Mouse Serpin D1/Heparin Cofactor II Antibody

Catalog # Availability Size / Price Qty
Product Details
Supplemental Products

Mouse Serpin D1/Heparin Cofactor II Antibody Summary

Species Reactivity
Detects mouse Serpin D1/Heparin Cofactor II in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Serpin D1/Heparin Cofactor II
Accession # P49182
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Serpin D1/Heparin Cofactor II (Catalog # 1265-PI)
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Serpin D1/
Heparin Cofactor II (Catalog # 1265-PI), see our available Western blot detection antibodies
Measured by its ability to neutralize Recombinant Mouse Serpin D1 (0.475 µg/mL, Catalog # 1265-PI) inhibition of Recombinant Human Coagulation Factor II/Thrombin (0.05 µg/mL, Catalog # 1473-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-AMC (100 µM, Catalog # ES011). The Neutralization Dose (ND50) is typically 1.23 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin D1/Heparin Cofactor II

Serpin D1 is a member of the Serpin superfamily of the serine protease inhibitors (1). Similar to Serpins A5 and C1, it inhibits thrombin and this activity is enhanced by heparin. Interestingly, a C-terminal His-tagged recombinant Serpin D1 had enhanced heparin effect, which was maintained in a plasma-based thrombin inhibition assay (2). Congenital Serpin D1 deficiency is a potential risk factor for thrombosis (3). Mouse Serpin D1 is synthesized as a 478 amino acid precursor with a 23 amino acid signal peptide (4). The mature chain (residues 24 to 478) is secreted.

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Bauman, S.J. et al. (1999) J. Biol. Chem. 274:34556.
  3. Tollefsen, D.M. (2002) Arch. Pathol. Lab. Med. 126:1394.
  4. Zhang, G.S. et al. (1994) Biochemistry 33:3632.
Entrez Gene IDs
3053 (Human); 15160 (Mouse)
Alternate Names
HC2; HC2LS2; HCF2; HCF2clade D (heparin cofactor), member 1; HC-II; Heparin Cofactor II; HLS2HCII; leuserpin 2; LS2; Protease inhibitor leuserpin-2; Serpin D1; serpin peptidase inhibitor, clade D (heparin cofactor), member 1

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse Serpin D1/Heparin Cofactor II Antibody

There are currently no reviews for this product. Be the first to review Mouse Serpin D1/Heparin Cofactor II Antibody and earn rewards!

Have you used Mouse Serpin D1/Heparin Cofactor II Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review