Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 632307
View all TORC2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse TORC2
Lys454-Ser612
Accession # Q3U182

Specificity

Detects mouse TORC2 in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse TORC2 Antibody

Detection of Mouse TORC2 antibody by Western Blot.

Detection of Mouse TORC2 by Western Blot.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF Membrane was probed with 2 µg/mL of Mouse TORC2 Polyclonal Antibody (Catalog # MAB6458) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TORC2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Mouse TORC2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: NIH‑3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, and RAW 264.7 mouse monocyte/macrophage cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: TORC2

TORC2, also known as CREB-regulated transcription coactivator 1 (crtc2), is a 70-80 kDa protein that functions as a coactivator for CREB1 in promoting transcription through both consensus and variant cAMP response element (CRE) sites. TORC2 activity is important in regulating the expression of genes involved in cellular energy metabolism. Its O-glycosylation triggers TORC2-dependent induction of hepatic gluconeogenic enzymes. It mediates hypothalamic glucose sensing and also the sensing of endoplasmic reticulum stress. In addition, TORC2 contributes to the transcription of HTLV-1 genes and the activation of latent EBV infections. Within aa 454-612, mouse TORC2 shares 84% and 98% aa sequence identity with human and rat TORC2, respectively.

Long Name

Transducer of Regulated CREB protein 1

Alternate Names

CRTC2

Entrez Gene IDs

200186 (Human); 74343 (Mouse); 310615 (Rat)

Gene Symbol

CRTC2

UniProt

Q3U182

Additional TORC2 Products

Product Documents for Mouse TORC2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse TORC2 Antibody

For research use only

Customer Reviews for Mouse TORC2 Antibody

There are currently no reviews for this product. Be the first to review Mouse TORC2 Antibody and earn rewards!

Have you used Mouse TORC2 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies