Detects mouse Thrombospondin-4 in direct ELISAs and Western blots. In direct ELISAs, aproximately 10% cross-reactivity with recombinant human Thrombospondin-4 is observed, and less than 1% cross-reactivity with recombinant mouse Thrombospondin-1 is observed.
Polyclonal Sheep IgG
Chinese hamster ovary cell line CHO-derived recombinant mouse Thrombospondin-4 Thr29--Asn963 Accession # Q9Z1T2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse Thrombospondin‑4 by Western Blot. Western blot shows lysates of mouse muscle tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Thrombospondin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7860) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Thrombospondin‑4 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Thrombospondin‑4 in Mouse Skeletal Muscle. Thrombospondin‑4 was detected in perfusion fixed frozen sections of mouse skeletal muscle using Sheep Anti-Mouse Thrombospondin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7860) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse Thrombospondin-4 (TSP-4) is a 120-130 kDa adhesive glycoprotein that belongs to the thrombospondin family of proteins. It is 963 amino acids (aa) in length and highly modular in structure, containing one Lamnin G-like domain, four EGF-like domains and eight type 3 TSP domains. It circulates as a disulfide-linked homopentamer, and is up-regulated in the heart in response to ischemic injury and pathology. Mature mouse TSP-4 is 93% aa identical to human TSP4.
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