Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 283910
View all Stella/Dppa3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Stella/Dppa3
Met1-Asn150
Accession # Q8QZY3

Specificity

Detects mouse Stella/Dppa3 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Stella/Dppa3 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Mouse Stella/Dppa3

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Stella/Dppa3

Stella, also known as Pgc7 or Dppa3, is expressed in primordial germ cells at the time of their emergence in gastrulating embryos. Stella may be an important maternal factor in the cleavage stages of mouse embryogenesis and is required for germ cell specification.

Long Name

Developmental Pluripotency-associated Protein 3

Alternate Names

Dppa3

Entrez Gene IDs

359787 (Human); 73708 (Mouse)

Gene Symbol

DPPA3

UniProt

Q8QZY3

Additional Stella/Dppa3 Products

Product Documents for Mouse Stella/Dppa3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Stella/Dppa3 Antibody

For research use only

Related Research Areas

Primordial and Mature Germ Cells

Citations for Mouse Stella/Dppa3 Antibody

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Protocols

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FAQs

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