Mre11 Antibody (12D7) - Azide and BSA Free

Novus Biologicals | Catalog # NB100-473

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Functional, Proximity Ligation Assay, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 12D7

Format

Azide and BSA Free
View all Mre11 Primary Antibodies »

Product Specifications

Immunogen

Amino acids 182-582 of Mre11 expressed in E. coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Mre11 Antibody (12D7) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Mre11 Antibody (12D7) [NB100-473]

Immunocytochemistry/ Immunofluorescence: Mre11 Antibody (12D7) [NB100-473]

Immunocytochemistry/Immunofluorescence: Mre11 Antibody (12D7) [NB100-473] - Human primary cells stained with Mre11 antibody at a dilution of 1:60. cells were fixed in 1:1 Acetone/Methanol. ICC/IF image submitted by a verified customer review.
Knockdown Validated: Mre11 Antibody (12D7) [NB100-473]

Western Blot: Mre11 Antibody (12D7) [NB100-473]

Western Blot: Mre11 Antibody (12D7) [NB100-473] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] diluted at 1:500. HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. A band appears at the theoretical molecular weight of 80 kDa.
Western Blot: Mre11 Antibody (12D7) [NB100-473]

Western Blot: Mre11 Antibody (12D7) [NB100-473]

Western Blot: Mre11 Antibody (12D7) [NB100-473] - Mre11 antibody [12D7] detects Mre11 protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug whole cell extract of human Mre11-transfected 293T cells. 7.5% SDS-PAGE. Mre11 antibody [12D7] (NB100-473). HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. Bands are prominent at the theoretical molecular weight of 80 kDa.
Immunocytochemistry/ Immunofluorescence: Mre11 Antibody (12D7) [NB100-473]

Immunocytochemistry/ Immunofluorescence: Mre11 Antibody (12D7) [NB100-473]

Immunocytochemistry/Immunofluorescence: Mre11 Antibody (12D7) [NB100-473] - Analysis of Mre11 antibody (12D7) on lymphoblastoid cells. Dilution 1:200. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Mre11 Antibody (12D7) [NB100-473]

Immunocytochemistry/ Immunofluorescence: Mre11 Antibody (12D7) [NB100-473]

Immunocytochemistry/Immunofluorescence: Mre11 Antibody (12D7) [NB100-473] - Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Mre11 stained by Mre11 antibody [12D7] (NB100-473).Blue: Hoechst 33342 staining.Scale bar= 10 um.
Immunocytochemistry/ Immunofluorescence: Mre11 Antibody (12D7) [NB100-473]

Immunocytochemistry/ Immunofluorescence: Mre11 Antibody (12D7) [NB100-473]

Immunocytochemistry/Immunofluorescence: Mre11 Antibody (12D7) [NB100-473] - Mouse fibroblasts stained with Mre11 Antibody (12D7). ICC/IF image submitted by a verified customer review.
Mre11 Antibody (12D7)

Western Blot: Mre11 Antibody (12D7) [NB100-473] -

Western Blot: Mre11 Antibody (12D7) [NB100-473] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (NB100-473) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.

Applications for Mre11 Antibody (12D7) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:100

Western Blot

1:500 - 1:3000
Application Notes
Mre11 antibody validated for ICC/IF and WB from verified customer reviews.

Reviewed Applications

Read 4 reviews rated 5 using NB100-473 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mre11

Mre11 (double-strand break repair protein MRE11A) is an important component of MRN/X complex (Mre11, Rad50, and Nbs1/Xrs2), playing a central role in double-strand break (DSB) repair, DNA recombination, telomere integrity maintenance, meiotic cell division, and viral infection. Mre11 and Rad50 comprise the catalytic component of the MRN complex with Rad50 involved in ATP hydrolysis and Mre11 functioning as a nuclease having both single-strand endonuclease and double-strand-specific 3'-5' exonuclease activity (1). The MRN complex is also involved in DNA damage signaling via activation of ATM kinase and in telomeres, where it may modulate t-loop formation (2). In addition, Mre11 is a component of the BASC complex (BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN) and interacts with DCLRE1C/Artemis as well as DCLRE1B/Apollo (3).

The MRN assembles as a hetero-hexamer consisting of 2 RAD50 subunits, 2 MRE11 nucleases, and 2 NBS1 subunits, specific to eukaryotes. Mre11 is highly conserved with the N-terminal region of the human protein sharing approximately 50% amino acid sequence identity with the yeast ortholog. Human Mre11 has 3 known isoforms with the predominant isoform having a theoretical molecular weight of 80.6kDa. This nuclear localized protein has high expression in proliferating tissues. Defects in Mre11 can lead to genomic instability, telomere shortening, aberrant meiosis, hypersensitivity to DNA damage, and ataxia telangiectasia-like disorder (ATLD). MRN expression has been associated with the prognosis of cancer and MRN mutants have been linked to cancer susceptibility in ovarian cancer and glioma (4, 5).

References

1. Paull TT. (2018) 20 Years of Mre11 Biology: No End in Sight. Mol Cell. 71(3):419-427. PMID: 30057197

2. Syed A, Tainer JA. (2018) The MRE11-RAD50-NBS1 Complex Conducts the Orchestration of Damage Signaling and Outcomes to Stress in DNA Replication and Repair. Annu Rev Biochem. 87:263-294. PMID: 29709199

3. Wang Y, Cortez D, Yazdi P, Neff N, Elledge SJ, Qin J. (2000) BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev. 14(8):927-39. PMID: 10783165

4. Kim JH, Grosbart M, Anand R, Wyman C, Cejka P, Petrini JHJ. (2017) The Mre11-Nbs1 Interface Is Essential for Viability and Tumor Suppression. Cell Rep. 18(2):496-507. PMID: 28076792

5. Bian L, Meng Y, Zhang M, Li D. (2019) MRE11-RAD50-NBS1 complex alterations and DNA damage response: implications for cancer treatment. Mol Cancer. 18(1):169. PMID: 31767017

Long Name

Meiotic Recombination 11 Homolog A

Alternate Names

MRE11A

Entrez Gene IDs

4361 (Human)

Gene Symbol

MRE11

UniProt

P49959

Additional Mre11 Products

Product Documents for Mre11 Antibody (12D7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Mre11 Antibody (12D7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Mre11 Antibody (12D7) - Azide and BSA Free

Customer Reviews for Mre11 Antibody (12D7) - Azide and BSA Free (4)

5 out of 5
4 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Mre11 Antibody (12D7) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 of 4 reviews Showing All
Filter By:
Clear Filters X
  • Mre11 Antibody (12D7)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: lymphoblastoid cells
    Species: Human
    Verified Customer | Posted 06/28/2022
    staining of lymphoblastoid cells
    Immunocytochemistry dilution 1:200.
    Mre11 Antibody (12D7) - Azide and BSA Free NB100-473
  • Mre11 Antibody (12D7)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: fibroblasts
    Species: Mouse
    Verified Customer | Posted 12/04/2021
    Mre11 Antibody
    Mre11 Antibody (12D7) - Azide and BSA Free NB100-473
  • Mre11 Antibody (12D7)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: primary human cells
    Species: Human
    Verified Customer | Posted 12/22/2016
    Primary cells fixed in methanol:acetone (1:1) and stained with MRE11 (12D7) 1:60
    Mre11 Antibody (12D7) - Azide and BSA Free NB100-473
  • Name: De-identified L
    Application: Western Blot
    Sample Tested: HEK293 whole cell lysates
    Species: Human
    Verified Customer | Posted 04/30/2014
    Detection of human MRE11 using 12D7 (NB100-473) in HEK293 whole cell lysates. Dilution 1:1000.
    Mre11 Antibody (12D7) - Azide and BSA Free NB100-473

There are no reviews that match your criteria.

Showing  1 - 4 of 4 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Mre11 Antibody (12D7) - Azide and BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: Could you recommend an optimal Mre11 positive control for Western blot among your products?

    A: NBL1-13219 would be the best product since it is intended for this purpose.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies