Mouse Semaphorin 4D/CD100 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Semaphorin 4D/CD100 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) Semaphorin 3A, rhSemaphorin 4B, and rhSemaphrin 6C is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4D/CD100
    Phe24-Thr657
    Accession # NP_038688
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Semaphorin 4D/CD100
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed frozen sections of mouse embryonic eye cup (15 d.p.c.)
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Semaphorin 4D/
CD100 in EL‑4 Mouse Cell Line by Flow Cytometry.
EL‑4 mouse lymphoblast cell line were stained with Sheep Anti-Mouse Semaphorin 4D/CD100 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5235, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 4D/CD100
Semaphorin 4D (Sema4D/CD100, previously sem J, G or C-like 2) is a widely expressed 150 kDa type I transmembrane glycoprotein of the Class 4 Semaphorin family with activity in the immune and nervous systems. The 861 amino acid (aa) Sema4D contains a 710 aa extracellular domain (ECD) with a sema domain and an immunoglobulin-like domain. Within the ECD region used as an immunogen, mouse Sema4D shares 87% and 93% aa identity with human and rat Sema4D, respectively. A soluble, active 120 kDa form is produced proteolytically upon activation of B and T cells. Sema4D binds Plexin-B1, but also CD72 on B and dendritic cells. In developing neurons, Sema-4D assists in guidance. Sema4D is proangiogenic when expressed by tumor-associated macrophages.
  • Entrez Gene IDs:
    10507 (Human); 20354 (Mouse); 306790 (Rat)
  • Alternate Names:
    A8; BB18; CD100 antigen; CD100; CD100M-sema-G; COLL-4; FLJ33485; FLJ34282; FLJ39737; FLJ46484; immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D; MGC169138; MGC169141; M-sema-G; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D; SEMA4D; SEMAJ; Semaphorin 4D; semaphorin-4D
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