Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 254126
View all Wnt-8a Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Wnt-8a
Ala20-Trp354 (predicted)
Accession # Q64527

Specificity

Detects mouse Wnt-8a in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse Wnt-1, -3a, -4, -5a, or -10b is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Wnt-8a Antibody (254126)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Wnt‑8a

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-8a

Wnt-8a is a secreted glycoprotein that binds to receptors of the Frizzled family. It is expressed during early optic lens development and cardiac myogenesis.

Long Name

Wingless-type MMTV Integration Site Family, Member 8a

Alternate Names

Wnt8a

Entrez Gene IDs

7478 (Human); 20890 (Mouse)

Gene Symbol

WNT8A

UniProt

Q64527

Additional Wnt-8a Products

Product Documents for Mouse Wnt-8a Antibody (254126)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Wnt-8a Antibody (254126)

For research use only

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FAQs

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