NAIP Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-77196
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, E. coli
Cited:
Bacteria - Escherichia coli
Predicted:
Mouse (92%). Backed by our 100% Guarantee.
Applications
Validated:
Immunomicroscopy, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, SDS-Page
Cited:
Immunomicroscopy, Western Blot, Immunoprecipitation, SDS-Page
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
NAIP antibody was raised against a synthetic peptide corresponding to 13 amino acids at the C-terminus of human NAIP. The immunogen is located within amino acids 1200 - 1250 of NAIP.
Reactivity Notes
E. coli reactivity reported in scientific literature (PMID: 31551961).
Specificity
Minor, lower molecular weight bands may represent alternately spliced forms.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
155 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NAIP Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: NAIP Antibody - BSA Free [NBP1-77196]
Immunocytochemistry/Immunofluorescence: NAIP Antibody [NBP1-77196] - Confocal microscopy analysis of NAIP expression in THP-1m with or without CFT073 stimulation. NLRC4 and NAIP expression in THP-1m, 6 h post stimulation with or without (mock) CFT073 was examined with confocal microscopy analysis using anti-NAIP antibody. Alexa fluor 488 (green) was used to detect anti-NAIP antibody. Nucleus of cells were stained with DAPI (blue). Results are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fmicb.2019.02020/full) licensed under a CC-BY license.Western Blot: NAIP AntibodyBSA Free [NBP1-77196]
Western Blot: NAIP Antibody [NBP1-77196] - Analysis of NLRP3, NLRC4, NAIP, AIM2, ASC, Caspase-4, Caspase-1, B-actin and GAPDH protein expression by immunoblotting. (A) Represents the immunoblotting results of NLRP3, NLRC4, NAIP, Caspase-1, GAPDH of UPEC infected UTI patients and controls group. Scattered plots showing individual densitometric values (IDV) of NAIP (D). Results were expressed as average densitometric ratio in patients and controls group +/- SD. P-value is ***p = 0.0001 and n.s, non-significant. anti-NLRP3 antibody (NBP1-77080), anti-NLRC4 antibody (NBP2-41124), anti-Caspase-1 antibody (NBP1-45433). Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fmicb.2019.02020/full) licensed under a CC-BY license.Western Blot: NAIP Antibody - BSA Free [NBP1-77196] -
Western Blot: NAIP Antibody - BSA Free [NBP1-77196] - Western blot analysis of NAIP in human tonsil tissue lysate with NAIP antibody at 1 ug/mL.Immunocytochemistry/ Immunofluorescence: NAIP Antibody - BSA Free [NBP1-77196] -
Immunocytochemistry/ Immunofluorescence: NAIP Antibody - BSA Free [NBP1-77196] - Immunofluorescence of NAIP in A549 cells with NAIP antibody at 20 ug/mL.Immunocytochemistry/ Immunofluorescence: NAIP Antibody - BSA Free [NBP1-77196] -
Immunocytochemistry/ Immunofluorescence: NAIP Antibody - BSA Free [NBP1-77196] - Immunocytochemistry of NAIP in A549 cells with NAIP antibody at 10 ug/mL.Applications for NAIP Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
10-20 ug/mL
Western Blot
0.5-1 ug/ml
Application Notes
Use in SDS- Page, Immunoprecipitation and Immunomicroscopy reported in scientific literature (PMID:31551961). Molecular Weight: Predicted: 137, 154 kDa; Observed: 155 kDa
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NAIP
Long Name
Neuronal Apoptosis Inhibitor Protein
Alternate Names
BIRC1, NLRB1
Gene Symbol
NAIP
UniProt
Additional NAIP Products
Product Documents for NAIP Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NAIP Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for NAIP Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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