Mouse TNF RII/TNFRSF1B Antibody

(3 citations)   
  • Species Reactivity
  • Specificity
    Detects mouse TNF RII/TNFRSF1B  in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) TNF RII, recombinant mouse (rm) TNF RI, rhTNF RI, rm4‑1BB, or rmOPG is observed. In Western blots, approximately 25% cross-reactivity with rhDR3 and rhTNF RII is observed and no cross-reactivity with rm4‑1BB, rmCD27, rmCD30, rmCD40, rmFas, rmGITR, rhHVEM, rhNGF R, rmOPG, rmRANK, rmTNF RI, rmTROY, or rmEDAR is observed.
  • Source
    Monoclonal Hamster IgG Clone # TR7554
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse TNF RII/TNFRSF1B
    Extracellular domain
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
  • Western Blot
    1 µg/mL
    Recombinant Mouse TNF RII/TNFRSF1B (Catalog # 426-R2)
  • Flow Cytometry
    1 µg/106 cells
    See below
    • Mouse TNF RII/TNFRSF1B Sandwich Immunoassay
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse TNF RII/TNFRSF1B Antibody (Catalog # MAB426)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse TNF RII/TNFRSF1B Biotinylated Antibody (Catalog # BAF426)
  • ELISA Standard
    Recombinant Mouse sTNF RII/TNFRSF1B Protein (Catalog # 426-R2)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TNF RII/TNFRSF1B in L‑929 Mouse Cell Line by Flow Cytometry. L‑929 mouse fibroblast cell line was stained with Hamster Anti-Mouse TNF RII/
TNFRSF1B Monoclonal Antibody (Catalog # MAB426, filled histogram) or isotype control antibody (open histogram), followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody (Catalog # F0120).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF RII/TNFRSF1B

Two types of soluble TNF receptors have been identified in human serum and urine which can neutralize the biological activities of TNF-alpha and TNF-beta. These binding proteins represent truncated forms of the two types of high-affinity cell surface receptors for TNF (TNFR-p60 Type B and TNFR-p80 Type A). Soluble TNF RII corresponds to TNFR-p80 Type A. In the new TNF superfamily nomenclature, TNF RII is referred to as TNFRSF1B. These apparent soluble forms of the receptors appear to arise as a result of shedding of the extracellular domains of the membrane-bound receptors. Normal concentrations as high as 4 ng/mL are found in the serum of healthy individuals, and even higher levels may be found in some pathological conditions. Although the physiological role of these proteins is not known, it has been speculated that shedding of the soluble receptors in response to TNF release could serve as a mechanism to scavenge the TNF not immediately bound and thus localize the inflammatory response. It is also possible that the pool of TNF bound to soluble receptors could represent a reservoir for the controlled release of TNF.

  • Long Name:
    Tumor Necrosis Factor Receptor II
  • Entrez Gene IDs:
    7133 (Human); 21938 (Mouse); 156767 (Rat)
  • Alternate Names:
    CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006;177(3):1967-74.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  2. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005;302(1):172-81.
    Species: Mouse
    Sample Type: Plasma
    Application: ELISA Development
  3. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2004;231(1):103-11.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

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Secondary Antibodies
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Mouse Anti-Hamster IgG Antibody

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Mouse Anti-Hamster IgG Biotinylated Antibody

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Mouse Anti-Hamster IgG PE-conjugated Antibody

Flow F0120
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Mouse Anti-Hamster IgG APC-conjugated Antibody

Flow F0121
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