Mouse Thrombospondin-4 Antibody

Catalog # Availability Size / Price Qty
AF7860
AF7860-SP
Detection of Mouse Thrombospondin‑4 by Western Blot.
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Citations (1)
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Mouse Thrombospondin-4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Thrombospondin-4 in direct ELISAs and Western blots. In direct ELISAs, aproximately 10% cross-reactivity with recombinant human Thrombospondin-4 is observed, and less than 1% cross-reactivity with recombinant mouse Thrombospondin-1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Thrombospondin-4
Gln27-Asn963 (predicted)
Accession # Q9Z1T2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse Thrombospondin‑4 by Western Blot. View Larger

Detection of Mouse Thrombospondin‑4 by Western Blot. Western blot shows lysates of mouse muscle tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Thrombospondin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7860) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Thrombospondin‑4 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Thrombospondin‑4 in Mouse Skeletal Muscle. View Larger

Thrombospondin‑4 in Mouse Skeletal Muscle. Thrombospondin‑4 was detected in perfusion fixed frozen sections of mouse skeletal muscle using Sheep Anti-Mouse Thrombospondin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7860) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombospondin-4

Mouse Thrombospondin-4 (TSP-4) is a 120-130 kDa adhesive glycoprotein that belongs to the thrombospondin family of proteins.  It is 963 amino acids (aa) in length and highly modular in structure, containing one Lamnin G-like domain, four EGF-like domains and eight type 3 TSP domains.  It circulates as a disulfide-linked homopentamer, and is up-regulated in the heart in response to ischemic injury and pathology. Mature mouse TSP-4 is 93% aa identical to human TSP4.     

Entrez Gene IDs
7060 (Human); 21828 (Mouse)
Alternate Names
THBS4; thrombospondin 4; Thrombospondin4; Thrombospondin-4; TSP4; TSP-4

Product Datasheets

Citation for Mouse Thrombospondin-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Extracellular matrix/integrin signaling promotes resistance to combined inhibition of HER2 and PI3K in HER2+ breast cancer
    Authors: AB Hanker, MV Estrada, G Bianchini, PD Moore, J Zhao, F Cheng, L Gianni, JP Koch, DR Tyson, V Sanchez, BN Rexer, M Sanders, Z Zhao, T Stricker, CL Arteaga
    Cancer Res., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Secondary Antibodies

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