Nestin Antibody (10C2) - Azide and BSA Free

Flow Cytometry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869]

Flow Cytometry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - An intracellular stain was performed on SH-SY5Y cells with Nestin [10C2] Antibody NB300-266C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to DyLight 650.

Immunocytochemistry/ Immunofluorescence: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869]

Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - Analysis of formalin fixed, 0.1% Triton X-100 permeabilized F9 cells (Mouse teratocarcinoma) using Nestin antibody (clone 10C2) at 1:50 dilution. The primary antibody was detected using Alexa Fluor 488 labeled donkey anti-mouse IgG (H+L) cross-adsorbed secondary antibody (green) and nuclei were counterstained with DAPI (blue). An isotype control set was inclubated with mouse IgG1 isotype control antibody and the above mentioned secondary antibody. Nestin antibody (clone 10C2) generated a specific membrane-cytoplasmic staining of Nestin while the isotype control samples did not show any staining under same assay conditions. Image from the standard format of this antibody.

Immunocytochemistry/ Immunofluorescence: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869]

Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - Visualization of Nestin in PC-3 cells using NB300-266. Courtesy of Dolnick Lab, Roswell Park Cancer Institute. Image from the standard format of this antibody.

Flow Cytometry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869]

Flow Cytometry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - An intracellular stain was performed on HeLa cells with Nestin Antibody (10C2)NB300-266PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.

Flow Cytometry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869]

Flow Cytometry: Nestin Antibody (10C2) - Azide and BSA Free [NBP2-80869] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Nestin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used. Image from the standard format of this antibody.