Mouse Wnt-8b Antibody (341502)

R&D Systems | Catalog # MAB33671

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 341502
View all Wnt-8b Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Wnt‑8b
Trp22-Glu52, Leu195-Arg269
Accession # Q9WUD6

Specificity

Detects mouse Wnt-8b in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Wnt-2, -7a, -7b,-9a, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, or -9b is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Wnt-8b Antibody (341502)

Detection of Mouse Wnt‑8b antibody by Western Blot.

Detection of Mouse Wnt‑8b by Western Blot.

Western blot shows lysates of KATO-III human gastric carcinoma cell line. PVDF membrane was probed with 5 µg/mL Rat Anti-Mouse Wnt-8b Monoclonal Antibody (Catalog # MAB33671) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant mouse Wnt-8b (20 ng) was included. A specific band for native Wnt-8b was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

Applications for Mouse Wnt-8b Antibody (341502)

Application
Recommended Usage

Western Blot

5 µg/mL
Sample: KATO-III human gastric carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-8b

Mouse Wnt-8b is a 40-50 kDa secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329 aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98%, and 97% identical to mature rat, canine, and human Wnt-8b, respectively.

Long Name

Wingless-type MMTV Integration Site Family, Member 8b

Alternate Names

Wnt8b

Entrez Gene IDs

7479 (Human); 22423 (Mouse); 293990 (Rat)

Gene Symbol

WNT8B

UniProt

Q9WUD6

Additional Wnt-8b Products

Product Documents for Mouse Wnt-8b Antibody (341502)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Wnt-8b Antibody (341502)

For research use only

Related Research Areas

Wnt Ligands (Wnts)

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