Mouse VE-Cadherin Antibody AF1002: R&D Systems

Mouse VE-Cadherin Antibody

(23 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse VE-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant human VE-Cadherin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse VE-Cadherin
    Asp46-Gln592
    Accession # 2208309A
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse VE‑Cadherin by Western Blot. Western blot shows lysates of mouse lung tissue and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1002) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VE‑Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VE-Cadherin

The cadherin (Ca++-dependent adherence) superfamily is a large group of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least five major subfamilies based on molecule gene structure, and/or extracellular (EC) and intracellular domains (1-4). Subfamilies include classical/type I, atypical/type II, and desmosomal-related cadherins (1-3). VE-Cadherin (vascular endothelial cadherin; also cadherin-5 and CD144) is a 125 kDa atypical/type II subfamily cadherin. Its subfamily classification is based principally on its genomic structure, as its physical structure is notably divergent from other type II subfamily members (2, 3). Mouse VE-Cadherin is synthesized as a 784 amino acid (aa) type I transmembrane (TM) preproprotein that contains a 24 aa signal peptide, a 21 aa prosequence, a 554 aa extracellular region (ECR), a 21 aa TM segment, and a 164 aa cytoplasmic domain (5, 6). The ECR contains five Ca++-binding cadherin domains that are approximately 105 aa in length. Cadherin domains are comprised of two beta ‑sheets that are oriented like bread in a sandwich. Although complex, the N-terminal cadherin domain mediates trans interactions, while the internal domains contribute to cis multimerizations (7). Mouse VE-Cadherin ECR is 92%, 77%, and 73% aa identical to rat, human and porcine VE-Cadherin ECR, respectively. VE-Cadherin is involved in the maintenance of endothelial permeability. In this regard, VE-Cadherin does not initiate new blood vessel formation; it maintains it once formed. Thus, when VE‑Cadherin is downregulated, cells part and permeability increases (8). Notably, VEGF is known to promote vascular leakage, and apparently does so by inducing a beta ‑arrestin-dependent endocytosis of VE-Cadherin (9). Part of this effect may be mediated by VE‑Cadherin itself which is reported to increase the membrane half-life of VEGF R2 (10). VE-Cadherin acts homotypically at sites of zonula adherens. On each expressing cell, it is proposed that VE-Cadherin first forms a trimer, which then dimerizes with a trimeric counterpart in-trans. Alternatively, two cis-dimers could act in-trans to generate homotypic binding (11). In addition to cell adhesion, VE‑Cadherin also is reported to mediate TGF-beta receptor assembly. When clustered, VE‑Cadherin enhances T beta RII/T beta RI assembly into an active receptor complex on endothelial cells (12). VE-Cadherin is expressed on endothelial cells, trophoblast cells, endothelial progenitor cells and embryonic hematopoietic cells (5, 8, 13, 14).

  • References:
    1. Patel, S.D. et al. (2007) Curr. Opin. Struct. Biol. 13:690.
    2. Vestweber, D. (2008) Arterioscler. Thromb. Vasc. Biol. 28:223.
    3. Vincent, P.A. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C987.
    4. Cavallaro, U. et al. (2006) Exp. Cell Res. 312:659.
    5. Breier, G. et al. (1996) Blood 87:630.
    6. Huber, P. et al. (1996) Genomics 32:21.
    7. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
    8. Crosby, C.V. et al. (2005) Blood 105:2771.
    9. Gavard, J. and J.S. Gutkind (2006) Nat. Cell Biol. 8:1223.
    10. Calera, M.R. et al. (2004) Exp. Cell Res. 300:248.
    11. Hewat, E.A. et al. (2007) J. Mol. Biol. 365:744.
    12. Rudini, N. et al. (2008) EMBO J. 27:993.
    13. Kogata, N. et al. (2006) Circ. Res. 98:897.
    14. Ema, M. et al. (2006) Blood 108:4018.
  • Long Name:
    Vascular Endothelium Cadherin
  • Entrez Gene IDs:
    1003 (Human); 12562 (Mouse)
  • Alternate Names:
    7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-Cadherin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Embryonic Stem Cell Differentiation to Functional Arterial Endothelial Cells through Sequential Activation of ETV2 and NOTCH1 Signaling by HIF1?
