Mouse/Rat Wnt-5a Antibody

  (25 citations)
(1 Review)
    
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  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse and rat Wnt-5a in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Wnt‑5b is observed and less than 2% cross-reactivity with rmWnt-1, rmWnt-3a, rmWnt-4, rmWnt-11, and rmWnt-16 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse Wnt‑5a peptide
    Gln254-Cys334
    Accession # P22725
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Wnt‑5a in Mouse Embryonic Rib. Wnt‑5a was detected in immersion fixed paraffin-embedded sections of mouse embryonic rib using 15 µg/mL Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Mouse Wnt‑5a by Western Blot. Western blot shows lysates of mouse brain and lactating mammary tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Wnt‑5a at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunohistochemistry
Wnt‑5a in Mouse Embryo. Wnt‑5a was detected in immersion fixed frozen sections of mouse embryo using Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-5a

Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23-24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development for a variety of organisms. Wnts bind to receptors of the Frizzled family, sometimes in conjunction with other membrane-associated proteins such as LRPs or proteoglycans. Downstream effects of Wnt signaling occur through different intracellular components, depending on which pathway is activated. Three pathways have been characterized: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca2+ pathway, and the planar cell polarity (1, 2).

Wnt-5a is part of the subgroup of Wnts that are not axis-inducing in Xenopus embryos and do not transform C57MG mammary epithelial cells. This subgroup is also implicated in the Wnt/Ca2+ pathway, playing roles in cell movements and cell adhesion (3). This non-canonical Wnt pathway can inhibit canonical Wnt/ beta -catenin signaling. In Wnt-5a deficient mouse embryos, beta -catenin accumulates in the limb bud suggesting that Wnt-5a normally promotes degradation of beta -catenin (4). Likewise, in Xenopus embryos Wnt-5a antagonizes the ability of the canonical Wnt subgroup to induce a secondary axis (5). Wnt-5a is implicated in various types of cancer and has complex roles. It acts as a tumor suppressor for mammary, B-cell, colon, and uroepithelial cancer cells but is up-regulated in melanomas, where expression levels correlate with severity of metastasis (3). Furthermore, aberrant Wnt-5a signaling results in other diseases such as rheumatoid arthritis (6). Like other developmental growth factors Wnt-5a has diverse roles in development. They are too numerous to enunciate here, as functions span from early anterior-posterior development and gastrulation movements to maintaining hematopoietic stem cell population, lung morphogenesis, and limb outgrowth. Mouse and human Wnt-5a share 97% amino acid identity.

  • References:
    1. Miller, J.R. (2002) Genome Biol. 3:3001.
    2. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
    3. Veeman, M.T. et al. (2003) Developmental Cell 5:367.
    4. Topol, L. et al. (2003) J. Cell Biol 162:899.
    5. Torres, M. et al. (1996) J. Cell Biol. 133:1123.
    6. Sen, M. et al. (2001) Arthritis & Rheumatism 44:772.
  • Long Name:
    Wingless-type MMTV Integration Site Family, Member 5a
  • Entrez Gene IDs:
    7474 (Human); 22418 (Mouse)
  • Alternate Names:
    hWNT5A; protein Wnt-5a; wingless-type MMTV integration site family, member 5A; WNT-5A protein; Wnt5a; Wnt-5a
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex.
    J. Cell. Sci., 2102;125(0):2017-29.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Reduced Cdc42 Activity Compromises Hematopoiesis-Supportive Function Of Fanconi Anemia Mesenchymal Stromal Cells
    Authors: J Xu, X Li, A Cole, Z Sherman, W Du
    Stem Cells, 2018;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice
    Authors: CM Chen, LL Orefice, SL Chiu, TA LeGates, S Hattar, RL Huganir, H Zhao, B Xu, R Kuruvilla
    Proc. Natl. Acad. Sci. U.S.A, 2017;114(4):E619-E628.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development
    Sci Rep, 2016;6(0):32094.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. IL-4 Causes Hyperpermeability of Vascular Endothelial Cells through Wnt5A Signaling
    PLoS ONE, 2016;11(5):e0156002.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - Not specified
  6. Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation
    J Biol Chem, 2016;0(0):.
    Species: N/A
    Sample Type: Recombinant Protein
    Application: Bioassay
  7. Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD
    Authors: Hoeke A Baarsma
    J. Exp. Med, 2016;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  8. TGF-beta-activated kinase 1 (TAK1) signaling regulates TGF-beta-induced WNT-5A expression in airway smooth muscle cells via Sp1 and beta-catenin.
    Authors: Kumawat K, Menzen M, Slegtenhorst R, Halayko A, Schmidt M, Gosens R
    PLoS ONE, 2014;9(4):e94801.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  9. WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells.
    Authors: Ekstrom E, Bergenfelz C, von Bulow V, Serifler F, Carlemalm E, Jonsson G, Andersson T, Leandersson K
    Mol Cancer, 2014;13(0):88.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  10. Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism.
    Authors: Dawes L, Sugiyama Y, Lovicu F, Harris C, Shelley E, McAvoy J
    Dev Biol, 2014;385(2):291-303.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  11. A canonical to non-canonical Wnt signalling switch in haematopoietic stem-cell ageing.
    Authors: Florian M, Nattamai K, Dorr K, Marka G, Uberle B, Vas V, Eckl C, Andra I, Schiemann M, Oostendorp R, Scharffetter-Kochanek K, Kestler H, Zheng Y, Geiger H
    Nature, 2013;503(7476):392-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IF
  12. NMDA receptor activation stimulates transcription-independent rapid wnt5a protein synthesis via the MAPK signaling pathway.
    Mol Brain, 2012;5(0):1.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  13. WNT5A Signaling Contributes to Abeta-Induced Neuroinflammation and Neurotoxicity.
    Authors: Li B, Zhong L, Yang X, Andersson T, Huang M, Tang SJ
    PLoS ONE, 2011;6(8):e22920.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  14. Wnt5a suppresses epithelial ovarian cancer by promoting cellular senescence.
    Authors: Bitler BG, Nicodemus JP, Li H, Cai Q, Wu H, Hua X, Li T, Birrer MJ, Godwin AK, Cairns P, Zhang R
    Cancer Res., 2011;71(19):6184-94.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  15. Evidence for altered Wnt signaling in psoriatic skin.
    Authors: Gudjonsson JE, Johnston A, Stoll SW
    J. Invest. Dermatol., 2010;130(7):1849-59.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. Wnt5a mediates nerve growth factor-dependent axonal branching and growth in developing sympathetic neurons.
    Authors: Bodmer D, Levine-Wilkinson S, Richmond A, Hirsh S, Kuruvilla R
    J. Neurosci., 2009;29(23):7569-81.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  17. Wnt5a is expressed in murine and human atherosclerotic lesions.
    Authors: Christman MA, Goetz DJ, Dickerson E, McCall KD, Lewis CJ, Benencia F, Silver MJ, Kohn LD, Malgor R
    Am. J. Physiol. Heart Circ. Physiol., 2008;294(6):H2864-70.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  18. Wnt5A/CaMKII signaling contributes to the inflammatory response of macrophages and is a target for the antiinflammatory action of activated protein C and interleukin-10.
    Authors: Pereira C, Schaer DJ, Bachli EB, Kurrer MO, Schoedon G
    Arterioscler. Thromb. Vasc. Biol., 2008;28(3):504-10.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  19. Dickkopf-1 is a master regulator of joint remodeling.
    Authors: Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G
    Nat. Med., 2007;13(2):156-63.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  20. The Wingless homolog WNT5A and its receptor Frizzled-5 regulate inflammatory responses of human mononuclear cells induced by microbial stimulation.
    Authors: Blumenthal A, Ehlers S, Lauber J, Buer J, Lange C, Goldmann T, Heine H, Brandt E, Reiling N
    Blood, 2006;108(3):965-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  21. Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2.
    Authors: Masckauchan TN, Agalliu D, Vorontchikhina M, Ahn A, Parmalee NL, Li CM, Khoo A, Tycko B, Brown AM, Kitajewski J
    Mol. Biol. Cell, 2006;17(12):5163-72.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  22. Ventral midbrain glia express region-specific transcription factors and regulate dopaminergic neurogenesis through Wnt-5a secretion.
    Authors: Castelo-Branco G, Sousa KM, Bryja V, Pinto L, Wagner J, Arenas E
    Mol. Cell. Neurosci., 2005;31(2):251-62.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  23. Wnt-5A augments repopulating capacity and primitive hematopoietic development of human blood stem cells in vivo.
    Authors: Murdoch B, Chadwick K, Martin M, Shojaei F, Shah KV, Gallacher L, Moon RT, Bhatia M
    Proc. Natl. Acad. Sci. U.S.A., 2003;100(6):3422-7.
    Species: Xenopus
    Sample Type: Cell Culture Supernates
    Application: Immunodepletion
  24. Wnt signaling induces the myogenic specification of resident CD45+ adult stem cells during muscle regeneration.
    Authors: Polesskaya A, Seale P, Rudnicki MA
    Cell, 2003;113(7):841-52.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  25. Signaling of rat Frizzled-2 through phosphodiesterase and cyclic GMP.
    Authors: Ahumada A, Slusarski DC, Liu X, Moon RT, Malbon CC, Wang HY
    Science, 2002;298(5600):2006-10.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: Immunodepletion
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Average Rating: 3 (Based on 1 review)

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Western Blot Mouse/Rat Wnt-5a Antibody AF645 Array AF645
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 WB Mouse Anonymous 02/19/2018
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Western Blot Mouse/Rat Wnt-5a Antibody AF645
Western Blot: Mouse/Rat Wnt-5a Antibody [AF645].

Summary

Application Western Blot
Sample Tested B16-F1 mouse melanoma cell line
Species Mouse

Other Experimental Details

Other Experimental Details Non-specific bands at 64kDa, actual band at 49KDa. Recommend to use 7.5% gel for better separation.

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