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Mouse TGF-beta 1 DuoSet ELISA

Name: Mouse TGF-beta 1 DuoSet ELISA
Brand: R&D Systems
SKU: DY1679
Price: 890 USD
Availability: InStock
Rating: 4.533333333333333 (15 reviews)

R&D Systems | Catalog # DY1679

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Product Summary
Product Specifications
Scientific Data
Kit Contents
Other Reagents Required
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

31.2-2000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Mouse

Mouse TGF-beta 1 DuoSet ELISA Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

View all TGF-beta 1 ELISA Kits »

Product Summary for Mouse TGF-beta 1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant TGF-ß1. The Reagent Diluent recommended may be suitable for most cell culture supernate, serum, and plasma samples. The Reagent Diluent selected for use can alter the performance of an immunoassay. Reagent Diluent optimization for samples with complex matrices such as serum and plasma, may improve their performance in this assay.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Mouse TGF-beta 1 DuoSet ELISA

Mouse TGF-beta 1 ELISA Standard Curve

Kit Contents for Mouse TGF-beta 1 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)

Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)

Microplates: (Catalog # DY990), or equivalent

Plate Sealers: (Catalog # DY992), or equivalent

*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TGF-beta 1

TGF-beta 1 (Transforming Growth Factor beta 1) is a pleiotrophic cytokine that regulates immune function, proliferation, and epithelial-mesenchymal transition. TGF-beta 1 associates with LAP to form a latent complex. It can be is activated from latency by plasmin, matrix metalloproteases, thrombospondin 1, and a subset of integrins. TGF-beta 1 signals through complexes of TGF-beta RII with TGF-beta RI/ALK-5 or ALK-1. TGF-beta signaling is modulated by the accessory receptors TGF-beta RIII/Betaglycan and Endoglin/CD105.

Long Name

Transforming Growth Factor beta 1

Alternate Names

TGF beta1, TGFB, TGFB1, TGFbeta 1

Entrez Gene IDs

7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)

Gene Symbol

TGFB1

Additional TGF-beta 1 Products

Product Documents for Mouse TGF-beta 1 DuoSet ELISA

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse TGF-beta 1 DuoSet ELISA

For research use only

Related Research Areas

Cancer Biomarkers

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Angiogenesis

Early Mesodermal Lineage Markers

Hypoxia Inducible Factors (HIFs)

Inflammatory Mediators

Microglia Activation Markers

Myeloid-derived Suppressor Cells (MDSC)

Preeclampsia

Regulatory T Cells (Tregs)

Citations for Mouse TGF-beta 1 DuoSet ELISA

Customer Reviews for Mouse TGF-beta 1 DuoSet ELISA (15)

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Mouse TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: Tissue Homogenates

Verified Customer | Posted 08/29/2023
Mouse TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: Lung tissue

Verified Customer | Posted 08/01/2023
Mouse TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: lung fluid

Verified Customer | Posted 05/05/2023
Mouse TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: lung homogenate

Verified Customer | Posted 09/16/2022

very economic and stable kit
Mouse TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: Cell Culture Media

Verified Customer | Posted 07/20/2022
Mouse TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: Serum and Plasma

Verified Customer | Posted 03/29/2021

we used this kit to quantify Mouse TGF beta 1 mouse sera, it produces a very good standard curve, levels of TGF beta in control B6 mice is very low.
Mouse TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: Dendritic cells

Verified Customer | Posted 02/04/2021

TGF-1 in cell culture supernatants was measured after converting latent TGF-1 to active TGF-1 by acidification (10-min incubation at room temperature with 0.2 volume of 1 N HCl for cell culture supernatants, followed by neutralization by adding the same volume of 1.2 N NaOH in 0.5 M HEPES) with a ELISA assay.
Mouse TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: Adult pancreas

Verified Customer | Posted 10/28/2020
Mouse TGF-beta 1 DuoSet ELISA

Name: Yan Wang

Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line

Verified Customer | Posted 11/15/2019
Mouse TGF-beta 1 DuoSet ELISA

Name: Joseph Moore

Sample Tested: Serum and Plasma

Verified Customer | Posted 05/30/2019

Followed the kit directions for Serum/Plasma TGF-b activation. Our serum needed to be diluted due to the high BCA levels. We were able to see a difference on our samples and the standard curve looked great.
Name: Anonymous

