7479):277-81.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Age dependence of lung mesenchymal stromal cell dynamics following pneumonectomy.
Authors: Paxson J, Gruntman A, Davis A, Parkin C, Ingenito E, Hoffman A
Stem Cells Dev, 2013;22(24):3214-25.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Stromal cell-derived factor-1 enhances distraction osteogenesis-mediated skeletal tissue regeneration through the recruitment of endothelial precursors.
Authors: Fujio M, Yamamoto A, Ando Y, Shohara R, Kinoshita K, Kaneko T, Hibi H, Ueda M
Bone, 2011;49(4):693-700.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Structural basis of digoxin that antagonizes RORgamma t receptor activity and suppresses Th17 cell differentiation and interleukin (IL)-17 production.
Authors: Fujita-Sato S, Ito S, Isobe T, Ohyama T, Wakabayashi K, Morishita K, Ando O, Isono F
J. Biol. Chem., 2011;286(36):31409-17.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Three-dimensional culture of mouse renal carcinoma cells in agarose macrobeads selects for a subpopulation of cells with cancer stem cell or cancer progenitor properties.
Authors: Smith BH, Gazda LS, Conn BL
Cancer Res., 2011;71(3):716-24.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Prominin-1/CD133+ lung epithelial progenitors protect from bleomycin-induced pulmonary fibrosis.
Authors: Germano D, Blyszczuk P, Valaperti A, Kania G, Dirnhofer S, Landmesser U, Luscher TF, Hunziker L, Zulewski H, Eriksson U
Am. J. Respir. Crit. Care Med., 2009;179(10):939-49.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Intestinal epithelial cell up-regulation of LY6 molecules during colitis results in enhanced chemokine secretion.
Authors: Flanagan K, Modrusan Z, Cornelius J, Chavali A, Kasman I, Komuves L, Mo L, Diehl L
J. Immunol., 2008;180(6):3874-81.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

A Gata6-Wnt pathway required for epithelial stem cell development and airway regeneration.
Authors: Zhang Y, Goss AM, Cohen ED, Kadzik R, Lepore JJ, Muthukumaraswamy K, Yang J, DeMayo FJ, Whitsett JA, Parmacek MS, Morrisey EE
Nat. Genet., 2008;40(7):862-70.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

The recruitment of primitive Lin(-) Sca-1(+), CD34(+), c-kit(+) and CD271(+) cells during the early intraperitoneal foreign body reaction.
Authors: Vranken I, De Visscher G, Lebacq A, Verbeken E, Flameng W
Biomaterials, 2007;29(7):797-808.
Species: Rat
Sample Types: Whole Cells
Applications: ICC

Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS coronavirus (SARS-CoV) infection in vitro.
Authors: Ling TY, Kuo MD, Li CL, Yu AL, Huang YH, Wu TJ, Lin YC, Chen SH, Yu J
Proc. Natl. Acad. Sci. U.S.A., 2006;103(25):9530-5.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Identification of bronchioalveolar stem cells in normal lung and lung cancer.
Authors: Kim CF, Jackson EL, Woolfenden AE, Lawrence S, Babar I, Vogel S, Crowley D, Bronson RT, Jacks T
Cell, 2005;121(6):823-35.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC