Musashi-1 Antibody (3F2) - Azide and BSA Free
Novus Biologicals | Catalog # H00004440-M04
![Western Blot: Musashi-1 Antibody (3F2) [H00004440-M04]](https://resources.rndsystems.com/images/products/Musashi-1-Antibody-3F2-Western-Blot-H00004440-M04-img0008.jpg "Western Blot: Musashi-1 Antibody (3F2) [H00004440-M04]")
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Key Product Details
Species Reactivity
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3F2
Format
Azide and BSA Free
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Product Specifications
Immunogen
MSI1 (NP_002433, 1 a.a. ~ 87 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. METDAPQPGL ASPDSPHDPC KMFIGGLSWQ TTQEGLREYF GQFGE VKECL VMRDPLTKRS RGFGFVTFMD QAGVDKVLAQ SRHELDS
Marker
Neuronal Stem Cell Marker
Specificity
MSI1 (3F2)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Musashi-1 Antibody (3F2) - Azide and BSA Free
Western Blot: Musashi-1 Antibody (3F2) [H00004440-M04]
Western Blot: Musashi-1 Antibody (3F2) [H00004440-M04] - Analysis of MSI1 expression in IMR-32 (Cat # L008V1).![Immunocytochemistry/ Immunofluorescence: Musashi-1 Antibody (3F2) [H00004440-M04]](https://resources.rndsystems.com/images/products/Musashi-1-Antibody-3F2-Immunocytochemistry-Immunofluorescence-H00004440-M04-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Musashi-1 Antibody (3F2) [H00004440-M04]")
Immunocytochemistry/ Immunofluorescence: Musashi-1 Antibody (3F2) [H00004440-M04]
Immunocytochemistry/Immunofluorescence: Musashi-1 Antibody (3F2) [H00004440-M04] - Analysis of monoclonal antibody to MSI1 on HeLa cell. Antibody concentration 10 ug/ml![Immunohistochemistry-Paraffin: Musashi-1 Antibody (3F2) [H00004440-M04]](https://resources.rndsystems.com/images/products/Musashi-1-Antibody-3F2-Immunohistochemistry-Paraffin-H00004440-M04-img0007.jpg "Immunohistochemistry-Paraffin: Musashi-1 Antibody (3F2) [H00004440-M04]")
Immunohistochemistry-Paraffin: Musashi-1 Antibody (3F2) [H00004440-M04]
Immunohistochemistry-Paraffin: Musashi-1 Antibody (3F2) [H00004440-M04] - Analysis of monoclonal antibody to MSI1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml![ELISA: Musashi-1 Antibody (3F2) [H00004440-M04]](https://resources.rndsystems.com/images/products/Musashi-1-Antibody-3F2-ELISA-H00004440-M04-img0004.jpg "ELISA: Musashi-1 Antibody (3F2) [H00004440-M04]")
ELISA: Musashi-1 Antibody (3F2) [H00004440-M04]
ELISA: Musashi-1 Antibody (3F2) [H00004440-M04] - Detection limit for recombinant GST tagged MSI1 is approximately 0.3ng/ml as a capture antibody.Applications for Musashi-1 Antibody (3F2) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. IT has been used for IF, IHC-P, and ELISA. FACS usage reported in scientific literature (PMID: 23818760).
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Musashi-1
Alternate Names
MSI1, Musashi1
Entrez Gene IDs
4440 (Human)
Gene Symbol
MSI1
OMIM
603328 (Human)
UniProt
Additional Musashi-1 Products
Product Documents for Musashi-1 Antibody (3F2) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Musashi-1 Antibody (3F2) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Musashi-1 Antibody (3F2) - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
