Mouse TGF-beta 1 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY1679
DY1679-05
Ancillary Products Available
Mouse TGF-beta 1 ELISA Standard Curve
1 Image
Product Details
Procedure
Citations (87)
FAQs
Supplemental Products
Reviews (8)

Mouse TGF-beta 1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant TGF-ß1. The Reagent Diluent recommended may be suitable for most cell culture supernate, serum, and plasma samples. The Reagent Diluent selected for use can alter the performance of an immunoassay. Reagent Diluent optimization for samples with complex matrices such as serum and plasma, may improve their performance in this assay.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 1 (5 plates): (Catalog # DY007) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 1.

The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4


Reagent Diluent: (Catalog # DY997), or 1.4% delipidized bovine serum, 0.05% Tween 20 in PBS, pH 7.2-7.4, 0.2 μm filtered.

Blocking Buffer: (Catalog # DY004), or 5% Tween 20 in PBS, pH 7.2-7.4, 0.2 μm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Activation Reagents Required:

Sample Activation Kit 1: 3 vials (10 mL/vial) of 1N HCL and 3 vials (10 mL/vial) of 1.2 N NaOH/1M HEPES (R&D Systems, Catalog # DY010).

 

Data Example

Mouse TGF-beta 1 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TGF-beta 1

Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.

TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic 'cysteine knot'. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts.

Long Name:
Transforming Growth Factor beta 1
Entrez Gene IDs:
7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
Alternate Names:
CEDLAP; DPD1; latency-associated peptide; TGF beta; TGF beta1; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1

Assay Procedure

ACTIVATION REAGENT PREPARATION

To activate latent TGF-ß1 to the immunoreactive form, prepare the following solutions for acid activation and neutralization. The solutions may be stored in polypropylene bottles at room temperature for up to one month.

Caution: Wear protective clothing and safety glasses during preparation or use of these reagents. Refer to the appropriate MSDS before use.

1 N HCl (100 mL) - To 91.67 mL of deionized water, slowly add 8.33 mL of 12 N HCl. Mix well.

1.2 N NaOH/0.5 M HEPES (100 mL) - To 75 mL of deionized water, slowly add 12 mL of 10 N NaOH. Mix well. Add 11.9 g of HEPES. Mix well. Bring final volume to 100 mL with deionized water.

TGF-β1 SAMPLE ACTIVATION

To activate latent TGF-β1 to immunoreactive TGF-β1, follow the activation procedure outlined below. Assay samples after neutralization (pH 7.2-7.6). Use polypropylene test tubes.

Note: Do not activate the kit standards. The kit standards contain active recombinant TGF-β1.

Cell Culture Supernates Serum/Plasma
To 100 μL of cell culture supernate, add 20 μL of 1 N HCI. To 40 μL of serum/plasma, add 10 μL of 1 N HCI.
Mix well. Mix well.
Incubate 10 minutes at room temperature. Incubate 10 minutes at room temperature.
Neutralize the acidified sample by adding 20 μL of 1.2 N NaOH/0.5 M HEPES. Neutralize the acidified sample by adding 10 μL of 1.2 N NaOH/0.5 M HEPES.
Mix well. Mix well.
Assay immediately. Prior to the assay, dilute the activated sample 60-fold with Reagent Diluent.*
The concentration read off the standard curve must be multiplied by the dilution factor, 1.4. The concentration read off the standard curve must be multiplied by the appropriate dilution factor, 90.

 

*A suggested 60-fold dilution is 10 μL of activated sample + 590 μL of Reagent Diluent.

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody (to the working concentration stated in the product datasheet ) in PBS without carrier protein. Immediately coat a 96-well microplate with 100 µL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 µL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block each well of the microplate as recommended in the product datasheet. Incubate at room temperature for a minimum of 1 hour.

    Note: The recommended Reagent Diluent typically contains 1% BSA. Some DuoSet Development Kits require alternative blocking agents, or for plates to be blocked overnight with a higher percentage of BSA, please see the product datasheet for details.
     
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

 

PRECAUTION
The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.

