Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 341502
View all Wnt-8b Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Wnt‑8b
Trp22-Glu52, Leu195-Arg269
Accession # Q9WUD6

Specificity

Detects mouse Wnt-8b in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Wnt-2, -7a, -7b,-9a, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, or -9b is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Wnt‑8b Antibody

Detection of Mouse Wnt‑8b antibody by Western Blot.

Detection of Mouse Wnt‑8b by Western Blot.

Western blot shows lysates of KATO-III human gastric carcinoma cell line. PVDF membrane was probed with 5 µg/mL Rat Anti-Mouse Wnt-8b Monoclonal Antibody (Catalog # MAB33671) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant mouse Wnt-8b (20 ng) was included. A specific band for native Wnt-8b was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

Applications for Mouse Wnt‑8b Antibody

Application
Recommended Usage

Western Blot

5 µg/mL
Sample: KATO-III human gastric carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-8b

Mouse Wnt-8b is a 40-50 kDa secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329 aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98%, and 97% identical to mature rat, canine, and human Wnt-8b, respectively.

Long Name

Wingless-type MMTV Integration Site Family, Member 8b

Alternate Names

Wnt8b

Entrez Gene IDs

7479 (Human); 22423 (Mouse); 293990 (Rat)

Gene Symbol

WNT8B

UniProt

Q9WUD6

Additional Wnt-8b Products

Product Documents for Mouse Wnt‑8b Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Wnt‑8b Antibody

For research use only

Related Research Areas

Wnt Ligands (Wnts)

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