Mouse Semaphorin 3B Antibody (904201)

R&D Systems | Catalog # MAB5440

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 904201
View all Semaphorin 3B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 3B 
Pro26-Pro722
Accession # Q62177

Specificity

Detects mouse Semaphorin 3B in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Semaphorin 3B Antibody (904201)

Detection of Mouse Semaphorin 3B antibody by Western Blot.

Detection of Mouse Semaphorin 3B by Western Blot.

Western blot shows lysates of NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Semaphorin 3B Monoclonal Antibody (Catalog # MAB5440) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Semaphorin 3B at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Semaphorin 3B antibody in NS0 Mouse Cell Line by Immunocytochemistry (ICC).

Semaphorin 3B in NS0 Mouse Cell Line.

Semaphorin 3B was detected in immersion fixed NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B (upper panel) or irrelevant transfectant (lower panel) using Rat Anti-Mouse Semaphorin 3B Monoclonal Antibody (Catalog # MAB5440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Semaphorin 3B by Western Blot

Detection of Semaphorin 3B by Western Blot

Expression of Sema-3B and Nrp-1 in B16F10 mouse melanoma model. a Expression of Sema-3B in B16F10 tumour cells. Total protein extracts from B16F10 cells cultured in vitro or isolated ex vivo from tumour grafts were analysed by western blot using anti-Sema-3B mAb. b Surface expression of Nrp-1 on CD4+ and CD8+ T cells infiltrating B16F10 melanoma engrafted in C57BL/6 mice. TIL from individual tumours were isolated at day 15 after tumour cell inoculation. T lymphocytes from spleens and TdLN of tumour-bearing mice were analysed in parallel. Percentages of positive cells are included. Right: percentages of Nrp-1+ cells among CD8+ and CD4+ T cells in TIL (n = 29 and 31), splenocytes (n = 29 and 31) and TdLN (n = 11 and 16). c Expression of Nrp-1 and FoxP3 in CD4+ T cells. T lymphocytes from tumours (n = 20), spleens (n = 22) and TdLN (n = 11) of B16F10 melanoma-bearing mice were analysed at day 15 by flow cytometry. Right: percentages of Nrp-1 among FoxP3+ and FoxP3− CD4+ T lymphocytes from B16F10. d Expression of CD44 and CD62L on Nrp-1+ and Nrp-1− CD8+ T cells from B16F10 TIL. Right: Distribution of naive, effector and memory T cells populations among Nrp-1− and Nrp-1+ CD8+ TIL (n = 10). Results are representative of 3–5 independent experiments. Means ± SEM one-way ANOVA test with Bonferroni correction b or two-way ANOVA test with Bonferroni correction c. *P < 0.05; **P < 0.01; ***P < 0.001. Source data are provided as a Source Data file a, d Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31350404), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Semaphorin 3B Antibody (904201)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NSO mouse myeloma cell line transfected with mouse Semaphorin 3B or irrelevant transfectant

Western Blot

0.5 µg/mL
Sample: NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B

Reviewed Applications

Read 1 review rated 5 using MAB5440 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 3B

Sema3b (Semaphorin 3B) is a 748 aa secreted glycoprotein.  Mouse Sema3b shares 88% and 96% aa identity with human and rat Sema3b, respectively.  The semaphorin family has been demonstrated to play a role in axon guidance.  Additionally, Sema3b has been implicated in bone mass regulation and tumor suppression.

Alternate Names

LUCA-1, SEMA3B, SEMA5, SEMAA, SEMAV

Entrez Gene IDs

7869 (Human); 20347 (Mouse); 363142 (Rat)

Gene Symbol

SEMA3B

UniProt

Q62177 (Mouse)

Additional Semaphorin 3B Products

Product Documents for Mouse Semaphorin 3B Antibody (904201)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Semaphorin 3B Antibody (904201)

For research use only

Related Research Areas

Proteoglycans

Citations for Mouse Semaphorin 3B Antibody (904201)

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  • Mouse Semaphorin 3B Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: NS0 Mouse Cells and NS0 Mouse Cell Line
    Species: Mouse
    Verified Customer | Posted 01/24/2022
    Mouse Semaphorin 3B Antibody (904201) MAB5440

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