    Authors: KM Tsang, JS Hyun, KT Cheng, M Vargas, D Mehta, M Ushio-Fuka, L Zou, KV Pajcini, J Rehman, AB Malik
    Stem Cell Reports, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Mutual regulation of tumour vessel normalization and immunostimulatory reprogramming
    Authors: L Tian, A Goldstein, H Wang, H Ching Lo, I Sun Kim, T Welte, K Sheng, LE Dobrolecki, X Zhang, N Putluri, TL Phung, SA Mani, F Stossi, A Sreekumar, MA Mancini, WK Decker, C Zong, MT Lewis, XH Zhang
    Nature, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Targeting of Mesenchymal Stromal Cells by Cre-Recombinase Transgenes Commonly Used to Target Osteoblast Lineage Cells
    Authors: Jingzhu Zhang
    J Bone Miner Res, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  4. Granzyme B Deficiency Protects against Angiotensin II-Induced Cardiac Fibrosis.
    Authors: Shen Y, Cheng F, Sharma M, Merkulova Y, Raithatha S, Parkinson L, Zhao H, Westendorf K, Bohunek L, Bozin T, Hsu I, Ang L, Williams S, Bleackley R, Eriksson J, Seidman M, McManus B, Granville D
    Am J Pathol, 2016;186(1):87-100.
    Species: Mouse
    Sample Type: Recombinant Protein
    Application: WB
  5. Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells
    Nat Commun, 2016;7(0):12422.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  6. HDL activation of endothelial sphingosine-1-phosphate receptor-1 (S1P1) promotes regeneration and suppresses fibrosis in the liver
    JCI Insight, 2016;1(21):e87058.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases.
    Authors: Yin H, Frontini M, Arpino J, Nong Z, O'Neil C, Xu Y, Balint B, Ward A, Chakrabarti S, Ellis C, Gros R, Pickering J
    J Biol Chem, 2015;290(36):22127-42.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Vascular dysfunction and increased metastasis of B16F10 melanomas in Shb deficient mice as compared with their wild type counterparts.
    Authors: Zang G, Gustafsson K, Jamalpour M, Hong J, Genove G, Welsh M
    BMC Cancer, 2015;15(0):234.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  9. Divergent angiocrine signals from vascular niche balance liver regeneration and fibrosis.
    Authors: Ding B, Cao Z, Lis R, Nolan D, Guo P, Simons M, Penfold M, Shido K, Rabbany S, Rafii S
    Nature, 2014;505(7481):97-102.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IF
  10. Control of retinoid levels by CYP26B1 is important for lymphatic vascular development in the mouse embryo.
    Authors: Bowles J, Secker G, Nguyen C, Kazenwadel J, Truong V, Frampton E, Curtis C, Skoczylas R, Davidson T, Miura N, Hong Y, Koopman P, Harvey N, Francois M
    Dev Biol, 2014;386(1):25-33.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  11. Blood vessels pattern heparan sulfate gradients between their apical and basolateral aspects.
    Authors: Stoler-Barak, Liat, Moussion, Christin, Shezen, Elias, Hatzav, Miki, Sixt, Michael, Alon, Ronen
    PLoS ONE, 2014;9(1):e85699.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  12. Neuroligin 1 induces blood vessel maturation by cooperating with the alpha6 integrin.
    Authors: Samarelli A, Riccitelli E, Bizzozero L, Silveira T, Seano G, Pergolizzi M, Vitagliano G, Cascone I, Carpentier G, Bottos A, Primo L, Bussolino F, Arese M
    J Biol Chem, 2014;289(28):19466-76.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  13. Transcriptional targeting of primary and metastatic tumor neovasculature by an adenoviral type 5 roundabout4 vector in mice.