Assay Procedure

  1. Add 100 µL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 µL of the Detection Antibody, diluted in Reagent Diluent (as recommended in the product datasheet), to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 µL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 µL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 µL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse TGF-beta 1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

87 Citations: Showing 1 - 10
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  1. Opisthorchis viverrini antigens up-regulates the expression of CD80 and MHC class II in JAWSII mouse dendritic cells and promotes IL-10 and TGF-&beta secretions
    Authors: S Jittimanee, S Wongratana, C Kaewraemru, J Jittimanee
    Parasitology international, 2021;84(0):102401.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Physalis angulata reduces the progression of chronic experimental periodontitis by immunomodulatory mechanisms
    Authors: PS Vieceli, PJ Lima Juiz, PS Sales Laur, RD Couto, TC Barbosa To, IM Ribeiro, MB Pereira So, CF Villarreal
    Journal of ethnopharmacology, 2021;0(0):113986.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Glutaredoxin deficiency promotes activation of the transforming growth factor beta pathway in airway epithelial cells, in association with fibrotic airway remodeling
    Authors: SB Chia, JD Nolin, R Aboushoush, C Erikson, CG Irvin, ME Poynter, J van der Ve, DJ Taatjes, A van der Vl, V Anathy, YMW Janssen-He
    Redox Biol, 2020;37(0):101720.
    Species: Mouse
    Sample Types: BALF
  4. Th17/Treg imbalance in COPD development: suppressors of cytokine signaling and signal transducers and activators of transcription proteins
    Authors: LEF Silva, JD Lourenço, KR Silva, FPR Santana, JB Kohler, AR Moreira, APP Velosa, CM Prado, RP Vieira, MV Aun, IFLC Tibério, JT Ito, FDTQS Lopes
    Scientific Reports, 2020;10(1):15287.
    Species: Mouse
    Sample Types: Tissue Homogenates
  5. Spermidine attenuates bleomycin-induced lung fibrosis by inducing autophagy and inhibiting endoplasmic reticulum stress (ERS)-induced cell death in mice
    Authors: AR Baek, J Hong, KS Song, AS Jang, DJ Kim, SS Chin, SW Park
    Experimental & Molecular Medicine, 2020;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  6. Myeloid cell-derived TGF-beta signaling regulates ECM deposition in mammary carcinoma via adenosine-dependent mechanisms
    Authors: G Vasiukov, T Novitskaya, A Zijlstra, P Owens, F Ye, Z Zhao, HL Moses, T Blackwell, I Feoktistov, SV Novitskiy
    Cancer Res., 2020;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Temporal and spatial modulation of the tumor and systemic immune response in the murine Gl261 glioma model
    Authors: KJ McKelvey, AL Hudson, R Prasanna K, JS Wilmott, GH Attrill, GV Long, RA Scolyer, SJ Clarke, HR Wheeler, CI Diakos, VM Howell
    PLoS ONE, 2020;15(4):e0226444.
    Species: Mouse
    Sample Types: Plasma
  8. An Adrenalectomy Mouse Model Reflecting Clinical Features for Chronic Fatigue Syndrome
    Authors: JS Lee, YJ Jeon, SY Park, CG Son
    Biomolecules, 2020;10(1):.
    Species: Mouse
    Sample Types: Serum
  9. Role of adipose tissue inflammation in fat pad loss induced by fasting in lean and mildly obese mice
    Authors: DR Lacerda, KA Costa, ALM Silveira, DF Rodrigues, AN Silva, JL Sabino, V Pinho, GB Menezes, DD Soares, MM Teixeira, AVM Ferreira
    J. Nutr. Biochem., 2019;72(0):108208.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. NLRX1 does not play a role in diabetes nor the development of diabetic nephropathy induced by multiple low doses of streptozotocin
    Authors: AML Scantleber, M Uil, LM Butter, R Poelman, N Claessen, SE Girardin, S Florquin, JJTH Roelofs, JC Leemans
    PLoS ONE, 2019;14(3):e0214437.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. In-depth characterization of congenital Zika syndrome in immunocompetent mice: Antibody-dependent enhancement and an antiviral peptide therapy
    Authors: VN Camargos, G Foureaux, DC Medeiros, VT da Silveir, CM Queiroz-Ju, ALB Matosinhos, AFA Figueiredo, CDF Sousa, TP Moreira, VF Queiroz, ACF Dias, KTO Santana, I Passos, ALCV Real, LC Silva, FAG Mourão, NT Wnuk, MAP Oliveira, S Macari, T Silva, GP Garlet, JA Jackman, FM Soriani, MFD Moraes, EMAM Mendes, FM Ribeiro, GMJ Costa, AL Teixeira, NJ Cho, ACP Oliveira, MM Teixeira, VV Costa, DG Souza
    EBioMedicine, 2019;0(0):.
    Species: Mouse
    Sample Types: Plasma
