Mouse TNF RI/TNFRSF1A Antibody

Catalog # Availability Size / Price Qty
MAB430-SP
MAB430-500
MAB430-100
Mouse TNF RI/TNFRSF1A Antibody in Neutralization
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Product Details
Citations (7)
FAQs
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Reviews (1)

Mouse TNF RI/TNFRSF1A Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human
(rh) 4‑1BB, rhCD27, rhCD30, recombinant mouse (rm) CD27, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rmEDAR, rhFas, rmFas, rhEDAR, rhGITR, rmGITR, rhHVEM, rhLT beta R, rmLT beta R, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rhTNF RII or rmTNF RII is observed.
Source
Monoclonal Hamster IgG Clone # 55R170
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant mouse TNF RI/TNFRSF1A
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse TNF RI/TNFRSF1A (Catalog # 425-R1)
under non-reducing conditions only
Immunoprecipitation
Sheehan, K. et al. (1995) J. Exp. Med. 181:607.
 
Neutralization
Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 0.1 ng/mL Recombinant Mouse TNF‑ alpha and 1 µg/mL actinomycin D.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cytotoxicity Induced byTNF‑ alpha  and Neutralization by Mouse TNF RI/TNFRSF1A Antibody. View Larger

Cytotoxicity Induced by
TNF‑ alpha and Neutralization by Mouse TNF RI/TNFRSF1A Antibody.
Recombinant Mouse TNF‑ alpha (Catalog # 410-MT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF‑ alpha (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti-Mouse TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB430). The ND50 is typically 5-20 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RI/TNFRSF1A

TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a type I transmembrane protein member of the TNF receptor superfamily, designated TNFRSF1A (1, 2). Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-alpha trimer-binding cysteine-rich domains (CRD) in their extracellular domains (ECD). However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1‑3). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (4). TNF RI is present on the cell surface as a trimer of 55 kDa subunits (4, 5). TNF-alpha induces sequestering of TNF RI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (9, 10). Release of the 28 - 34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 6‑8). Full-length TNF RI may also be released in exosome-like vesicles (11). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (6, 12, 13). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Mouse TNF RI is a 454 amino acid (aa) protein that contains a 21 aa signal sequence, a 191 aa ECD with a PLAD domain (5) that mediates constitutive trimer formation, followed by the four CRD, a 23 aa transmembrane domain, and a 219 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (15). The ECD of mouse TNF RI shows 67%, 70%, 64%, 70% and 88% aa identity with canine, feline, porcine, human, and rat TNF RI, respectively; it shows 23% aa identity with the ECD of TNF RII.

References
  1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
  4. Banner, D.W et al. (1993) Cell 73: 431.
  5. Chan, F.K. et al. (2000) Science 288:2351.
  6. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
  7. Jin, L. et al. (2000) J. Immunol. 165:5153.
  8. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
  9. Legler, D.F. et al. (2003) Immunity 18:655.
  10. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
  11. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
  12. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
  13. McDermott, M.F. et al. (1999) Cell 97:133.
  14. Schneider-Brachert, W. et al. (2004) Immunity 21:415.
  15. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.
Long Name
Tumor Necrosis Factor Receptor I
Entrez Gene IDs
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

Product Datasheets

Citations for Mouse TNF RI/TNFRSF1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Cerebral ischemia-induced angiogenesis is dependent on tumor necrosis factor receptor 1-mediated upregulation of ?5?1 and ?V?3 integrins
    J Neuroinflammation, 2016;13(1):227.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation.
    Authors: Wertz I, Newton K, Seshasayee D, Kusam S, Lam C, Zhang J, Popovych N, Helgason E, Schoeffler A, Jeet S, Ramamoorthi N, Kategaya L, Newman R, Horikawa K, Dugger D, Sandoval W, Mukund S, Zindal A, Martin F, Quan C, Tom J, Fairbrother W, Townsend M, Warming S, DeVoss J, Liu J, Dueber E, Caplazi P, Lee W, Goodnow C, Balazs M, Yu K, Kolumam G, Dixit V
    Nature, 2015;528(7582):370-5.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. TLR-mediated secretion of endoplasmic reticulum aminopeptidase 1 from macrophages.
    Authors: Goto Y, Ogawa K, Nakamura T, Hattori A, Tsujimoto M
    J Immunol, 2014;192(9):4443-52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Lipopolysaccharide triggered TNF-alpha-induced hepatocyte apoptosis in a murine non-alcoholic steatohepatitis model.
    Authors: Kudo H, Takahara T, Yata Y, Kawai K, Zhang W, Sugiyama T
    J. Hepatol., 2009;51(1):168-75.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  5. Hypoxia enhances lysosomal TNF-alpha degradation in mouse peritoneal macrophages.
    Authors: Lahat N, Rahat MA, Kinarty A, Weiss-Cerem L, Pinchevski S, Bitterman H
    Am. J. Physiol., Cell Physiol., 2008;295(1):C2-12.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  6. TLR-2 is upregulated and mobilized to the hepatocyte plasma membrane in the space of Disse and to the Kupffer cells TLR-4 dependently during acute endotoxemia in mice.
    Authors: Ojaniemi M, Liljeroos M, Harju K, Sormunen R, Vuolteenaho R, Hallman M
    Immunol. Lett., 2005;102(2):158-68.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. IL-1beta augments TNF-alpha-mediated inflammatory responses from lung epithelial cells.
    Authors: Saperstein S, Chen L, Oakes D, Pryhuber G, Finkelstein J
    J Interferon Cytokine Res, 0;29(5):273-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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Mouse TNF RI/TNFRSF1A Antibody
By Anonymous on 06/28/2016
Application: ELISA Sample Tested: purified IgG Species: Human

murine cross-reactivity studies