Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ZIP4H/Tex11 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse ZIP4H/Tex11
Met768-Leu947
Accession # Q14AT2

Specificity

Detects mouse ZIP4H/Tex11 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse ZIP4H/Tex11 Antibody

Detection of Mouse ZIP4H/Tex11 antibody by Western Blot.

Detection of Mouse ZIP4H/Tex11 by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line either mock transfected, transfected with human ZIP4H, or transfected with mouse ZIP4H. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse ZIP4H/Tex11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5627) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ZIP4H/Tex11 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse ZIP4H/Tex11 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: 293T human embryonic kidney cell line either mock transfected, transfected with human ZIP4H, or transfected with mouse ZIP4H

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ZIP4H/Tex11

ZIP4 homolog (ZIP4H), also known as testis expressed sequence 11 (Tex11) is a 947 amino acid (aa) X chromosome-encoded protein that is selectively expressed in primordial male germ cells. It associates with Nbs1 of the Mre11 protein complex which results in localization to areas of synaptonemal contact between meiotic chromosomes. During meiosis, ZIP4H promotes sister chromatid synapsis and crossover in response to programmed double stranded DNA breaks. ZIP4H deficiency leads to increased spermatocyte apoptosis and male sterility. Alternate splicing in mouse generates an additional isoform with a substitution (aa 691‑707) and truncation (aa 708‑947) at the C-terminus. Within aa 768‑947, mouse ZIP4H shares 44% and 73% aa sequence identity with human and rat ZIP4H, respectively.

Long Name

Zinc Transporter Protein 4 Homolog

Alternate Names

Tex11, TGC1, TSGA3

Entrez Gene IDs

56159 (Human); 83558 (Mouse); 501588 (Rat)

Gene Symbol

TEX11

UniProt

Q14AT2

Additional ZIP4H/Tex11 Products

Product Documents for Mouse ZIP4H/Tex11 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse ZIP4H/Tex11 Antibody

For research use only

Related Research Areas

Primordial and Mature Germ Cells

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