NCOA2 Antibody

Novus Biologicals | Catalog # NB100-1756

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Chimpanzee (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all NCOA2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1400 and the C-terminus (residue 1464) of human Nuclear Receptor Coactivator 2 using the numbering given in entry NP_006531.1 (GeneID 10499).

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Gorilla, White-tufted-ear Marmoset and Northern White-cheeked Gibbon. Mouse reactivity reported in scientific literature (PMID: 26465008).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCOA2 Antibody

Immunocytochemistry/ Immunofluorescence: NCOA2 Antibody [NB100-1756]

Immunocytochemistry/ Immunofluorescence: NCOA2 Antibody [NB100-1756]

NCOA2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1756-img0005.jpg
Immunohistochemistry: NCOA2 Antibody [NB100-1756]

Immunohistochemistry: NCOA2 Antibody [NB100-1756]

Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Sample : FFPE section of prostate adenocarcinoma. Antibody : Affinity purified rabbit anti-NCOA2/SRC2 used at a dilution of 1:100. Detection : DAB
Immunocytochemistry/ Immunofluorescence: NCOA2 Antibody [NB100-1756]

Immunocytochemistry/ Immunofluorescence: NCOA2 Antibody [NB100-1756]

NCOA2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1756-img0004.jpg
Immunohistochemistry: NCOA2 Antibody [NB100-1756]

Immunohistochemistry: NCOA2 Antibody [NB100-1756]

Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Samples: FFPE sections of human breast adenocarcinoma (upper image) and prostate adenocarcinoma (lower image). Antibody: Affinity purified rabbit anti-SRC2 NB100-1756 used at a dilution of 1:100. Detection: DAB
Immunohistochemistry: NCOA2 Antibody [NB100-1756]

Immunohistochemistry: NCOA2 Antibody [NB100-1756]

Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Sample : FFPE section of human breast adenocarcinoma. Antibody : Affinity purified rabbit anti-NCOA2/SRC2 used at a dilution of 1:250. Detection : DAB
NCOA2 Antibody

Immunocytochemistry/ Immunofluorescence: NCOA2 Antibody [NB100-1756] -

Immunocytochemistry/ Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 & SRC-2. A–P, Representative images taken from vehicle control wt mice (A–D) & estradiol-treated wt mice (E–H), PRBKO mice (that express PR-A only; I–L), & PRAKO mice (that express PR-B only; M–P). Insets show the magnified image of an area within the small square box. Magnification: images, 400×; insets, 630×. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26465008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
NCOA2 Antibody

Immunocytochemistry/ Immunofluorescence: NCOA2 Antibody [NB100-1756] -

Immunocytochemistry/ Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 & SRC-2. A–P, Representative images taken from vehicle control wt mice (A–D) & estradiol-treated wt mice (E–H), PRBKO mice (that express PR-A only; I–L), & PRAKO mice (that express PR-B only; M–P). Insets show the magnified image of an area within the small square box. Magnification: images, 400×; insets, 630×. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26465008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
NCOA2 Antibody

Immunocytochemistry/ Immunofluorescence: NCOA2 Antibody [NB100-1756] -

Immunocytochemistry/ Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 & SRC-2. A–P, Representative images taken from vehicle control wt mice (A–D) & estradiol-treated wt mice (E–H), PRBKO mice (that express PR-A only; I–L), & PRAKO mice (that express PR-B only; M–P). Insets show the magnified image of an area within the small square box. Magnification: images, 400×; insets, 630×. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26465008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for NCOA2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:500
Application Notes
Likely to work in frozen sections. Although not tested this antibody may be useful in ICC/IF. *The investigator should determine the optimal working dilution for a specific application. Use in Western blot reported in scientific literature (PMID: 26465008).

Reviewed Applications

Read 1 review rated 4 using NB100-1756 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.25 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NCOA2

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is Steroid Receptor Coactivator-2 (SRC2).

Long Name

Nuclear Receptor Co-activator 2

Alternate Names

bHLHe75, HTIF2, KAT13C, TIF2

Entrez Gene IDs

10499 (Human)

Gene Symbol

NCOA2

UniProt

Q15596 (Human)

Additional NCOA2 Products

Product Documents for NCOA2 Antibody

Certificate of Analysis

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Product Specific Notices for NCOA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for NCOA2 Antibody (1)

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  • NCOA2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Brain (hypothalamus) tissue
    Species: Mouse and Rat
    Verified Customer | Posted 10/06/2016
    NCOA2 Antibody NB100-1756

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