Mouse TGF-beta  RI/ALK-5 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5871P-100
FAB5871P-025
Detection of TGF‑ beta  RI/ALK‑5 in Mouse Splenocytes by Flow Cytometry.
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Citations (2)
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Mouse TGF-beta  RI/ALK-5 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detect mouse TGF-beta RI/ALK-5 in flow cytometry. Although this clone detects human and mouse TGF-beta RI/ALK-5 in Western blots, positive results have not been observed on human TGF-beta RI/ALK-5 in flow cytometry. In direct ELISAs and Western blots, this antibody shows no cross-reactivity with rrMIS RII, rhTGF‑ beta  RII, rhTGF‑ beta  RIIb, or rhTGF‑ beta  RIII.
Source
Monoclonal Rat IgG2A Clone # 141231
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF‑ beta  RI/ALK-5
Ala21-Glu121
Accession # BAA05023
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TGF-beta  RI/ALK-5 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of TGF‑ beta RI/ALK‑5 in Mouse Splenocytes by Flow Cytometry. NSA Mouse splenocytes were stained with (A) Rat Anti-Mouse TGF-beta RI/ALK-5 PE-conjugated Monoclonal Antibody (Catalog # FAB5871P) or Rat IgG2a isotype control antibody (Catalog # IC006P) and Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TGF-beta RI/ALK-5

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor, originally known as ALK-5 (Activin receptor-like kinase) is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

References
  1. Miyazono, K. et al. (1994) Adv. In Immunol. 55:181.
  2. Massagùe, J. (1998) Ann. Rev. Biochem. 67:753.
Long Name
Transforming Growth Factor beta Receptor I
Entrez Gene IDs
7046 (Human); 21812 (Mouse); 29591 (Rat)
Alternate Names
AAT5; activin A receptor type II-like kinase, 53kD; activin A receptor type II-like kinase, 53kDa; Activin receptor-like kinase 5; ACVRLK4; ALK-5; ALK-5ALK5; EC 2.7.11; EC 2.7.11.30; LDS1A; LDS2A; Serine/threonine-protein kinase receptor R4; SKR4; tbetaR-I; TGFB1R1; TGF-beta receptor type I; TGF-beta receptor type-1; TGF-beta RI; TGF-beta type I receptor; TGFbetaRI; TGFBR1; TGF-bRI; TGFR-1; transforming growth factor beta receptor I; transforming growth factor, beta receptor 1; transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD); Transforming growth factor-beta receptor type I

Product Datasheets

Citations for Mouse TGF-beta  RI/ALK-5 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Self-reactive B cells in the GALT are actively curtailed to prevent gut inflammation
    Authors: A Shukla, C Chen, J Jellusova, CR Leung, E Kao, N Bhat, WW Lin, JR Apgar, RC Rickert
    JCI Insight, 2019;5(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Lin28b Regulates Fetal Regulatory T Cell Differentiation through Modulation of TGF-? Signaling
    Authors: Joseph M McCune
    J. Immunol., 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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