Mouse VE-Cadherin Antibody

Catalog # Availability Size / Price Qty
AF1002
AF1002-SP
Detection of Mouse VE‑Cadherin by Western Blot.
3 Images
Product Details
Citations (28)
FAQs
Supplemental Products
Reviews

Mouse VE-Cadherin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse VE-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant human VE-Cadherin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VE-Cadherin
Asp46-Gln592
Accession # 2208309A
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse VE‑Cadherin by Western Blot. View Larger

Detection of Mouse VE‑Cadherin by Western Blot. Western blot shows lysates of mouse lung tissue and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1002) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VE‑Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry VE‑Cadherin  in Mouse Heart. View Larger

VE‑Cadherin in Mouse Heart.
VE‑Cadherin was detected in immersion fixed paraffin-embedded sections of mouse heart using Goat Anti-Mouse VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1002) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection  of Mouse VE‑Cadherin by Simple WesternTM. View Larger

Detection of Mouse VE‑Cadherin by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. Specific bands were detected for VE‑Cadherin at approximately 118, 146 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1002). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.        

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VE-Cadherin

The cadherin (Ca++-dependent adherence) superfamily is a large group of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least five major subfamilies based on molecule gene structure, and/or extracellular (EC) and intracellular domains (1-4). Subfamilies include classical/type I, atypical/type II, and desmosomal-related cadherins (1-3). VE-Cadherin (vascular endothelial cadherin; also cadherin-5 and CD144) is a 125 kDa atypical/type II subfamily cadherin. Its subfamily classification is based principally on its genomic structure, as its physical structure is notably divergent from other type II subfamily members (2, 3). Mouse VE-Cadherin is synthesized as a 784 amino acid (aa) type I transmembrane (TM) preproprotein that contains a 24 aa signal peptide, a 21 aa prosequence, a 554 aa extracellular region (ECR), a 21 aa TM segment, and a 164 aa cytoplasmic domain (5, 6). The ECR contains five Ca++-binding cadherin domains that are approximately 105 aa in length. Cadherin domains are comprised of two beta ‑sheets that are oriented like bread in a sandwich. Although complex, the N-terminal cadherin domain mediates trans interactions, while the internal domains contribute to cis multimerizations (7). Mouse VE-Cadherin ECR is 92%, 77%, and 73% aa identical to rat, human and porcine VE-Cadherin ECR, respectively. VE-Cadherin is involved in the maintenance of endothelial permeability. In this regard, VE-Cadherin does not initiate new blood vessel formation; it maintains it once formed. Thus, when VE‑Cadherin is downregulated, cells part and permeability increases (8). Notably, VEGF is known to promote vascular leakage, and apparently does so by inducing a beta ‑arrestin-dependent endocytosis of VE-Cadherin (9). Part of this effect may be mediated by VE‑Cadherin itself which is reported to increase the membrane half-life of VEGF R2 (10). VE-Cadherin acts homotypically at sites of zonula adherens. On each expressing cell, it is proposed that VE-Cadherin first forms a trimer, which then dimerizes with a trimeric counterpart in-trans. Alternatively, two cis-dimers could act in-trans to generate homotypic binding (11). In addition to cell adhesion, VE‑Cadherin also is reported to mediate TGF-beta receptor assembly. When clustered, VE‑Cadherin enhances T beta RII/T beta RI assembly into an active receptor complex on endothelial cells (12). VE-Cadherin is expressed on endothelial cells, trophoblast cells, endothelial progenitor cells and embryonic hematopoietic cells (5, 8, 13, 14).

References
  1. Patel, S.D. et al. (2007) Curr. Opin. Struct. Biol. 13:690.
  2. Vestweber, D. (2008) Arterioscler. Thromb. Vasc. Biol. 28:223.
  3. Vincent, P.A. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C987.
  4. Cavallaro, U. et al. (2006) Exp. Cell Res. 312:659.
  5. Breier, G. et al. (1996) Blood 87:630.
  6. Huber, P. et al. (1996) Genomics 32:21.
  7. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  8. Crosby, C.V. et al. (2005) Blood 105:2771.
  9. Gavard, J. and J.S. Gutkind (2006) Nat. Cell Biol. 8:1223.
  10. Calera, M.R. et al. (2004) Exp. Cell Res. 300:248.
  11. Hewat, E.A. et al. (2007) J. Mol. Biol. 365:744.
  12. Rudini, N. et al. (2008) EMBO J. 27:993.
  13. Kogata, N. et al. (2006) Circ. Res. 98:897.
  14. Ema, M. et al. (2006) Blood 108:4018.
Long Name
Vascular Endothelium Cadherin
Entrez Gene IDs
1003 (Human); 12562 (Mouse)
Alternate Names
7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-Cadherin

