Mouse Sca-1/Ly6 Antibody
Mouse Sca-1/Ly6 Antibody Summary
Leu27-Gly119
Accession # CAA28351
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Sca‑1/Ly6 in Mouse Splenocytes. Sca-1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse Sca-1/Ly6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sca-1/Ly6
This antibody reacts with Sca-1, an 18 kDa phosphatidylinositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family (1). Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly‑6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non‑hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).
- Van de Rijn, M. et al. (1989) Proc. Natl.Acad. Sci. USA 86:4634.
- Spangrude, G.I. et al. (1988) Science 241:58.
- Spangrude, G.I. et al. (1993) Blood 82:3327.
- Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
- Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
- Yamamoto, Y. et al. (1996) Blood 88:445.
- Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
- Welm, B.E. et al. (2002) Dev. Biol. 245:42.
- Codias, E.K. et al. (1990) J. Immunol. 144:2197.
- Malek, T.R. et al. (1986) J. Exp. Med. 164:709 .
- Codias, E.K. et al. (1990) J. Immunol. 145:1407.
- Flood, P.M. et al. (1990) J. Exp. Med. 172:115.
Product Datasheets
Citations for Mouse Sca-1/Ly6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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CXCL12/CXCR4-Rac1-mediated migration of osteogenic precursor cells contributes to pathological new bone formation in ankylosing spondylitis
Authors: H Cui, Z Li, S Chen, X Li, D Chen, J Wang, Z Li, W Hao, F Zhong, K Zhang, Z Zheng, Z Zhan, H Liu
Science Advances, 2022;8(14):eabl8054.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Fecal microbiota transplantation ameliorates atherosclerosis in mice with C1q/TNF-related protein 9 genetic deficiency
Authors: ES Kim, BH Yoon, SM Lee, M Choi, EH Kim, BW Lee, SY Kim, CG Pack, YH Sung, IJ Baek, CH Jung, TB Kim, JY Jeong, CH Ha
Experimental & Molecular Medicine, 2022;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Bone marrow-independent adventitial macrophage progenitor cells contribute to angiogenesis
Authors: F Kleefeldt, B Upcin, H Bömmel, C Schulz, G Eckner, J Allmanritt, J Bauer, B Braunger, U Rueckschlo, S Ergün
Cell Death & Disease, 2022;13(3):220.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Distinct Expression Patterns of Cxcl12 in Mesenchymal Stem Cell Niches of Intact and Injured Rodent Teeth
Authors: P Pagella, C Nombela-Ar, TA Mitsiadis
International Journal of Molecular Sciences, 2021;22(6):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2018;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Systemic application of sirolimus prevents neointima formation not via a direct anti-proliferative effect but via its anti-inflammatory properties
Authors: JM Daniel, J Dutzmann, H Brunsch, J Bauersachs, R Braun-Dull, DG Sedding
Int. J. Cardiol., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Dipeptidyl Peptidase-4 Regulates Hematopoietic Stem Cell Activation in Response to Chronic Stress
Authors: E Zhu, L Hu, H Wu, L Piao, G Zhao, A Inoue, W Kim, C Yu, W Xu, YK Bando, X Li, Y Lei, CN Hao, K Takeshita, WS Kim, K Okumura, T Murohara, M Kuzuya, XW Cheng
J Am Heart Assoc, 2017;6(7):.
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Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms
Authors: L Golle, HU Gerth, K Beul, B Heitplatz, P Barth, M Fobker, H Pavenstädt, GS Di Marco, M Brand
Sci Rep, 2017;7(1):9444.
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry -
Pluripotent stem cells derived from mouse and human white mature adipocytes.
Authors: Jumabay M, Abdmaulen R, Ly A, Cubberly M, Shahmirian L, Heydarkhan-Hagvall S, Dumesic D, Yao Y, Bostrom K
Stem Cells Transl Med, 2014;3(2):161-71.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Age dependence of lung mesenchymal stromal cell dynamics following pneumonectomy.
Authors: Paxson J, Gruntman A, Davis A, Parkin C, Ingenito E, Hoffman A
Stem Cells Dev, 2013;22(24):3214-25.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Distinct fibroblast lineages determine dermal architecture in skin development and repair.
Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
Nature, 2013;504(7479):277-81.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Three-dimensional culture of mouse renal carcinoma cells in agarose macrobeads selects for a subpopulation of cells with cancer stem cell or cancer progenitor properties.
Authors: Smith BH, Gazda LS, Conn BL
Cancer Res., 2011;71(3):716-24.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Structural basis of digoxin that antagonizes RORgamma t receptor activity and suppresses Th17 cell differentiation and interleukin (IL)-17 production.
Authors: Fujita-Sato S, Ito S, Isobe T, Ohyama T, Wakabayashi K, Morishita K, Ando O, Isono F
J. Biol. Chem., 2011;286(36):31409-17.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Stromal cell-derived factor-1 enhances distraction osteogenesis-mediated skeletal tissue regeneration through the recruitment of endothelial precursors.
Authors: Fujio M, Yamamoto A, Ando Y, Shohara R, Kinoshita K, Kaneko T, Hibi H, Ueda M
Bone, 2011;49(4):693-700.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Prominin-1/CD133+ lung epithelial progenitors protect from bleomycin-induced pulmonary fibrosis.
Authors: Germano D, Blyszczuk P, Valaperti A, Kania G, Dirnhofer S, Landmesser U, Luscher TF, Hunziker L, Zulewski H, Eriksson U
Am. J. Respir. Crit. Care Med., 2009;179(10):939-49.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Intestinal epithelial cell up-regulation of LY6 molecules during colitis results in enhanced chemokine secretion.
Authors: Flanagan K, Modrusan Z, Cornelius J, Chavali A, Kasman I, Komuves L, Mo L, Diehl L
J. Immunol., 2008;180(6):3874-81.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
A Gata6-Wnt pathway required for epithelial stem cell development and airway regeneration.
Authors: Zhang Y, Goss AM, Cohen ED, Kadzik R, Lepore JJ, Muthukumaraswamy K, Yang J, DeMayo FJ, Whitsett JA, Parmacek MS, Morrisey EE
Nat. Genet., 2008;40(7):862-70.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
The recruitment of primitive Lin(-) Sca-1(+), CD34(+), c-kit(+) and CD271(+) cells during the early intraperitoneal foreign body reaction.
Authors: Vranken I, De Visscher G, Lebacq A, Verbeken E, Flameng W
Biomaterials, 2007;29(7):797-808.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS coronavirus (SARS-CoV) infection in vitro.
Authors: Ling TY, Kuo MD, Li CL, Yu AL, Huang YH, Wu TJ, Lin YC, Chen SH, Yu J
Proc. Natl. Acad. Sci. U.S.A., 2006;103(25):9530-5.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Identification of bronchioalveolar stem cells in normal lung and lung cancer.
Authors: Kim CF, Jackson EL, Woolfenden AE, Lawrence S, Babar I, Vogel S, Crowley D, Bronson RT, Jacks T
Cell, 2005;121(6):823-35.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
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