Detects mouse Serum Amyloid A4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human (rh) Serum Amyloid A1, rhSerum Amyloid A4, or recombinant mouse Serum
Amyloid A1 is observed.
Monoclonal Rat IgG2A Clone # 398104
Protein A or G purified from hybridoma culture supernatant
Serum Amyloid A4 in Mouse Brain (Cerebellum).
Serum Amyloid A4 was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Mouse Serum Amyloid A4 Monoclonal Antibody (Catalog # MAB4276) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serum Amyloid A4
Mouse Serum Amyloid A4 (SAA4; also SAA5) is a 14
kDa member of the SAA family of proteins.
It is a constitutively produced apolipoprotein component of HDL that may
mediate HDL-VLDL interactions. Mature
mouse SAA4 is 112 amino acids (aa) in length. Although human SAA4 is glycosylated, mouse
SAA4 is not. Human SAA4 also shows
truncation of its Lys-Lys-Tyr C-terminus. The mouse C-terminus is distantly related with a Glu-Lys-Phe tripeptide,
but it is unknown if it is proteolytically processed. Over aa 19-130, mouse
SAA4 shares 57% and 85% aa identity with mature human and rat SAA4,
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