Mouse VSIG2 Antibody

Catalog # Availability Size / Price Qty
AF4766
AF4766-SP
Product Details
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Mouse VSIG2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse VSIG2 in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human (rh) VSIG2 is observed and less than 10% cross-reactivity with rhVSIG3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VSIG2
Val25-Ala244 (Glu240Asp)
Accession # Q9Z109
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse VSIG2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG2

VSIG2 (V-set and Ig domain-containing protein 2; also CTM and CT-like protein) is presumably a 50‑60 kDa member of the CTX family of Ig-Superfamily proteins. It shows expression in stomach and prostate by Northern blot, and likely participates in cell adhesion. Mouse VSIG2 precursor is 328 amino acids (aa) in length. It is a type I transmembrane (glyco)protein that contains a 220 aa extracellular domain (ECD) (aa 25‑244) and a 63 aa cytoplasmic region. The ECD contains one V-type (aa 25‑138) and one C2-type Ig-like domain (aa 145‑234). Over aa 25‑244, mouse VSIG2 is 94%, 83% and 85% aa identical to rat, canine and human VSIG2, respectively. Two potential splice variants exist, one that shows a deletion of aa 305‑328 and a second that shows a deletion of aa 144‑236.

Long Name
V-Set and Immunoglobulin Domain Containing 2
Entrez Gene IDs
23584 (Human); 57276 (Mouse); 300520 (Rat)
Alternate Names
2210413P10Rik; Cortical thymocyte-like protein; CTH; CTHCTXLcortical thymocyte receptor (X. laevis CTX) like; CT-like protein; CTXL; V-set and immunoglobulin domain containing 2; V-set and immunoglobulin domain-containing protein 2; VSIG2

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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