    Authors: Lu, Zhi Hong, Kaliberov, Sergey, Sohn, Rebecca, Kaliberova, Lyudmila, Curiel, David T, Arbeit, Jeffrey
    PLoS ONE, 2013;8(12):e83933.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  14. Flk1+ and VE-cadherin+ endothelial cells derived from iPSCs recapitulates vascular development during differentiation and display similar angiogenic potential as ESC-derived cells.
    Authors: Kohler, Erin E, Wary, Kishore, Li, Fei, Chatterjee, Ishita, Urao, Norifumi, Toth, Peter T, Ushio-Fukai, Masuko, Rehman, Jalees, Park, Changwon, Malik, Asrar B
    PLoS ONE, 2013;8(12):e85549.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  15. VEGFR2 induces c-Src signaling and vascular permeability in vivo via the adaptor protein TSAd.
    Authors: Sun Z, Li X, Massena S, Kutschera S, Padhan N, Gualandi L, Sundvold-Gjerstad V, Gustafsson K, Choy WW, Zang G, Quach M, Jansson L, Phillipson M, Abid MR, Spurkland A, Claesson-Welsh L
    J. Exp. Med., 2012;209(7):1363-77.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  16. Manganese superoxide dismutase expression in endothelial progenitor cells accelerates wound healing in diabetic mice.
    Authors: Marrotte EJ, Chen DD, Hakim JS
    J. Clin. Invest., 2010;120(12):4207-19.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  17. Endothelial cells are essential for the self-renewal and repopulation of Notch-dependent hematopoietic stem cells.
    Authors: Butler JM, Nolan DJ, Vertes EL, Varnum-Finney B, Kobayashi H, Hooper AT, Seandel M, Shido K, White IA, Kobayashi M, Witte L, May C, Shawber C, Kimura Y, Kitajewski J, Rosenwaks Z, Bernstein ID, Rafii S
    Cell Stem Cell, 2010;6(3):251-64.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  18. Dysfunctional microvasculature as a consequence of shb gene inactivation causes impaired tumor growth.
    Authors: Funa NS, Kriz V, Zang G, Calounova G, Akerblom B, Mares J, Larsson E, Sun Y, Betsholtz C, Welsh M
    Cancer Res., 2009;69(5):2141-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  19. Heterozygous deficiency of PHD2 restores tumor oxygenation and inhibits metastasis via endothelial normalization.
    Authors: Mazzone M, Dettori D, Leite de Oliveira R, Loges S, Schmidt T, Jonckx B, Tian YM, Lanahan AA, Pollard P, Ruiz de Almodovar C, De Smet F, Vinckier S, Aragones J, Debackere K, Luttun A, Wyns S, Jordan B, Pisacane A, Gallez B, Lampugnani MG, Dejana E, Simons M, Ratcliffe P, Maxwell P, Carmeliet P
    Cell, 2009;136(5):839-51.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  20. Genetic delivery of the murine equivalent of bevacizumab (avastin), an anti-vascular endothelial growth factor monoclonal antibody, to suppress growth of human tumors in immunodeficient mice.
    Authors: Watanabe M, Boyer JL, Hackett NR, Qiu J, Crystal RG
    Hum. Gene Ther., 2008;19(3):300-10.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  21. Enhancing the reliability and throughput of neurosphere culture on hydrogel microwell arrays.
    Authors: Cordey M, Limacher M, Kobel S, Taylor V, Lutolf MP
    Stem Cells, 2008;26(10):2586-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  22. Lentiviral rescue of vascular endothelial growth factor receptor-2 expression in flk1-/- embryonic stem cells shows early priming of endothelial precursors.
    Authors: Li X, Edholm D, Lanner F, Breier G, Farnebo F, Dimberg A, Claesson-Welsh L
    Stem Cells, 2007;25(12):2987-95.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  23. Effect of gestational nutrition on vascular integrity in the murine placenta.
    Authors: Rutland CS, Latunde-Dada AO, Thorpe A, Plant R, Langley-Evans S, Leach L
    Placenta, 2006;28(7):734-42.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
Expand to show all 23 Citations
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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