hh Rat Nestin Antibody AF2736: R&D Systems

Rat Nestin Antibody

(5 citations)   
  • Species Reactivity
    Rat
  • Specificity
    Detects rat Nestin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Nestin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant rat Nestin
    Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)
    Accession # EDM00749
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Rat Nestin
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of rat embyo (E15)
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry

Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed rat cortical stem cells using 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry

beta ‑III Tubulin and Nestin in Rat Cortical Stem Cells. beta ‑III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using 5 µg/mL neuron-specific Mouse beta ‑III Tubulin Monoclonal (clone TuJ‑1) Antibody (Catalog # MAB1195) and 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta‑III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for Nestin using the NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Tissue was counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry

Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed rat cortical stem cells using Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nestin
Nestin is a 240 kDa class VI intermediate filament protein that was first identified in neuroepithelial stem cells (1, 2). It has been used extensively as a marker to identify central nervous system (CNS) stem cell populations. In addition, Nestin has been found to be a marker for multi-lineage progenitor cells. Over the region used for immunization, rat Nestin shares 76% amino acid sequence homology with the mouse protein.
  • References:
    1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
    2. Lendahl, U. et al. (1990) Cell 60:585.
  • Entrez Gene IDs:
    10763 (Human); 18008 (Mouse); 25491 (Rat)
  • Alternate Names:
    FLJ21841; NES; Nestin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Nestin-positive microglia in adult rat cerebral cortex.
    Authors: Takamori Y, Mori T, Wakabayashi T, Nagasaka Y, Matsuzaki T, Yamada H
    Brain Res., 2009;1270(0):10-8.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Frozen
  2. Pten deletion in adult neural stem/progenitor cells enhances constitutive neurogenesis.
    Authors: Gregorian C, Nakashima J, Le Belle J, Ohab J, Kim R, Liu A, Smith KB, Groszer M, Garcia AD, Sofroniew MV, Carmichael ST, Kornblum HI, Liu X, Wu H
    J. Neurosci., 2009;29(6):1874-86.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Mechanisms of murine lacrimal gland repair after experimentally induced inflammation.
    Authors: Zoukhri D, Fix A, Alroy J, Kublin CL
    Invest. Ophthalmol. Vis. Sci., 2008;49(10):4399-406.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Vasopressin mediates mitogenic responses to adrenalectomy in the rat anterior pituitary.
    Authors: Subburaju S, Aguilera G
    Endocrinology, 2007;148(7):3102-10.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.
    Authors: De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD
    Biotechnol. Bioeng., 2007;97(6):1617-25.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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