Mouse TNF RI/TNFRSF1A Antibody

(6 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human
    (rh) 4‑1BB, rhCD27, rhCD30, recombinant mouse (rm) CD27, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rmEDAR, rhFas, rmFas, rhEDAR, rhGITR, rmGITR, rhHVEM, rhLT beta R, rmLT beta R, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rhTNF RII or rmTNF RII is observed.
  • Source
    Monoclonal Hamster IgG Clone # 55R170
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Recombinant mouse TNF RI/TNFRSF1A
    Extracellular domain
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse TNF RI/TNFRSF1A (Catalog # 425-R1)
    under non-reducing conditions only
  • Immunoprecipitation
    Sheehan, K. et al. (1995) J. Exp. Med. 181:607.
  • Neutralization
    Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 0.1 ng/mL Recombinant Mouse TNF‑ alpha and 1 µg/mL actinomycin D.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cytotoxicity Induced by
TNF‑ alpha and Neutralization by Mouse TNF RI/TNFRSF1A Antibody.
Recombinant Mouse TNF‑ alpha (Catalog # 410-MT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF‑ alpha (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti-Mouse TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB430). The ND50 is typically 5-20 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF RI/TNFRSF1A

TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a type I transmembrane protein member of the TNF receptor superfamily, designated TNFRSF1A (1, 2). Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-alpha trimer-binding cysteine-rich domains (CRD) in their extracellular domains (ECD). However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1‑3). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (4). TNF RI is present on the cell surface as a trimer of 55 kDa subunits (4, 5). TNF-alpha induces sequestering of TNF RI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (9, 10). Release of the 28 - 34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 6‑8). Full-length TNF RI may also be released in exosome-like vesicles (11). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (6, 12, 13). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Mouse TNF RI is a 454 amino acid (aa) protein that contains a 21 aa signal sequence, a 191 aa ECD with a PLAD domain (5) that mediates constitutive trimer formation, followed by the four CRD, a 23 aa transmembrane domain, and a 219 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (15). The ECD of mouse TNF RI shows 67%, 70%, 64%, 70% and 88% aa identity with canine, feline, porcine, human, and rat TNF RI, respectively; it shows 23% aa identity with the ECD of TNF RII.

  • References:
    1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
    2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
    3. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
    4. Banner, D.W et al. (1993) Cell 73: 431.
    5. Chan, F.K. et al. (2000) Science 288:2351.
    6. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
    7. Jin, L. et al. (2000) J. Immunol. 165:5153.
    8. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
    9. Legler, D.F. et al. (2003) Immunity 18:655.
    10. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
    11. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
    12. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
    13. McDermott, M.F. et al. (1999) Cell 97:133.
    14. Schneider-Brachert, W. et al. (2004) Immunity 21:415.
    15. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.
  • Long Name:
    Tumor Necrosis Factor Receptor I
  • Entrez Gene IDs:
    7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
  • Alternate Names:
    CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Cerebral ischemia-induced angiogenesis is dependent on tumor necrosis factor receptor 1-mediated upregulation of ?5?1 and ?V?3 integrins
    J Neuroinflammation, 2016;13(1):227.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation.
    Authors: Wertz I, Newton K, Seshasayee D, Kusam S, Lam C, Zhang J, Popovych N, Helgason E, Schoeffler A, Jeet S, Ramamoorthi N, Kategaya L, Newman R, Horikawa K, Dugger D, Sandoval W, Mukund S, Zindal A, Martin F, Quan C, Tom J, Fairbrother W, Townsend M, Warming S, DeVoss J, Liu J, Dueber E, Caplazi P, Lee W, Goodnow C, Balazs M, Yu K, Kolumam G, Dixit V
    Nature, 2015;528(7582):370-5.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. TLR-mediated secretion of endoplasmic reticulum aminopeptidase 1 from macrophages.
    Authors: Goto Y, Ogawa K, Nakamura T, Hattori A, Tsujimoto M
    J Immunol, 2014;192(9):4443-52.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. Lipopolysaccharide triggered TNF-alpha-induced hepatocyte apoptosis in a murine non-alcoholic steatohepatitis model.
    Authors: Kudo H, Takahara T, Yata Y, Kawai K, Zhang W, Sugiyama T
    J. Hepatol., 2009;51(1):168-75.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  5. Hypoxia enhances lysosomal TNF-alpha degradation in mouse peritoneal macrophages.
    Authors: Lahat N, Rahat MA, Kinarty A, Weiss-Cerem L, Pinchevski S, Bitterman H
    Am. J. Physiol., Cell Physiol., 2008;295(1):C2-12.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  6. TLR-2 is upregulated and mobilized to the hepatocyte plasma membrane in the space of Disse and to the Kupffer cells TLR-4 dependently during acute endotoxemia in mice.
    Authors: Ojaniemi M, Liljeroos M, Harju K, Sormunen R, Vuolteenaho R, Hallman M
    Immunol. Lett., 2005;102(2):158-68.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
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Average Rating: 4 (Based on 1 review)

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Summary

ApplicationELISA
Sample Testedpurified IgG
SpeciesHuman

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