Product Datasheets

Citations for Mouse VE-Cadherin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. The bone marrow is patrolled by NK cells that are primed and expand in response to systemic viral activation
    Authors: I Milo, R Blecher-Go, Z Barnett-It, R Bar-Ziv, O Tal, I Gurevich, T Feferman, I Drexler, I Amit, P Bousso, G Shakhar
    Eur. J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. CD163+ macrophages promote angiogenesis and vascular permeability accompanied by inflammation in atherosclerosis
    Authors: L Guo, H Akahori, E Harari, SL Smith, R Polavarapu, V Karmali, F Otsuka, RL Gannon, RE Braumann, MH Dickinson, A Gupta, AL Jenkins, MJ Lipinski, J Kim, P Chhour, PS de Vries, H Jinnouchi, R Kutys, H Mori, MD Kutyna, S Torii, A Sakamoto, CU Choi, Q Cheng, ML Grove, MA Sawan, Y Zhang, Y Cao, FD Kolodgie, DP Cormode, DE Arking, E Boerwinkle, AC Morrison, J Erdmann, N Sotoodehni, R Virmani, AV Finn
    J. Clin. Invest., 2018;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Polarized actin and VE-cadherin dynamics regulate junctional remodelling and cell migration during sprouting angiogenesis
    Authors: J Cao, M Ehling, S März, J Seebach, K Tarbashevi, T Sixta, ME Pitulescu, AC Werner, B Flach, E Montanez, E Raz, RH Adams, H Schnittler
    Nat Commun, 2017;8(1):2210.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Mutual regulation of tumour vessel normalization and immunostimulatory reprogramming
    Authors: L Tian, A Goldstein, H Wang, H Ching Lo, I Sun Kim, T Welte, K Sheng, LE Dobrolecki, X Zhang, N Putluri, TL Phung, SA Mani, F Stossi, A Sreekumar, MA Mancini, WK Decker, C Zong, MT Lewis, XH Zhang
    Nature, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Embryonic Stem Cell Differentiation to Functional Arterial Endothelial Cells through Sequential Activation of ETV2 and NOTCH1 Signaling by HIF1?
    Authors: KM Tsang, JS Hyun, KT Cheng, M Vargas, D Mehta, M Ushio-Fuka, L Zou, KV Pajcini, J Rehman, AB Malik
    Stem Cell Reports, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  6. Niche-mediated depletion of the normal hematopoietic stem cell reservoir by Flt3-ITD-induced myeloproliferation
    Authors: AJ Mead, WH Neo, N Barkas, S Matsuoka, A Giustacchi, R Facchini, S Thongjuea, L Jamieson, CAG Booth, N Fordham, C Di Genua, D Atkinson, O Chowdhury, E Repapi, N Gray, S Kharazi, SA Clark, T Bouriez, P Woll, T Suda, C Nerlov, SEW Jacobsen
    J. Exp. Med., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells
    Nat Commun, 2016;7(0):12422.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  8. Targeting of Mesenchymal Stromal Cells by Cre-Recombinase Transgenes Commonly Used to Target Osteoblast Lineage Cells
    Authors: Jingzhu Zhang
    J Bone Miner Res, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC OCT-embedded
  9. Granzyme B Deficiency Protects against Angiotensin II-Induced Cardiac Fibrosis.
    Authors: Shen Y, Cheng F, Sharma M, Merkulova Y, Raithatha S, Parkinson L, Zhao H, Westendorf K, Bohunek L, Bozin T, Hsu I, Ang L, Williams S, Bleackley R, Eriksson J, Seidman M, McManus B, Granville D
    Am J Pathol, 2016;186(1):87-100.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: WB
  10. HDL activation of endothelial sphingosine-1-phosphate receptor-1 (S1P1) promotes regeneration and suppresses fibrosis in the liver
    JCI Insight, 2016;1(21):e87058.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  11. Vascular dysfunction and increased metastasis of B16F10 melanomas in Shb deficient mice as compared with their wild type counterparts.
    Authors: Zang G, Gustafsson K, Jamalpour M, Hong J, Genove G, Welsh M
    BMC Cancer, 2015;15(0):234.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases.
    Authors: Yin H, Frontini M, Arpino J, Nong Z, O'Neil C, Xu Y, Balint B, Ward A, Chakrabarti S, Ellis C, Gros R, Pickering J
    J Biol Chem, 2015;290(36):22127-42.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  13. Neuroligin 1 induces blood vessel maturation by cooperating with the alpha6 integrin.
    Authors: Samarelli A, Riccitelli E, Bizzozero L, Silveira T, Seano G, Pergolizzi M, Vitagliano G, Cascone I, Carpentier G, Bottos A, Primo L, Bussolino F, Arese M
    J Biol Chem, 2014;289(28):19466-76.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Divergent angiocrine signals from vascular niche balance liver regeneration and fibrosis.
    Authors: Ding B, Cao Z, Lis R, Nolan D, Guo P, Simons M, Penfold M, Shido K, Rabbany S, Rafii S
    Nature, 2014;505(7481):97-102.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  15. Blood vessels pattern heparan sulfate gradients between their apical and basolateral aspects.
    Authors: Stoler-Barak, Liat, Moussion, Christin, Shezen, Elias, Hatzav, Miki, Sixt, Michael, Alon, Ronen