  12. Cellular immune response against NTHi infecting the pre-inflamed middle ear of the Junbo mouse
    Authors: PP Vikhe, T Purnell, SDM Brown, DW Hood
    Infect. Immun., 2019;0(0):.
    Species: Mouse
    Sample Types: Middle Ear Fluid
  13. Smad7 Controls Immunoregulatory PDL2/1-PD1 Signaling in Intestinal Inflammation and Autoimmunity
    Authors: LP Garo, AK Ajay, M Fujiwara, V Beynon, C Kuhn, G Gabriely, S Sadhukan, R Raheja, S Rubino, HL Weiner, G Murugaiyan
    Cell Rep, 2019;28(13):3353-3366.e5.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. TGFbeta-induced degradation of TRAF3 in mesenchymal progenitor cells causes age-related osteoporosis
    Authors: J Li, A Ayoub, Y Xiu, X Yin, JO Sanders, A Mesfin, L Xing, Z Yao, BF Boyce
    Nat Commun, 2019;10(1):2795.
    Species: Mouse
    Sample Types: Serum
  15. Maternal Obesity in Mice Exacerbates the Allergic Inflammatory Response in the Airways of Male Offspring
    Authors: RR E-Lacerda, CJ Teixeira, S Bordin, E Antunes, GF Anhê
    Nutrients, 2019;11(12):.
    Species: Mouse
    Sample Types: BALF
  16. Strongyloides venezuelensis-infection alters the profile of cytokines and liver inflammation in mice co-infected with Schistosoma mansoni
    Authors: MC de Rezende, JMP Moreira, LLM Fernandes, VF Rodrigues, D Negrão-Cor
    Cytokine, 2019;127(0):154931.
    Species: Mouse
    Sample Types: Tissue Homogenates
  17. Roxatidine inhibits fibrosis by inhibiting NF?kappaB and MAPK signaling in macrophages sensing breast implant surface materials
    Authors: L Ji, T Wang, L Tian, H Song, M Gao
    Mol Med Rep, 2019;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. TRAIL-Dependent Resolution of Pulmonary Fibrosis
    Authors: DM Habiel, AP Moreira, UB Ismailoglu, MP Dunleavy, KA Cavassani, N van Rooije, AL Coelho, CM Hogaboam
    Mediators Inflamm., 2018;2018(0):7934362.
    Species: Mouse
    Sample Types: Tissue Homogenates
  19. Neuronal Transforming Growth Factor beta Signaling via SMAD3 Contributes to Pain in Animal Models of Chronic Pancreatitis
    Authors: L Liu, Y Zhu, M Noë, Q Li, PJ Pasricha
    Gastroenterology, 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  20. Identification of periplakin as a major regulator of lung injury and repair in mice
    Authors: V Besnard, R Dagher, T Madjer, A Joannes, M Jaillet, M Kolb, P Bonniaud, LA Murray, MA Sleeman, B Crestani
    JCI Insight, 2018;3(5):.
    Species: Mouse
    Sample Types: BALF
  21. Candesartan ameliorates brain inflammation associated with Alzheimer's disease
    Authors: N Torika, K Asraf, RN Apte, S Fleisher-B
    CNS Neurosci Ther, 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  22. Lactobacillus fermentum HP3-Mediated Fermented Hericium erinaceus Juice as a Health Promoting Food Supplement to Manage Diabetes Mellitus
    Authors: C Chaiyasut, S Woraharn, BS Sivamaruth, N Lailerd, P Kesika, S Peerajan
    J Evid Based Integr Med, 2018;23(0):2515690X18765.
    Species: Rat
    Sample Types: Serum
  23. IGF-1-Overexpressing Mesenchymal Stem/Stromal Cells Promote Immunomodulatory and Proregenerative Effects in Chronic Experimental Chagas Disease
    Authors: DN Silva, BSF Souza, CM Azevedo, JF Vasconcelo, PG de Jesus, MS Feitoza, CS Meira, GB Carvalho, BR Cavalcante, R Ribeiro-Do, MBP Soares
    Stem Cells Int, 2018;2018(0):9108681.
    Species: Mouse
    Sample Types: Serum
  24. Calcineurin-mediated IL-2 production by CD11chighMHCII+myeloid cells is crucial for intestinal immune homeostasis
    Authors: A Mencarelli, HJ Khameneh, J Fric, M Vacca, S El Daker, B Janela, JP Tang, S Nabti, A Balachande, TS Lim, F Ginhoux, P Ricciardi-, A Mortellaro
    Nat Commun, 2018;9(1):1102.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. Inhibition of profibrotic microRNA-21 affects platelets and their releasate
    Authors: T Barwari, S Eminaga, U Mayr, R Lu, PC Armstrong, MV Chan, M Sahraei, M Fernández-, T Moreau, J Barallobre, M Lynch, X Yin, C Schulte, F Baig, R Pechlaner, SR Langley, A Zampetaki, P Santer, M Weger, R Plasenzott, M Schosserer, J Grillari, S Kiechl, J Willeit, AM Shah, C Ghevaert, TD Warner, C Fernández-, Y Suárez, M Mayr
    JCI Insight, 2018;3(21):.
    Species: Mouse
    Sample Types: Plasma
  26. Role of integrin alpha8 in murine model of lung fibrosis
    Authors: CF Hung, CL Wilson, YH Chow, LM Schnapp
    PLoS ONE, 2018;13(5):e0197937.
    Species: Mouse
    Sample Types: BALF
  27. miR-130a and miR-145 reprogram Gr-1+CD11b+ myeloid cells and inhibit tumor metastasis through improved host immunity
