Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Goat IgG
View all Nestin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat Nestin
Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)
Accession # EDM00749

Specificity

Detects rat Nestin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Nestin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Nestin Antibody [DyLight 405]

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Nestin

Nestin is a Class VI intermediate filament expressed in the developing central nervous system in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. Because if it's roles in angiogenisis and tumorgenesis, Nestin antibodies are useful cancer markers.

Although it is often used as a marker for proliferating and migrating cells very little is known about Nestin's function or regulation.

Alternate Names

NES

Gene Symbol

NES

Additional Nestin Products

Product Documents for Nestin Antibody [DyLight 405]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Nestin Antibody [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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