    PLoS ONE, 2014;9(1):e85699.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Control of retinoid levels by CYP26B1 is important for lymphatic vascular development in the mouse embryo.
    Authors: Bowles J, Secker G, Nguyen C, Kazenwadel J, Truong V, Frampton E, Curtis C, Skoczylas R, Davidson T, Miura N, Hong Y, Koopman P, Harvey N, Francois M
    Dev Biol, 2014;386(1):25-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  17. Transcriptional targeting of primary and metastatic tumor neovasculature by an adenoviral type 5 roundabout4 vector in mice.
    Authors: Lu, Zhi Hong, Kaliberov, Sergey, Sohn, Rebecca, Kaliberova, Lyudmila, Curiel, David T, Arbeit, Jeffrey
    PLoS ONE, 2013;8(12):e83933.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  18. Flk1+ and VE-cadherin+ endothelial cells derived from iPSCs recapitulates vascular development during differentiation and display similar angiogenic potential as ESC-derived cells.
    Authors: Kohler, Erin E, Wary, Kishore, Li, Fei, Chatterjee, Ishita, Urao, Norifumi, Toth, Peter T, Ushio-Fukai, Masuko, Rehman, Jalees, Park, Changwon, Malik, Asrar B
    PLoS ONE, 2013;8(12):e85549.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  19. VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd.
    Authors: Sun Z, Li X, Massena S, Kutschera S, Padhan N, Gualandi L, Sundvold-Gjerstad V, Gustafsson K, Choy WW, Zang G, Quach M, Jansson L, Phillipson M, Abid MR, Spurkland A, Claesson-Welsh L
    J. Exp. Med., 2012;209(7):1363-77.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  20. Manganese superoxide dismutase expression in endothelial progenitor cells accelerates wound healing in diabetic mice.
    Authors: Marrotte EJ, Chen DD, Hakim JS
    J. Clin. Invest., 2010;120(12):4207-19.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  21. Endothelial cells are essential for the self-renewal and repopulation of Notch-dependent hematopoietic stem cells.
    Authors: Butler JM, Nolan DJ, Vertes EL, Varnum-Finney B, Kobayashi H, Hooper AT, Seandel M, Shido K, White IA, Kobayashi M, Witte L, May C, Shawber C, Kimura Y, Kitajewski J, Rosenwaks Z, Bernstein ID, Rafii S
    Cell Stem Cell, 2010;6(3):251-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  22. Heterozygous deficiency of PHD2 restores tumor oxygenation and inhibits metastasis via endothelial normalization.
    Authors: Mazzone M, Dettori D, Leite de Oliveira R, Loges S, Schmidt T, Jonckx B, Tian YM, Lanahan AA, Pollard P, Ruiz de Almodovar C, De Smet F, Vinckier S, Aragones J, Debackere K, Luttun A, Wyns S, Jordan B, Pisacane A, Gallez B, Lampugnani MG, Dejana E, Simons M, Ratcliffe P, Maxwell P, Carmeliet P
    Cell, 2009;136(5):839-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  23. Dysfunctional microvasculature as a consequence of shb gene inactivation causes impaired tumor growth.
    Authors: Funa NS, Kriz V, Zang G, Calounova G, Akerblom B, Mares J, Larsson E, Sun Y, Betsholtz C, Welsh M
    Cancer Res., 2009;69(5):2141-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Enhancing the reliability and throughput of neurosphere culture on hydrogel microwell arrays.
    Authors: Cordey M, Limacher M, Kobel S, Taylor V, Lutolf MP
    Stem Cells, 2008;26(10):2586-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  25. Genetic delivery of the murine equivalent of bevacizumab (avastin), an anti-vascular endothelial growth factor monoclonal antibody, to suppress growth of human tumors in immunodeficient mice.
    Authors: Watanabe M, Boyer JL, Hackett NR, Qiu J, Crystal RG
    Hum. Gene Ther., 2008;19(3):300-10.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  26. Lentiviral rescue of vascular endothelial growth factor receptor-2 expression in flk1-/- embryonic stem cells shows early priming of endothelial precursors.
    Authors: Li X, Edholm D, Lanner F, Breier G, Farnebo F, Dimberg A, Claesson-Welsh L
    Stem Cells, 2007;25(12):2987-95.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  27. Effect of gestational nutrition on vascular integrity in the murine placenta.
    Authors: Rutland CS, Latunde-Dada AO, Thorpe A, Plant R, Langley-Evans S, Leach L
    Placenta, 2006;28(7):734-42.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  28. Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes.
    Authors: Liu Q, Yang R, Huang X, Zhang H, He L, Zhang L, Tian X, Nie Y, Hu S, Yan Y, Zhang L, Qiao Z, Wang Q, Lui K, Zhou B
    Cell Res, 0;26(1):119-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse VE-Cadherin Antibody

There are currently no reviews for this product. Be the first to review Mouse VE-Cadherin Antibody and earn rewards!

Have you used Mouse VE-Cadherin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review