    Authors: H Ishii, SK Vodnala, BR Achyut, JY So, MC Hollander, TF Greten, A Lal, L Yang
    Nat Commun, 2018;9(1):2611.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  28. Activation of M1 macrophages play a critical role in the initiation of acute lung injury
    Authors: HL Lu, XY Huang, YF Luo, WP Tan, PF Chen, YB Guo
    Biosci. Rep., 2018;0(0):.
    Species: Mouse
    Sample Types: Serum
  29. Ileocolic resection is associated with increased susceptibility to injury in a murine model of colitis
    Authors: T Perry, M Laffin, RN Fedorak, A Thiesen, B Dicken, KL Madsen
    PLoS ONE, 2017;12(9):e0184660.
    Species: Mouse
    Sample Types: Tissue Homogenates
  30. Distinctive role of efferocytosis in dendritic cell maturation and migration in sterile or infectious conditions
    Authors: L de Aquino, NN Dejani, FF Verdan, AB Orlando, V Ninõ, F De Nuzzi D, AC Salina, AI Medeiros
    Immunology, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  31. Somatic extracts of Marshallagia marshalli downregulate the Th2 associated immune responses in ovalbumin-induced airway inflammation in BALB/c mice
    Authors: S Parande Sh, A Ebrahimby, A Dousty, M Maleki, A Movassaghi, H Borji, A Haghparast
    Parasit Vectors, 2017;10(1):233.
    Species: Mouse
    Sample Types: BALF
  32. HIV protease inhibitor-induced cardiac dysfunction and fibrosis is mediated by platelet-derived TGF-?1 and can be suppressed by exogenous carbon monoxide
    Authors: J Laurence, S Elhadad, T Robison, H Terry, R Varshney, S Woolington, S Ghafoory, ME Choi, J Ahamed
    PLoS ONE, 2017;12(10):e0187185.
    Species: Mouse
    Sample Types: Plasma
  33. Hypercholesterolemia Induces Differentiation of Regulatory T Cells in the Liver
    Authors: RK Mailer, A Gisterå, KA Polyzos, DF Ketelhuth, GK Hansson
    Circ. Res., 2017;0(0):.
    Species: Mouse
    Sample Types: Plasma
  34. IRF-8 regulates expansion of myeloid-derived suppressor cells and Foxp3+ regulatory T cells and modulates Th2 immune responses to gastrointestinal nematode infection
    Authors: RM Valanparam, M Tam, PP Gros, JP Auger, M Segura, P Gros, A Jardim, TG Geary, K Ozato, MM Stevenson
    PLoS Pathog., 2017;13(10):e1006647.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  35. Overexpression of OSM and IL-6 impacts the polarization of pro-fibrotic macrophages and the development of bleomycin-induced lung fibrosis
    Authors: EA Ayaub, A Dubey, J Imani, F Botelho, MRJ Kolb, CD Richards, K Ask
    Sci Rep, 2017;7(1):13281.
    Species: Mouse
    Sample Types: BALF
  36. Brazilian red propolis improves cutaneous wound healing suppressing inflammation-associated transcription factor NF?B
    Authors: Flavia Regina Sobreira Corrêa
    Biomed. Pharmacother, 2016;86(0):162-171.
    Species: Mouse
    Sample Types: Plasma
  37. Induced regulatory T cells are phenotypically unstable and do not protect mice from rapidly progressive glomerulonephritis
    Immunology, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  38. Polarization of macrophages towards M2 phenotype is favored by reduction in iPLA2?
    J Biol Chem, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  39. Regulation of eosinophilia and allergic airway inflammation by the glycan-binding protein galectin-1
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Types: BALF
  40. The phytogestrogenic stilbenes, arachidin-1 and resveratrol, modulate regulatory T�cell functions responsible for successful aging in aged ICR mice
    Authors: Robin Yih-Yuan Chiou
    Int. J. Mol. Med., 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  41. Neuronal Protein 3.1 Deficiency Leads to Reduced Cutaneous Scar Collagen Deposition and Tensile Strength due to Impaired Transforming Growth Factor-?1 to -?3 Translation
    Authors: Tao Cheng
    Am. J. Pathol, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
  42. Induction of Siglec-1 by Endotoxin Tolerance Suppresses the Innate Immune Response by Promoting TGF-?1 Production
    Authors: Y Wu, C Lan, D Ren, GY Chen
    J. Biol. Chem., 2016;291(23):12370-82.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  43. Gene Therapy Induces Antigen-Specific Tolerance in Experimental Collagen-Induced Arthritis
    PLoS ONE, 2016;11(5):e0154630.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  44. High endogenous activated protein C levels attenuates bleomycin-induced pulmonary fibrosis
    Authors: Cong Lin
    J Cell Mol Med, 2016;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  45. Proinflammatory M1 macrophages inhibit RANKL-induced osteoclastogenesis
