Mouse Sonic Hedgehog/Shh C-Terminus Antibody

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Mouse Sonic Hedgehog/Shh C-Terminus Antibody Summary

Species Reactivity
Detects recombinant mouse Shh C-terminal peptide in direct ELISAs and Western blots. Does not cross-react in direct ELISAs with rmDhh C- or N-terminal peptides, rmIhh C- or N-terminal peptides, rhShh N-terminal peptide, or rmShh N-terminal peptide.
Monoclonal Rat IgG2A Clone # 55626
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse Sonic Hedgehog
Accession # Q62226.1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Mouse Sonic Hedgehog C-Terminus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sonic Hedgehog/Shh

Sonic Hedgehog (Shh) is a hedgehog protein that is instrumental in patterning the early embryo. It is cleaved by autoproteolysis to release an N-terminal signaling peptide that binds the multicomponent receptor complex containing the multi-pass transmembrane proteins Patched and Smoothened. The C-terminal peptide carries the protease domain which acts only in CIS. It also acts on a cholesterol transferase that covalently transfers the cholesterol molecule to the C-terminus of Shh-N.

Entrez Gene IDs
6469 (Human); 20423 (Mouse)
Alternate Names
HHG1; HHG-1; HLP3; HPE3; MCOPCB5; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; ShhNC; SMMCI; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS

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