Mouse Serpin A8/Angiotensinogen Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Serpin A8/Angiotensinogen in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Serpin A8/Angiotensinogen, rhSerpin A4, recombinant mouse (rm) Serpin A1, A3N, A11, B8, C1, E1, E2, I1, or rmPEDF is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 754438
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Serpin A8/Angiotensinogen
    Asp25-Val477
    Accession # P11859
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Serpin A8/Angiotensinogen by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Serpin A8/Angiotensinogen Monoclonal Antibody (Catalog # MAB6966) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Serpin A8/Angiotensinogen at approximately 52-62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Serpin A8/Angiotensinogen in Mouse Intestine. Serpin A8/Angiotensinogen was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Serpin A8/Angiotensinogen Monoclonal Antibody (Catalog # MAB6966) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A8/Angiotensinogen

Serpin A8 (serine proteinase inhibitor-clade A #8; also angiotensinogen/ANGT) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors.  It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I.  This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II.  Ang II induces vascoconstriction and aldosterone release.  Mature human Serpin A8 is 452 aa in length (aa 34-485).  It contains Ang I (aa 34-43) that is cleaved to create Ang II (aa 34-41).  Serpin A8 may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg.  Over aa 34-485, human Serpin A8 shares 61% aa identity with mouse Serpin A8.

  • Entrez Gene IDs:
    183 (Human); 11606 (Mouse); 24179 (Rat)
  • Alternate Names:
    AGT; alpha-1 antiproteinase, antitrypsin; Angiotensin II; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); Angiotensinogen; ANHU; Aogen; FLJ92595; FLJ97926; PAT; pre-angiotensinogen; serine (or cysteine) proteinase inhibitor; Serpin A8; SERPINA8angiotensin I
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