    Infect Immun, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  46. Protease activated receptor-1 regulates macrophage-mediated cellular senescence: a risk for idiopathic pulmonary fibrosis
    Authors: C Lin, F Rezaee, M Waasdorp, K Shi, T van der Po, K Borensztaj, CA Spek
    Oncotarget, 2015;6(34):35304-14.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  47. Differential Immunometabolic Phenotype in Th1 and Th2 Dominant Mouse Strains in Response to High-Fat Feeding.
    Authors: Jovicic N, Jeftic I, Jovanovic I, Radosavljevic G, Arsenijevic N, Lukic M, Pejnovic N
    PLoS ONE, 2015;10(7):e0134089.
    Species: Mouse
    Sample Types: Tissue Homogenates
  48. Influenza A virus-dependent remodeling of pulmonary clock function in a mouse model of COPD.
    Authors: Sundar, Isaac K, Ahmad, Tanveer, Yao, Hongwei, Hwang, Jae-woon, Gerloff, Janice, Lawrence, B Paige, Sellix, Michael, Rahman, Irfan
    Sci Rep, 2015;4(0):9927.
    Species: Mouse
    Sample Types: BALF
  49. Intestinal helminths regulate lethal acute graft-versus-host disease and preserve the graft-versus-tumor effect in mice.
    Authors: Li Y, Chen H, Bannick N, Henry M, Holm A, Metwali A, Urban J, Rothman P, Weiner G, Blazar B, Elliott D, Ince M
    J Immunol, 2015;194(3):1011-20.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  50. IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis.
    Authors: Coffelt S, Kersten K, Doornebal C, Weiden J, Vrijland K, Hau C, Verstegen N, Ciampricotti M, Hawinkels L, Jonkers J, de Visser K
    Nature, 2015;522(7556):345-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
  51. Dichloroacetate modulates cytokines toward T helper 1 function via induction of the interleukin-12-interferon-gamma pathway.
    Authors: Badr M, Qinna N, Qadan F, Matalka K
    Onco Targets Ther, 2014;7(0):193-201.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  52. The glycosylated Rv1860 protein of mycobacterium tuberculosis inhibits dendritic cell mediated TH1 and TH17 polarization of T cells and abrogates protective immunity conferred by BCG.
    Authors: Satchidanandam V, Kumar N, Jumani R, Challu V, Elangovan S, Khan N
    PLoS Pathog, 2014;10(6):e1004176.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  53. Unique macrophages different from M1/M2 macrophages inhibit T cell mitogenesis while upregulating Th17 polarization.
    Authors: Tatano Y, Shimizu T, Tomioka H
    Sci Rep, 2014;4(0):4146.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  54. Genetic suppression of inflammation blocks the tumor-promoting effects of TGF-beta in gastric tissue.
    Authors: Ota M, Horiguchi M, Fang V, Shibahara K, Kadota K, Loomis C, Cammer M, Rifkin D
    Cancer Res, 2014;74(9):2642-51.
    Species: Mouse
    Sample Types: Serum
  55. The monocyte to macrophage transition in the murine sterile wound.
    Authors: Crane, Meredith, Daley, Jean M, van Houtte, Olivier, Brancato, Samielle, Henry, William, Albina, Jorge E
    PLoS ONE, 2014;9(1):e86660.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  56. Mesenchymal stem cells recruited by active TGFbeta contribute to osteogenic vascular calcification.
    Authors: Wang W, Li C, Pang L, Shi C, Guo F, Chen A, Cao X, Wan M
    Stem Cells Dev, 2014;23(12):1392-404.
    Species: Mouse
    Sample Types: Plasma
  57. Hyperactive transforming growth factor-beta1 signaling potentiates skeletal defects in a neurofibromatosis type 1 mouse model.
    Authors: Rhodes S, Wu X, He Y, Chen S, Yang H, Staser K, Wang J, Zhang P, Jiang C, Yokota H, Dong R, Peng X, Yang X, Murthy S, Azhar M, Mohammad K, Xu M, Guise T, Yang F
    J Bone Miner Res, 2013;28(12):2476-89.
    Species: Mouse
    Sample Types: Serum
  58. Mesenchymal stem cells inhibit cutaneous radiation-induced fibrosis by suppressing chronic inflammation.
    Authors: Horton J, Hudak K, Chung E, White A, Scroggins B, Burkeen J, Citrin D
    Stem Cells, 2013;31(10):2231-41.
    Species: Mouse
    Sample Types: Tissue Homogenates
  59. Toll-like Receptor 2 (TLR2), Transforming Growth Factor-beta, Hyaluronan (HA), and Receptor for HA-mediated Motility (RHAMM) Are Required for Surfactant Protein A-stimulated Macrophage Chemotaxis.
    Authors: Foley J, Lam D, Jiang H, Liao J, Cheong N, McDevitt T, Zaman A, Wright J, Savani R
    J Biol Chem, 2012;287(44):37406-19.
    Species: Human
    Sample Types: Cell Culture Supernates
  60. Role of Toll interleukin-1 receptor (IL-1R) 8, a negative regulator of IL-1R/Toll-like receptor signaling, in resistance to acute Pseudomonas aeruginosa lung infection.
    Authors: Veliz Rodriguez T, Moalli F, Polentarutti N, Paroni M, Bonavita E, Anselmo A, Nebuloni M, Mantero S, Jaillon S, Bragonzi A, Mantovani A, Riva F, Garlanda C
    Infect. Immun., 2012;80(1):100-9.
    Species: Mouse
    Sample Types: Serum
  61. The Therapeutic Potential of the Humoral Pattern Recognition Molecule PTX3 in Chronic Lung Infection Caused by Pseudomonas aeruginosa.
    Authors: Moalli F, Paroni M, Veliz Rodriguez T, Riva F, Polentarutti N, Bottazzi B, Valentino S, Mantero S, Nebuloni M, Mantovani A, Bragonzi A, Garlanda C
    J. Immunol., 2011;186(9):5425-34.
    Species: Mouse
    Sample Types: Tissue Homogenates
  62. High expression of IL-22 suppresses antigen-induced immune responses and eosinophilic airway inflammation via an IL-10-associated mechanism.
    Authors: Nakagome K, Imamura M, Kawahata K
    J. Immunol., 2011;187(10):5077-89.
    Species: Mouse
    Sample Types: BALF
  63. Phosphoinositide 3-kinase gamma plays a critical role in bleomycin-induced pulmonary inflammation and fibrosis in mice.
    Authors: Russo RC, Garcia CC, Barcelos LS, Rachid MA, Guabiraba R, Roffe E, Souza AL, Sousa LP, Mirolo M, Doni A, Cassali GD, Pinho V, Locati M, Teixeira MM
    J. Leukoc. Biol., 2011;89(2):269-82.
    Species: Mouse
    Sample Types: Tissue Homogenates
  64. T-cell polarization depends on concentration of the danger signal used to activate dendritic cells.
    Authors: Peters M, Dudziak K, Stiehm M
    Immunol. Cell Biol., 2010;88(5):537-44.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  65. Latent herpesvirus infection augments experimental pulmonary fibrosis.
    Authors: Vannella KM, Luckhardt TR, Wilke CA, van Dyk LF, Toews GB, Moore BB
    Am. J. Respir. Crit. Care Med., 2010;181(5):465-77.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  66. Th17 cells are the dominant T cell subtype primed by Shigella flexneri mediating protective immunity.
    Authors: Sellge G, Magalhaes JG, Konradt C, Fritz JH, Salgado-Pabon W, Eberl G, Bandeira A, Di Santo JP, Sansonetti PJ, Phalipon A
    J. Immunol., 2010;184(4):2076-85.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  67. A critical role for C5L2 in the pathogenesis of experimental allergic asthma.
    Authors: Zhang X, Schmudde I, Laumonnier Y, Pandey MK, Clark JR, Konig P, Gerard NP, Gerard C, Wills-Karp M, Kohl J
    J. Immunol., 2010;185(11):6741-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  68. Importance of TLR2 in the direct response of T lymphocytes to Schistosoma mansoni antigens.
    Authors: Burton OT, Gibbs S, Miller N, Jones FM, Wen L, Dunne DW, Cooke A, Zaccone P
    Eur. J. Immunol., 2010;40(8):2221-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  69. Mesenchymal stem cells produce Wnt isoforms and TGF-beta1 that mediate proliferation and procollagen expression by lung fibroblasts.
    Authors: Salazar KD, Lankford SM, Brody AR
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(5):L1002-11.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  70. BCL9 promotes tumor progression by conferring enhanced proliferative, metastatic, and angiogenic properties to cancer cells.
    Authors: Mani M, Carrasco DE, Zhang Y, Takada K, Gatt ME, Dutta-Simmons J, Ikeda H, Diaz-Griffero F, Pena-Cruz V, Bertagnolli M, Myeroff LL, Markowitz SD, Anderson KC, Carrasco DR
    Cancer Res., 2009;69(19):7577-86.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  71. Serotonin and angiotensin receptors in cardiac fibroblasts coregulate adrenergic-dependent cardiac hypertrophy.
    Authors: Jaffre F, Bonnin P, Callebert J, Debbabi H, Setola V, Doly S, Monassier L, Mettauer B, Blaxall BC, Launay JM, Maroteaux L
    Circ. Res., 2009;104(1):113-23.
    Species: Mouse
    Sample Types: Plasma
  72. Hypoxia-induced alveolar epithelial-mesenchymal transition requires mitochondrial ROS and hypoxia-inducible factor 1.
    Authors: Zhou G, Dada LA, Wu M, Kelly A, Trejo H, Zhou Q, Varga J, Sznajder JI
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(6):L1120-30.
    Species: Rat
    Sample Types: Cell Culture Supernates
  73. CD69+ CD4+ CD25- T cells, a new subset of regulatory T cells, suppress T cell proliferation through membrane-bound TGF-beta 1.
    Authors: Han Y, Guo Q, Zhang M, Chen Z, Cao X
    J. Immunol., 2009;182(1):111-20.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  74. Mucosal immunity in mice induced by orally administered transgenic rice.
    Authors: Zhang X, Yuan Z, Duan Q, Zhu H, Yu H, Wang Q
