hh Mouse Sca-1/Ly6 Antibody AF1226: R&D Systems

Mouse Sca-1/Ly6 Antibody

(13 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Sca-1/Ly6 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Sca-1 C-terminally truncated Ly-6E allele
    Leu27-Gly119
    Accession # CAA28351
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Sca‑1/Ly6
  • Flow Cytometry
    2.5 µg/106 cells
    Mouse splenocytes
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Sca‑1/Ly6 in Mouse Splenocytes. Sca‑1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse Sca‑1/Ly6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sca-1/Ly6

This antibody reacts with Sca-1, an 18 kDa phosphatidylinositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family (1). Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly‑6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non‑hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).

  • References:
    1. Van de Rijn, M. et al. (1989) Proc. Natl.Acad. Sci. USA 86:4634.
    2. Spangrude, G.I. et al. (1988) Science 241:58.
    3. Spangrude, G.I. et al. (1993) Blood 82:3327.
    4. Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
    5. Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
    6. Yamamoto, Y. et al. (1996) Blood 88:445.
    7. Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
    8. Welm, B.E. et al. (2002) Dev. Biol. 245:42.
    9. Codias, E.K. et al. (1990) J. Immunol. 144:2197.
    10. Malek, T.R. et al. (1986) J. Exp. Med. 164:709 .
    11. Codias, E.K. et al. (1990) J. Immunol. 145:1407.
    12. Flood, P.M. et al. (1990) J. Exp. Med. 172:115.
  • Long Name:
    Stem Cell Antigen-1/Lymphocyte Antigen 6
  • Entrez Gene IDs:
    110454 (Mouse)
  • Alternate Names:
    Ly6; Ly-6; Sca1; Sca-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Dipeptidyl Peptidase-4 Regulates Hematopoietic Stem Cell Activation in Response to Chronic Stress
    Authors: E Zhu, L Hu, H Wu, L Piao, G Zhao, A Inoue, W Kim, C Yu, W Xu, YK Bando, X Li, Y Lei, CN Hao, K Takeshita, WS Kim, K Okumura, T Murohara, M Kuzuya, XW Cheng
    J Am Heart Assoc, 2017;6(7):.
  2. Pluripotent stem cells derived from mouse and human white mature adipocytes.
    Authors: Jumabay M, Abdmaulen R, Ly A, Cubberly M, Shahmirian L, Heydarkhan-Hagvall S, Dumesic D, Yao Y, Bostrom K
    Stem Cells Transl Med, 2014;3(2):161-71.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  3. Age dependence of lung mesenchymal stromal cell dynamics following pneumonectomy.
    Authors: Paxson J, Gruntman A, Davis A, Parkin C, Ingenito E, Hoffman A
    Stem Cells Dev, 2013;22(24):3214-25.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013;504(7479):277-81.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. Three-dimensional culture of mouse renal carcinoma cells in agarose macrobeads selects for a subpopulation of cells with cancer stem cell or cancer progenitor properties.
    Authors: Smith BH, Gazda LS, Conn BL
    Cancer Res., 2011;71(3):716-24.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  6. Structural basis of digoxin that antagonizes RORgamma t receptor activity and suppresses Th17 cell differentiation and interleukin (IL)-17 production.
    Authors: Fujita-Sato S, Ito S, Isobe T, Ohyama T, Wakabayashi K, Morishita K, Ando O, Isono F
    J. Biol. Chem., 2011;286(36):31409-17.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Stromal cell-derived factor-1 enhances distraction osteogenesis-mediated skeletal tissue regeneration through the recruitment of endothelial precursors.
    Authors: Fujio M, Yamamoto A, Ando Y, Shohara R, Kinoshita K, Kaneko T, Hibi H, Ueda M
    Bone, 2011;49(4):693-700.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  8. Prominin-1/CD133+ lung epithelial progenitors protect from bleomycin-induced pulmonary fibrosis.
    Authors: Germano D, Blyszczuk P, Valaperti A, Kania G, Dirnhofer S, Landmesser U, Luscher TF, Hunziker L, Zulewski H, Eriksson U
    Am. J. Respir. Crit. Care Med., 2009;179(10):939-49.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  9. Intestinal epithelial cell up-regulation of LY6 molecules during colitis results in enhanced chemokine secretion.
    Authors: Flanagan K, Modrusan Z, Cornelius J, Chavali A, Kasman I, Komuves L, Mo L, Diehl L
    J. Immunol., 2008;180(6):3874-81.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  10. A Gata6-Wnt pathway required for epithelial stem cell development and airway regeneration.
    Authors: Zhang Y, Goss AM, Cohen ED, Kadzik R, Lepore JJ, Muthukumaraswamy K, Yang J, DeMayo FJ, Whitsett JA, Parmacek MS, Morrisey EE
    Nat. Genet., 2008;40(7):862-70.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  11. The recruitment of primitive Lin(-) Sca-1(+), CD34(+), c-kit(+) and CD271(+) cells during the early intraperitoneal foreign body reaction.
    Authors: Vranken I, De Visscher G, Lebacq A, Verbeken E, Flameng W
    Biomaterials, 2007;29(7):797-808.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  12. Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS coronavirus (SARS-CoV) infection in vitro.
    Authors: Ling TY, Kuo MD, Li CL, Yu AL, Huang YH, Wu TJ, Lin YC, Chen SH, Yu J
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(25):9530-5.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  13. Identification of bronchioalveolar stem cells in normal lung and lung cancer.
    Authors: Kim CF, Jackson EL, Woolfenden AE, Lawrence S, Babar I, Vogel S, Crowley D, Bronson RT, Jacks T
    Cell, 2005;121(6):823-35.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 13 Citations
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Flow Cytometry Mouse Sca-1/Ly6 Antibody AF1226
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Flow Cytometry Mouse Sca-1/Ly6 Antibody AF1226
Flow Cytometry: Mouse Sca-1/Ly6 Antibody [AF1226]

Summary

ApplicationFlow Cytometry
Sample TestedBone marrow
SpeciesMouse

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