Mouse TGF-beta  RI/ALK-5 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5871P-100
FAB5871P-025
Detection of TGF‑ beta  RI/ALK‑5 in Mouse Splenocytes by Flow Cytometry.
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Mouse TGF-beta  RI/ALK-5 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detect mouse TGF-beta RI/ALK-5 in flow cytometry. Although this clone detects human and mouse TGF-beta RI/ALK-5 in Western blots, positive results have not been observed on human TGF-beta RI/ALK-5 in flow cytometry. In direct ELISAs and Western blots, this antibody shows no cross-reactivity with rrMIS RII, rhTGF‑ beta  RII, rhTGF‑ beta  RIIb, or rhTGF‑ beta  RIII.
Source
Monoclonal Rat IgG2A Clone # 141231
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF‑ beta  RI/ALK-5
Ala21-Glu121
Accession # BAA05023
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TGF‑ beta  RI/ALK‑5 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of TGF‑ beta  RI/ALK‑5 in Mouse Splenocytes by Flow Cytometry. NSA Mouse splenocytes were stained with (A) Rat Anti-Mouse TGF‑ beta  RI/ALK‑5 PE‑conjugated Monoclonal Antibody (Catalog # FAB5871P) or Rat IgG2a isotype control antibody (Catalog # IC006P) and Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TGF-beta RI/ALK-5

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor, originally known as ALK-5 (Activin receptor-like kinase) is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

References
  1. Miyazono, K. et al. (1994) Adv. In Immunol. 55:181.
  2. Massagùe, J. (1998) Ann. Rev. Biochem. 67:753.
Long Name
Transforming Growth Factor beta Receptor I
Entrez Gene IDs
7046 (Human); 21812 (Mouse); 29591 (Rat)
Alternate Names
AAT5; activin A receptor type II-like kinase, 53kD; activin A receptor type II-like kinase, 53kDa; Activin receptor-like kinase 5; ACVRLK4; ALK-5; ALK-5ALK5; EC 2.7.11; EC 2.7.11.30; LDS1A; LDS2A; Serine/threonine-protein kinase receptor R4; SKR4; tbetaR-I; TGFB1R1; TGF-beta receptor type I; TGF-beta receptor type-1; TGF-beta RI; TGF-beta type I receptor; TGFbetaRI; TGFBR1; TGF-bRI; TGFR-1; transforming growth factor beta receptor I; transforming growth factor, beta receptor 1; transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD); Transforming growth factor-beta receptor type I

Product Datasheets

Citation for Mouse TGF-beta  RI/ALK-5 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Lin28b Regulates Fetal Regulatory T Cell Differentiation through Modulation of TGF-? Signaling
    Authors: Joseph M McCune
    J. Immunol., 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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