    Vaccine, 2009;27(10):1596-600.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  75. Lack of Toll IL-1R8 exacerbates Th17 cell responses in fungal infection.
    Authors: Bozza S, Zelante T, Moretti S, Bonifazi P, DeLuca A, D'Angelo C, Giovannini G, Garlanda C, Boon L, Bistoni F, Puccetti P, Mantovani A, Romani L
    J. Immunol., 2008;180(6):4022-31.
    Species: Mouse
    Sample Types: Tissue Homogenates
  76. In vivo investigations on anti-fibrotic potential of proteasome inhibition in lung and skin fibrosis.
    Authors: Fineschi S, Bongiovanni M, Donati Y, Djaafar S, Naso F, Goffin L, Argiroffo CB, Pache JC, Dayer JM, Ferrari-Lacraz S, Chizzolini C
    Am. J. Respir. Cell Mol. Biol., 2008;39(4):458-65.
    Species: Mouse
    Sample Types: BALF
  77. Methylprednisolone improves lung mechanics and reduces the inflammatory response in pulmonary but not in extrapulmonary mild acute lung injury in mice.
    Authors: Leite-Junior JH, Garcia CS, Souza-Fernandes AB, Silva PL, Ornellas DS, Larangeira AP, Castro-Faria-Neto HC, Morales MM, Negri EM, Capelozzi VL, Zin WA, Pelosi P, Bozza PT, Rocco PR
    Crit. Care Med., 2008;36(9):2621-8.
    Species: Mouse
    Sample Types: BALF
  78. Coexpression of TGF-beta1 and IL-10 enables regulatory T cells to completely suppress airway hyperreactivity.
    Authors: Presser K, Schwinge D, Wegmann M, Huber S, Schmitt S, Quaas A, Maxeiner JH, Finotto S, Lohse AW, Blessing M, Schramm C
    J. Immunol., 2008;181(11):7751-8.
    Species: Mouse
    Sample Types: BALF
  79. Expression of inflammatory mediators in the otitis media induced by Helicobacter pylori antigen in mice.
    Authors: Kariya S, Okano M, Fukushima K, Nomiya S, Kataoka Y, Nomiya R, Akagi H, Nishizaki K
    Clin. Exp. Immunol., 2008;154(1):134-40.
    Species: Mouse
    Sample Types: Middle Ear Lavage Fluid
  80. Transforming growth factor-beta regulates house dust mite-induced allergic airway inflammation but not airway remodeling.
    Authors: Fattouh R, Midence NG, Arias K, Johnson JR, Walker TD, Goncharova S, Souza KP, Gregory RC, Lonning S, Gauldie J, Jordana M
    Am. J. Respir. Crit. Care Med., 2008;177(6):593-603.
    Species: Mouse
    Sample Types: BALF
  81. CD40L disruption enhances Abeta vaccine-mediated reduction of cerebral amyloidosis while minimizing cerebral amyloid angiopathy and inflammation.
    Authors: Obregon D, Hou H, Bai Y, Nikolic WV, Mori T, Luo D, Zeng J, Ehrhart J, Fernandez F, Morgan D, Giunta B, Town T, Tan J
    Neurobiol. Dis., 2007;29(2):336-53.
    Species: Mouse
    Sample Types: Plasma
  82. Expansion of CD4+CD25+ regulatory T cells by intravenous immunoglobulin: a critical factor in controlling experimental autoimmune encephalomyelitis.
    Authors: Ephrem A, Chamat S, Miquel C, Fisson S, Mouthon L, Caligiuri G, Delignat S, Elluru S, Bayry J, Lacroix-Desmazes S, Cohen JL, Salomon BL, Kazatchkine MD, Kaveri SV, Misra N
    Blood, 2007;111(2):715-22.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  83. Poly(ADP-ribose) polymerase-1 (PARP-1) controls lung cell proliferation and repair after hyperoxia-induced lung damage.
    Authors: Pagano A, Metrailler-Ruchonnet I, Aurrand-Lions M, Lucattelli M, Donati Y, Argiroffo CB
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(3):L619-29.
    Species: Mouse
    Sample Types: BALF
  84. Nod1-mediated innate immune recognition of peptidoglycan contributes to the onset of adaptive immunity.
    Authors: Fritz JH, Le Bourhis L, Sellge G, Magalhaes JG, Fsihi H, Kufer TA, Collins C, Viala J, Ferrero RL, Girardin SE, Philpott DJ
    Immunity, 2007;26(4):445-59.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  85. Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
    Authors: Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE
    Arthritis Rheum., 2006;54(8):2402-14.
    Species: Rat
    Sample Types: Tissue Homogenates
  86. Induction of prolonged infiltration of T lymphocytes and transient T lymphocyte-dependent collagen deposition in mouse lungs following adenoviral gene transfer of CCL18.
    Authors: Luzina IG, Papadimitriou JC, Anderson R, Pochetuhen K, Atamas SP
    Arthritis Rheum., 2006;54(8):2643-55.
    Species: Mouse
    Sample Types: BALF
  87. Hierarchical suppression of asthma-like responses by mucosal tolerance.
    Authors: Keller AC, Mucida D, Gomes E, Faquim-Mauro E, Faria AM, Rodriguez D, Russo M
    J. Allergy Clin. Immunol., 2006;117(2):283-90.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Mouse TGF-beta 1 DuoSet ELISA
By Anonymous on 03/29/2021
Application: Sample Tested: Serum and Plasma

