|Detection of Mouse TORC2 by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF Membrane was probed with 2 µg/mL of Mouse TORC2 Polyclonal Antibody (Catalog # MAB6458) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TORC2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.|
TORC2, also known as CREB-regulated transcription coactivator 1 (crtc2), is a 70-80 kDa protein that functions as a coactivator for CREB1 in promoting transcription through both consensus and variant cAMP response element (CRE) sites. TORC2 activity is important in regulating the expression of genes involved in cellular energy metabolism. Its O-glycosylation triggers TORC2-dependent induction of hepatic gluconeogenic enzymes. It mediates hypothalamic glucose sensing and also the sensing of endoplasmic reticulum stress. In addition, TORC2 contributes to the transcription of HTLV-1 genes and the activation of latent EBV infections. Within aa 454-612, mouse TORC2 shares 84% and 98% aa sequence identity with human and rat TORC2, respectively.
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