Nestin Antibody (CL0197) - BSA Free

Novus Biologicals | Catalog # NBP2-59775

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0197

Format

BSA Free
View all Nestin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P48681, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Nestin Antibody (CL0197) - BSA Free

Western Blot: Nestin Antibody (CL0197) [NBP2-59775]

Western Blot: Nestin Antibody (CL0197) [NBP2-59775]

Western Blot: Nestin Antibody (CL0197) [NBP2-59775] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: Nestin Antibody (CL0197) [NBP2-59775]

Immunocytochemistry/ Immunofluorescence: Nestin Antibody (CL0197) [NBP2-59775]

Immunocytochemistry/Immunofluorescence: Nestin Antibody (CL0197) [NBP2-59775] - Staining of RH-30 cells using the Anti-NES monoclonal antibody, showing specific staining in intermediate filaments in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775]

Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775]

Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775] - Staining of human kidney shows strong positivity in renal glomeruli.
Western Blot: Nestin Antibody (CL0197) [NBP2-59775]

Western Blot: Nestin Antibody (CL0197) [NBP2-59775]

Western Blot: Nestin Antibody (CL0197) [NBP2-59775] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-NES monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775]

Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775]

Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775] - Staining of human cerebral cortex shows strong immunoreactivity in the endothelial cells.

Applications for Nestin Antibody (CL0197) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nestin

Nestin is a Class VI intermediate filament expressed in the developing central nervous system in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. Because if it's roles in angiogenisis and tumorgenesis, Nestin antibodies are useful cancer markers.

Although it is often used as a marker for proliferating and migrating cells very little is known about Nestin's function or regulation.

Alternate Names

NES

Gene Symbol

NES

Additional Nestin Products

Product Documents for Nestin Antibody (CL0197) - BSA Free

Certificate of Analysis

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Product Specific Notices for Nestin Antibody (CL0197) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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