Sample Tested: mouse liver lysates

Verified Customer | Posted 04/05/2019
Name: Anonymous

Sample Tested: Serum

Verified Customer | Posted 04/01/2019

I tested mouse serum with acid activation using RnD System's sample activation kit. Serum was tested at 1:100 which produced OD values within the linear range of the standard curve.
Mouse TGF-beta 1 DuoSet ELISA

Name: Adam Guess

Sample Tested: Cell Culture Supernates

Verified Customer | Posted 10/19/2017
Mouse TGF-beta 1 DuoSet ELISA

Name: Anonymous

Sample Tested: Lung tissue

Verified Customer | Posted 05/02/2017
Mouse TGF-beta 1 DuoSet ELISA

Name: I Sundar

Sample Tested: Mouse BAL fluid

Verified Customer | Posted 07/05/2016

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Showing 1 - 5 of 15 reviews Showing All

Protocols

View specific protocols for Mouse TGF-beta 1 DuoSet ELISA (DY1679):

ACTIVATION REAGENT PREPARATION

To activate latent TGF-ß1 to the immunoreactive form, prepare the following solutions for acid activation and neutralization. The solutions may be stored in polypropylene bottles at room temperature for up to one month.

Caution: Wear protective clothing and safety glasses during preparation or use of these reagents. Refer to the appropriate MSDS before use.

1 N HCl (100 mL) - To 91.67 mL of deionized water, slowly add 8.33 mL of 12 N HCl. Mix well.

1.2 N NaOH/0.5 M HEPES (100 mL) - To 75 mL of deionized water, slowly add 12 mL of 10 N NaOH. Mix well. Add 11.9 g of HEPES. Mix well. Bring final volume to 100 mL with deionized water.

TGF-β1 SAMPLE ACTIVATION

To activate latent TGF-β1 to immunoreactive TGF-β1, follow the activation procedure outlined below. Assay samples after neutralization (pH 7.2-7.6). Use polypropylene test tubes.

Note: Do not activate the kit standards. The kit standards contain active recombinant TGF-β1.

Cell Culture Supernates	Serum/Plasma
To 100 μL of cell culture supernate, add 20 μL of 1 N HCI.	To 40 μL of serum/plasma, add 10 μL of 1 N HCI.
Mix well.	Mix well.
Incubate 10 minutes at room temperature.	Incubate 10 minutes at room temperature.
Neutralize the acidified sample by adding 20 μL of 1.2 N NaOH/0.5 M HEPES.	Neutralize the acidified sample by adding 10 μL of 1.2 N NaOH/0.5 M HEPES.
Mix well.	Mix well.
Assay immediately.	Prior to the assay, dilute the activated sample 60-fold with Reagent Diluent.*
The concentration read off the standard curve must be multiplied by the dilution factor, 1.4.	The concentration read off the standard curve must be multiplied by the appropriate dilution factor, 90.

*A suggested 60-fold dilution is 10 μL of activated sample + 590 μL of Reagent Diluent.

GENERAL ELISA PROTOCOL

Plate Preparation

Dilute the Capture Antibody (to the working concentration stated in the product datasheet ) in PBS without carrier protein. Immediately coat a 96-well microplate with 100 µL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 µL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
Block each well of the microplate as recommended in the product datasheet. Incubate at room temperature for a minimum of 1 hour.

Note: The recommended Reagent Diluent typically contains 1% BSA. Some DuoSet Development Kits require alternative blocking agents, or for plates to be blocked overnight with a higher percentage of BSA, please see the product datasheet for details.
Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

PRECAUTION
The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.

Assay Procedure

Add 100 µL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
Repeat the aspiration/wash as in step 2 of Plate Preparation.
Add 100 µL of the Detection Antibody, diluted in Reagent Diluent (as recommended in the product datasheet), to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
Repeat the aspiration/wash as in step 2 of Plate Preparation.
Add 100 µL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
Repeat the aspiration/wash as in step 2.
Add 100 µL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
Add 50 µL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.