Mouse Wnt-8b Antibody Summary
-9a, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, or -9b is observed.
Accession # Q9WUD6
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse Wnt‑8b by Western Blot. Western blot shows lysates of KATO-III human gastric carcinoma cell line. PVDF membrane was probed with 5 µg/mL Rat Anti-Mouse Wnt‑8b Monoclonal Antibody (Catalog # MAB33671) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant mouse Wnt-8b (20 ng) was included. A specific band for native Wnt-8b was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse Wnt-8b is a 40-50 kDa secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329 aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98%, and 97% identical to mature rat, canine, and human Wnt-8b, respectively.
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