Neprilysin/CD10 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15771

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Predicted:

Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Neprilysin/CD10 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Neprilysin/CD10. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (89%), Rabbit (88%), Bovine (86%).

Localization

Cell membrane; Single-pass type II membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Neprilysin/CD10 Antibody - BSA Free

Western Blot: Neprilysin/CD10 Antibody [NBP2-15771]

Western Blot: Neprilysin/CD10 Antibody [NBP2-15771]

Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Neprilysin/CD10 Antibody [NBP2-15771]

Western Blot: Neprilysin/CD10 Antibody [NBP2-15771]

Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - A. 50 ug rat kidney lysate/extract 7.5 % SDS-PAGECD10 antibody [N2C1], Internal dilution: 1:500
Western Blot: Neprilysin/CD10 Antibody [NBP2-15771]

Western Blot: Neprilysin/CD10 Antibody [NBP2-15771]

Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Neprilysin/CD10 Antibody - BSA Free

Western Blot: Neprilysin/CD10 Antibody - BSA Free [NBP2-15771] -

MRPL49 protein is a substrate of neprilysin. (A) Representative WB analysis showing the rescue of MRPL49 levels, after neprilysin knock-down (KD). siCTRL: pool of control siRNAs. siNEP: pool of neprilysin-silencing siRNAs. (B) Relative fold-change in the levels of neprilysin protein in both controls (siCTRL) and neprilysin-silenced (siNEP) cells upon DA treatment, showing the effectiveness of the KD. Three biological replicates (n=3). Error bars: SEM. Statistical analysis performed by two-way ANOVA, to assess the effects of both “KD" (siCTRL vs. siNEP) and “treatment" (CTRL vs. DA). The only significant source of variation was “KD" (p = 0.004; F = 15.98). (C) Relative fold-change in the levels of MRPL49 protein in siCTRL and siNEP cells upon DA treatment, showing the rescue of the levels of MRPL49 upon neprilysin KD. Three biological replicates (n=3). Error bars: SEM. Statistical analysis performed by two-way ANOVA. Both “treatment" (p = 0.0057; F = 14.02) and “interaction" (p = 0.040; F = 5.98) resulted to be significant sources of variation. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31417398), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Neprilysin/CD10 Antibody - BSA Free

Western Blot: Neprilysin/CD10 Antibody - BSA Free [NBP2-15771] -

Neprilysin expression and localization in SH-SY5Y cells. (A) Representative WB analysis showing the presence of neprilysin in SH-SY5Y cells (T: total extracts) and its enrichment in mitochondrial isolates (M). (B) Relative fold-change in the levels of neprilysin in both total (T) and mitochondrial (M) extracts upon DA treatment, showing the significant enrichment of neprilysin in the mitochondrial fraction. Normalization was based on total protein amount per lane. Three biological replicates (n=3). Error bars: SEM. Statistical analysis performed by two-way ANOVA, to assess the effects of both “localization" (T vs. M) and “treatment" (CTRL vs. DA). The only significant source of variation was “localization" (p = 0.0002; F = 40.55). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31417398), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Neprilysin/CD10 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID: 30768735)

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 3 using NBP2-15771 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Neprilysin/CD10

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq]

Alternate Names

CALLA, CD10, Enkephalinase, Leu-19, MME, Neutral Endopeptidase 24.11, NKH1

Gene Symbol

MME

UniProt

P08473

Additional Neprilysin/CD10 Products

Product Documents for Neprilysin/CD10 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Neprilysin/CD10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Neprilysin/CD10 Antibody - BSA Free

Customer Reviews for Neprilysin/CD10 Antibody - BSA Free (1)

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  • Neprilysin/CD10 Antibody
    Name: David Krull
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human skin
    Species: Human
    Verified Customer | Posted 12/08/2016
    Neprilysin/CD10 Antibody - BSA Free NBP2-15771

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