Mouse Tyro3/Dtk Antibody

Catalog # Availability Size / Price Qty
MAB759
MAB759-SP
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews

Mouse Tyro3/Dtk Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Tyro3/Dtk extracellular domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Tyro3/Dtk, recombinant mouse (rm) Mer, or rmAxl is observed.
Source
Monoclonal Rat IgG1 Clone # 109646
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Tyro3/Dtk
Ala31-Ser418
Accession # P55144
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Tyro3/Dtk Fc Chimera (Catalog # 759-DT)
Flow Cytometry
2.5 µg/106 cells
Mouse embryoid bodies differentiated from D3 mouse embryonic stem cell line
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of mouse embryoid body differentiated from D3 mouse embryonic stem cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Mouse Tyro3/Dtk Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Tyro3/Dtk Biotinylated Antibody (Catalog # BAF759)

Standard: Recombinant Mouse Tyro3/Dtk Fc Chimera Protein, CF (Catalog # 759-DT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tyro3/Dtk

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

References
  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Tyrosine Protein Kinase Receptor TYRO3
Entrez Gene IDs
7301 (Human); 22174 (Mouse)
Alternate Names
Brt; BYK; Dtk; EC 2.7.10; EC 2.7.10.1; FLJ16467; Rse; Sky; Tif; TYRO3 protein tyrosine kinase; Tyro3; Tyrosine-protein kinase byk; Tyrosine-protein kinase DTK; tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY

Product Datasheets

Citations for Mouse Tyro3/Dtk Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Pan-TAM tyrosine kinase inhibitor BMS-777607 enhances anti-PD-1 mAb efficacy in a murine model of triple-negative breast cancer
    Authors: RB Birge, C Kasikara, V Davra, D Calianese, K Geng, TE Spires, M Quigley, M Wichroski, G Sriram, L Suarez-Lop, MB Yaffe, SV Kotenko, MS De Lorenzo
    Cancer Res., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Gas6 Attenuates Sepsis-Induced Tight Junction Injury and Vascular Endothelial Hyperpermeability via the Axl/NF-kappaB Signaling Pathway
    Authors: J Ni, M Lin, Y Jin, J Li, Y Guo, J Zhou, G Hong, G Zhao, Z Lu
    Front Pharmacol, 2019;10(0):662.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. GAS6 receptor status is associated with dormancy and bone metastatic tumor formation.
    Authors: Taichman, Russell, Patel, Lalit R, Bedenis, Rachel, Wang, Jingchen, Weidner, Savannah, Schumann, Taibrian, Yumoto, Kenji, Berry, Janice E, Shiozawa, Yusuke, Pienta, Kenneth
    PLoS ONE, 2013;8(4):e61873.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Toll-like receptor-mediated inhibition of Gas6 and ProS expression facilitates inflammatory cytokine production in mouse macrophages.
    Authors: Deng T, Zhang Y, Chen Q, Yan K, Han D
    Immunology, 2012;135(1):40-50.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Toll-like receptor-initiated testicular innate immune responses in mouse Leydig cells.
    Authors: Shang T, Zhang X, Wang T, Sun B, Deng T, Han D
    Endocrinology, 2011;152(7):2827-36.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. The Axl receptor tyrosine kinase is a discriminator of macrophage function in the inflamed lung.
    Authors: Fujimori T, Grabiec A, Kaur M, Bell T, Fujino N, Cook P, Svedberg F, MacDonald A, Maciewicz R, Singh D, Hussell T
    Mucosal Immunol, 0;8(5):1021-30.
    Species: Mouse
    Sample Types: BALF
    Applications: Flow Cytometry

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