Mouse Semaphorin 3C Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1728
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Mouse Semaphorin 3C Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Semaphorin 3C in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) Semaphorin 3B is observed, 5% cross-reactivity with rmSemaphorin 3A, rmSemaphorin 3E, and recombinant human (rh) Semaphorin 3F is observed and less than 1% cross-reactivity with rmSema 6A and rhSema 7A is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 3C
Gln24-Ser751 (Arg48Ala, Arg52Ala)
Accession # Q62181
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Semaphorin 3C Fc Chimera, Truncated (Catalog # 1728-S3)
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (E13.5-15.5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 3C

Semaphorin 3C (Sema 3C; previously semaE) is one of six Class 3 secreted semaphorins which share 40-50% amino acid (aa) identity. Class 3 semaphorins are potent chemorepellents that function in axon and/or vascular guidance during development, and may be upregulated in tumor progression (1, 2). The 751 amino acid (aa) mouse Sema3C is highly modular. It contains a 20 aa signal sequence, an ~500 aa N-terminal Sema domain that forms a beta -propeller structure similar to that found in integrin molecules, a cysteine knot, a furin-type cleavage site, an Ig-like domain, and a C-terminal basic domain (1-3). Covalent dimerization plus cleavage at the C-terminus are required for activity of class 3 semaphorins (4). Mouse Sema 3C shares at least 95% aa identity with human, rat, cow and dog Sema 3C, and 89% and 75% aa sequence identity with chick and zebrafish Sema 3C, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors (1, 2). Sema 3C signaling is transduced by Plexin-D1 indirectly via neuropilin-1 or neuropilin-2 receptors (5). Sema 3C is expressed in all somitic motor neurons, in lung buds and in cardiac neural crest cells during development (1, 5-8). Sema 3C activates integrins in certain cells so, in addition to its repulsive activities, it sometimes acts as a chemoattractant (6, 9). In the developing nervous system, this chemoattraction appears to complement Sema 3A repulsion in adjacent cell layers (1, 6, 7). Sema 3C also provides an attractive force opposing Sema 6A and Sema 6B to guide migration of neural crest endothelial cells to the cardiac outflow tract (10). Consequently, defects in aortic arch formation occur when Sema 3C or Plexin-D1 genes or Sema 3C‑neuropilin interactions are disrupted (5, 11, 12).

References
  1. Hinck, L. (2004) Dev. Cell 7:783.
  2. Neufeld, G. et al. (2005) Front. Biosci. 10:751.
  3. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
  4. Adams, R.H. et al. (1997) EMBO J. 16:6077.
  5. Gitler, A.D. et al. (2004) Dev. Cell 7:107.
  6. Bagnard, D. et al. (1998) Development 125:5043.
  7. Cohen, S. et al. (2005) Eur. J. Neurosci. 21:1767.
  8. Puschel, A. W. et al. (1995) Neuron 14:941.
  9. Herman, J.G. and G.G. Meadows (2007) Int. J. Oncol. 30:1231.
  10. Toyofuku, T. et al. (2008) Dev. Biol. 321:251.
  11. Feiner, L. et al. (2001) Development 128:3061.
  12. Gu, C. et al. (2003) Dev. Cell 5:45.
Entrez Gene IDs
10512 (Human); 20348 (Mouse)
Alternate Names
(semaphorin) 3C; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema E; SEMA3C; SEMAE; Semaphorin 3C; Semaphorin E; semaphorin-3C; semaphorin-E; SEME

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