Mouse Serum Amyloid A4 Antibody
Mouse Serum Amyloid A4 Antibody Summary
Accession # P31532
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Serum Amyloid A4 in Mouse Brain (Cerebellum). Serum Amyloid A4 was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Mouse Serum Amyloid A4 Monoclonal Antibody (Catalog # MAB4276) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serum Amyloid A4
Mouse Serum Amyloid A4 (SAA4; also SAA5) is a 14 kDa member of the SAA family of proteins. It is a constitutively produced apolipoprotein component of HDL that may mediate HDL-VLDL interactions. Mature mouse SAA4 is 112 amino acids (aa) in length. Although human SAA4 is glycosylated, mouse SAA4 is not. Human SAA4 also shows truncation of its Lys-Lys-Tyr C-terminus. The mouse C-terminus is distantly related with a Glu-Lys-Phe tripeptide, but it is unknown if it is proteolytically processed. Over aa 19-130, mouse SAA4 shares 57% and 85% aa identity with mature human and rat SAA4, respectively.
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