Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Thrombospondin-4 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Thrombospondin-4
Gln27-Asn963 (predicted)
Accession # Q9Z1T2

Specificity

Detects mouse Thrombospondin-4 in direct ELISAs and Western blots. In direct ELISAs, aproximately 10% cross-reactivity with recombinant human Thrombospondin-4 is observed, and less than 1% cross-reactivity with recombinant mouse Thrombospondin-1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Thrombospondin‑4 Antibody

Detection of Mouse Thrombospondin-4 antibody by Western Blot.

Detection of Mouse Thrombospondin‑4 by Western Blot.

Western blot shows lysates of mouse muscle tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Thrombospondin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7860) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Thrombospondin-4 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Thrombospondin-4 antibody in Mouse Skeletal Muscle by Immunohistochemistry (IHC-Fr).

Thrombospondin‑4 in Mouse Skeletal Muscle.

Thrombospondin-4 was detected in perfusion fixed frozen sections of mouse skeletal muscle using Sheep Anti-Mouse Thrombospondin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7860) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Thrombospondin‑4 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse skeletal muscle

Western Blot

1 µg/mL
Sample: Mouse muscle tissue

Reviewed Applications

Read 1 review rated 4 using AF7860 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thrombospondin-4

Mouse Thrombospondin-4 (TSP-4) is a 120-130 kDa adhesive glycoprotein that belongs to the thrombospondin family of proteins.  It is 963 amino acids (aa) in length and highly modular in structure, containing one Lamnin G-like domain, four EGF-like domains and eight type 3 TSP domains.  It circulates as a disulfide-linked homopentamer, and is up-regulated in the heart in response to ischemic injury and pathology. Mature mouse TSP-4 is 93% aa identical to human TSP4.     

Alternate Names

THBS4, Thrombospondin4, TSP-4

Entrez Gene IDs

7060 (Human); 21828 (Mouse)

Gene Symbol

THBS4

UniProt

Q9Z1T2 (Mouse)

Additional Thrombospondin-4 Products

Product Documents for Mouse Thrombospondin‑4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Thrombospondin‑4 Antibody

For research use only

Citations for Mouse Thrombospondin‑4 Antibody

Customer Reviews for Mouse Thrombospondin‑4 Antibody (1)

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  • Mouse Thrombospondin-4 Antibody
    Name: marjorie maillet
    Application: Immunohistochemistry
    Sample Tested: MEFs whole cell lysate
    Species: Rat
    Verified Customer | Posted 03/31/2017

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Protocols

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FAQs

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Associated Pathways

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