N-Cadherin Antibody (CL3716) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44495

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3716

Format

Azide and BSA Free
View all N-Cadherin Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P19022, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for N-Cadherin Antibody (CL3716) - Azide and BSA Free

N-Cadherin Antibody (CL3716) - Azide and BSA Free Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP3-44495]

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP3-44495]

Analysis in human heart muscle and skin tissues using NBP3-44495 antibody. Corresponding N-Cadherin RNA-seq data are presented for the same tissues.
N-Cadherin Antibody (CL3716) - Azide and BSA Free Western Blot: N-Cadherin Antibody [NBP3-44495]

Western Blot: N-Cadherin Antibody [NBP3-44495]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line U-251 MG
N-Cadherin Antibody (CL3716) - Azide and BSA Free Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP3-44495]

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP3-44495]

Staining of human heart muscle shows strong positivity in the intercalated discs of cardiomyocytes.
N-Cadherin Antibody (CL3716) - Azide and BSA Free Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP3-44495]

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP3-44495]

Staining of human cerebral cortex shows moderate positivity in neuropil.
N-Cadherin Antibody (CL3716) - Azide and BSA Free Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP3-44495]

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP3-44495]

Staining of human liver cancer (hepatocellular carcinoma) shows moderate to strong membranous positivity in tumor cells.
N-Cadherin Antibody (CL3716) - Azide and BSA Free Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP3-44495]

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP3-44495]

Staining of human skin shows no positivity in epidermal cells as expected.
N-Cadherin Antibody (CL3716) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody [NBP3-44495]

Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody [NBP3-44495]

Staining of U-251 cells using the Anti-N-Cadherin monoclonal antibody).
N-Cadherin Antibody (CL3716) - Azide and BSA Free Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP3-44495]

Immunohistochemistry-Paraffin: N-Cadherin Antibody [NBP3-44495]

Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.
N-Cadherin Antibody (CL3716) - Azide and BSA Free Western Blot: N-Cadherin Antibody [NBP3-44495]

Western Blot: N-Cadherin Antibody [NBP3-44495]

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-N-Cadherin antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Applications for N-Cadherin Antibody (CL3716) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: N-Cadherin

N-Cadherin, also referred to as Neural Cadherin (NCAD) or Cadherin-2 (CHD2), is a 130 kDa protein that is a member of the calcium-dependent adhesion molecule family of classical (type I) cadherins (1-4). Under the CDH2 gene, human N-cadherin is synthesized as a 906 amino acid protein with a theoretical molecular weight of 99.8 kDa (5). The N-cadherin protein structure is similar to other classical type I cadherins including epithelial (E-) cadherin and placental (P-) cadherin (1,2). N-cadherin consists of a 25 amino acid (aa) N-terminal signal peptide and 134 aa pro-peptide, a 565 aa extracellular domain (ECD) with five cadherin repeats, a 21 aa transmembrane segment, and a 161 aa cytoplasmic domain (1-3,5). The ECD of N-cadherin monomers is responsible for homotypic binding through either cis or trans adhesion (2,3).

N-cadherin is expressed on multiple cell types but is most highly expressed by mesenchymal cells and neural tissue (2). Functionally, N-cadherin has a number of roles including maintaining structural integrity and adhesion, cell signaling, and formation of neuronal synapses and the vascular wall (2). The cytoplasmic tail interacts with beta-catenin which then binds with alpha-catenin, forming the cadherin-catenin adhesion complex, an important component of adhesions junctions (1-3). Given its role in adhesion, N-cadherin serves as an indicator of epithelial-to-mesenchymal transition (EMT) (1-4). The loss of E-cadherin during EMT corresponds with an increase in N-cadherin expression (1-4). This "cadherin-switch" is associated with increased migratory and invasive behavior observed in tumor progress (1-4). Proteases including activity of a disintegrin and metalloprotease 10 (ADAM10), matrix metalloproteinases (MMPs), caspase 3, presenilin, and calpain can cleave N-cadherin as a mechanism for regulating Wnt/beta-catenin signaling and inducing oncogenic signals (3,4). In addition to its expression in solid tumors, N-cadherin has been indicated in hematological disorders such as leukemia and multiple myeloma (1). N-cadherin antagonists are currently being studied as potential therapeutics for a variety of cancer studies (1-2).

References

1. Mrozik, K. M., Blaschuk, O. W., Cheong, C. M., Zannettino, A., & Vandyke, K. (2018). N-cadherin in cancer metastasis, its emerging role in haematological malignancies and potential as a therapeutic target in cancer. BMC Cancer. https://doi.org/10.1186/s12885-018-4845-0

2. Loh, C. Y., Chai, J. Y., Tang, T. F., Wong, W. F., Sethi, G., Shanmugam, M. K., Chong, P. P., & Looi, C. Y. (2019). The E-Cadherin and N-Cadherin Switch in Epithelial-to-Mesenchymal Transition: Signaling, Therapeutic Implications, and Challenges. Cells. https://doi.org/10.3390/cells8101118

3. Derycke, L. D., & Bracke, M. E. (2004). N-cadherin in the spotlight of cell-cell adhesion, differentiation, embryogenesis, invasion and signalling. The International Journal of Developmental Biology. https://doi.org/10.1387/ijdb.041793ld

4. Yu, W., Yang, L., Li, T., & Zhang, Y. (2019). Cadherin Signaling in Cancer: Its Functions and Role as a Therapeutic Target. Frontiers in Oncology. https://doi.org/10.3389/fonc.2019.00989

5. Unitprot (P1903)

Long Name

Neural Cadherin

Alternate Names

Cadherin-2, CD325, CDH2, NCadherin

Gene Symbol

CDH2

Additional N-Cadherin Products

Product Documents for N-Cadherin Antibody (CL3716) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for N-Cadherin Antibody (CL3716) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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