we used this kit to quantify Mouse TGF beta 1 mouse sera, it produces a very good standard curve, levels of TGF beta in control B6 mice is very low.


Mouse TGF-beta 1 DuoSet ELISA
By Anonymous on 02/04/2021
Application: Sample Tested: Dendritic cells

TGF-1 in cell culture supernatants was measured after converting latent TGF-1 to active TGF-1 by acidification (10-min incubation at room temperature with 0.2 volume of 1 N HCl for cell culture supernatants, followed by neutralization by adding the same volume
of 1.2 N NaOH in 0.5 M HEPES) with a ELISA assay.


Mouse TGF-beta 1 DuoSet ELISA
By Anonymous on 10/28/2020
Application: Sample Tested: Adult pancreas

Mouse TGF-beta 1 DuoSet ELISA
By Anonymous on 11/15/2019
Application: Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line

Mouse TGF-beta 1 DuoSet ELISA
By Anonymous on 05/30/2019
Application: Sample Tested: Serum and Plasma

Followed the kit directions for Serum/Plasma TGF-b activation. Our serum needed to be diluted due to the high BCA levels. We were able to see a difference on our samples and the standard curve looked great.


Mouse TGF-beta 1 DuoSet ELISA
By Anonymous on 10/19/2017
Application: Sample Tested: Cell Culture Supernates

Mouse TGF-beta 1 DuoSet ELISA
By Anonymous on 05/02/2017
Application: Sample Tested: Lung tissue

Mouse TGF-beta 1 DuoSet ELISA
By Anonymous on 07/05/2016
Application: Sample Tested: Mouse BAL fluid