Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 398104
View all Serum Amyloid A4 Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant mouse Serum Amyloid A4
Asp19-Phe130
Accession # P31532

Specificity

Detects mouse Serum Amyloid A4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Serum Amyloid A1, rhSerum Amyloid A4, or recombinant mouse Serum Amyloid A1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Serum Amyloid A4 Antibody

Serum Amyloid A4 antibody in Mouse Brain (Cerebellum) by Immunohistochemistry (IHC-Fr).

Serum Amyloid A4 in Mouse Brain (Cerebellum).

Serum Amyloid A4 was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Mouse Serum Amyloid A4 Monoclonal Antibody (Catalog # MAB4276) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Serum Amyloid A4 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (cerebellum)

Reviewed Applications

Read 1 review rated 5 using MAB4276 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serum Amyloid A4

Mouse Serum Amyloid A4 (SAA4; also SAA5) is a 14 kDa member of the SAA family of proteins.  It is a constitutively produced apolipoprotein component of HDL that may mediate HDL-VLDL interactions.  Mature mouse SAA4 is 112 amino acids (aa) in length.  Although human SAA4 is glycosylated, mouse SAA4 is not.  Human SAA4 also shows truncation of its Lys-Lys-Tyr C-terminus. The mouse C-terminus is distantly related with a Glu-Lys-Phe tripeptide, but it is unknown if it is proteolytically processed. Over aa 19-130, mouse SAA4 shares 57% and 85% aa identity with mature human and rat SAA4, respectively.   

Alternate Names

C-SAA, SAA4, SAA5

Entrez Gene IDs

6291 (Human); 20211 (Mouse); 365245 (Rat)

Gene Symbol

SAA4

UniProt

P31532

Additional Serum Amyloid A4 Products

Product Documents for Mouse Serum Amyloid A4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Serum Amyloid A4 Antibody

For research use only

Customer Reviews for Mouse Serum Amyloid A4 Antibody (1)

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  • Mouse Serum Amyloid A4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Mouse
    Verified Customer | Posted 12/18/2021
    Mouse Serum Amyloid A4 Antibody MAB4276

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Protocols

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