Mouse Wnt-5a Biotinylated Antibody

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Product Details
Citations (4)
Supplemental Products

Mouse Wnt-5a Biotinylated Antibody Summary

Species Reactivity
Detects mouse Wnt-5a in Western blots. In this format, approximately 2% cross-reactivity with recombinant mouse Wnt‑4 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant mouse Wnt‑5a peptide
Accession # P22725
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Wnt‑5a (Catalog # 645-WN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-5a

Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23 - 24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development for a variety of organisms. Wnts bind to receptors of the Frizzled family, sometimes in conjunction with other membrane-associated proteins such as LRPs or proteoglycans. Downstream effects of Wnt signaling occur through different intracellular components, depending on which pathway is activated. Three pathways have been characterized: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca2+ pathway, and the planar cell polarity (1 - 2).

Wnt-5a is part of the subgroup of Wnts that are not axis-inducing in Xenopus embryos and do not transform C57MG mammary epithelial cells. This subgroup is also implicated in the Wnt/Ca2+ pathway, playing roles in cell movements and cell adhesion (3). This non-canonical Wnt pathway can inhibit canonical Wnt/ beta -catenin signaling. In Wnt-5a deficient mouse embryos, beta -catenin accumulates in the limb bud suggesting that Wnt-5a normally promotes degradation of beta -catenin (4). Likewise, in Xenopus embryos Wnt-5a antagonizes the ability of the canonical Wnt subgroup to induce a secondary axis (5). Wnt-5a is implicated in various types of cancer and has complex roles. It acts as a tumor suppressor for mammary, B-cell, colon, and uroepithelial cancer cells but is up-regulated in melanomas, where expression levels correlate with severity of metastasis (3). Furthermore, aberrant Wnt-5a signaling results in other diseases such as rheumatoid arthritis (6). Like other developmental growth factors Wnt-5a has diverse roles in development. They are too numerous to enunciate here, as functions span from early anterior-posterior development and gastrulation movements to maintaining hematopoietic stem cell population, lung morphogenesis, and limb outgrowth. Mouse and human Wnt-5a share 97% amino acid identity.

  1. Miller, J.R. (2002) Genome Biol. 3:3001.
  2. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
  3. Veeman, M.T. et al. (2003) Developmental Cell 5:367.
  4. Topol, L. et al. (2003) J. Cell Biol 162:899.
  5. Torres, M. et al. (1996) J. Cell Biol. 133:1123.
  6. Sen, M. et al. (2001) Arthritis & Rheumatism 44:772.
Long Name
Wingless-type MMTV Integration Site Family, Member 5a
Entrez Gene IDs
7474 (Human); 22418 (Mouse)
Alternate Names
hWNT5A; protein Wnt-5a; wingless-type MMTV integration site family, member 5A; WNT-5A protein; Wnt5a; Wnt-5a

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Citations for Mouse Wnt-5a Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. ATG5 mediates a positive feedback loop between Wnt signaling and autophagy in melanoma
    Authors: A Ndoye, A Budina-Kol, CH Kugel, MR Webster, A Kaur, R Behera, VW Rebecca, L Li, PA Brafford, Q Liu, YN Vashisht G, MA Davies, GB Mills, X Xu, H Wu, M Herlyn, MC Nicastri, JD Winkler, MS Soengas, RK Amaravadi, ME Murphy, AT Weeraratna
    Cancer Res., 2017;0(0):.
  2. Inhibition of age-related therapy resistance in melanoma by rosiglitazone-mediated induction of Klotho
    Authors: R Behera, A Kaur, MR Webster, S Kim, A Ndoye, CH Kugel, GM Alicea, JX Wang, K Ghosh, PF Cheng, S Lisanti, K Marchbank, V Dang, MP Levesque, R Dummer, X Xu, M Heryn, AE Aplin, A Roesch, MC Caino, DC Altieri, AT Weeraratna
    Clin. Cancer Res, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. The orphan tyrosine kinase receptor, ROR2, mediates Wnt5A signaling in metastatic melanoma.
    Authors: O'Connell MP, Fiori JL, Xu M
    Oncogene, 2010;29(1):34-44.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Wnt5A activates the calpain-mediated cleavage of filamin A.
    Authors: O'Connell MP, Fiori JL, Baugher KM, Indig FE, French AD, Camilli TC, Frank BP, Earley R, Hoek KS, Hasskamp JH, Elias EG, Taub DD, Bernier M, Weeraratna AT
    J. Invest. Dermatol., 2009;129(7):1782-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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