Rat Nestin Antibody

Catalog # Availability Size / Price Qty
AF2736
AF2736-SP
Rat Nestin Antibody in Data
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Product Details
Citations (7)
FAQs
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Rat Nestin Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Nestin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Nestin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat Nestin
Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)
Accession # EDM00749
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat Nestin
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of rat embyo (E15)
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry Nestin in Rat Cortical Stem Cells. View Larger

Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed rat cortical stem cells using 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry beta ‑III Tubulin and Nestin in Rat Cortical Stem Cells. View Larger

beta ‑III Tubulin and Nestin in Rat Cortical Stem Cells. beta -III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using 5 µg/mL neuron-specific Mouse beta -III Tubulin Monoclonal (clone TuJ-1) Antibody (Catalog # MAB1195) and 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for Nestin using the NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Tissue was counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Nestin in Rat Cortical Stem Cells. View Larger

Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed rat cortical stem cells using Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nestin

Nestin is a 240 kDa class VI intermediate filament protein that was first identified in neuroepithelial stem cells (1, 2). It has been used extensively as a marker to identify central nervous system (CNS) stem cell populations. In addition, Nestin has been found to be a marker for multi-lineage progenitor cells. Over the region used for immunization, rat Nestin shares 76% amino acid sequence homology with the mouse protein.

References
  1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
  2. Lendahl, U. et al. (1990) Cell 60:585.
Entrez Gene IDs
10763 (Human); 18008 (Mouse); 25491 (Rat)
Alternate Names
FLJ21841; NES; Nestin

Product Datasheets

Citations for Rat Nestin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Conditional inactivation of Pen-2 in the developing neocortex leads to rapid switch of apical progenitors to basal progenitors
    Authors: S Cheng, T Liu, Y Hu, Y Xia, J Hou, C Huang, X Zou, J Liang, Y Stone Shi, Y Zheng, J Lu, G Chen
    J. Neurosci., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Differential expression of nuclear lamin subtypes in the neural cells of the adult rat cerebral cortex
    Authors: Y Takamori, Y Hirahara, T Wakabayash, T Mori, T Koike, Y Kataoka, Y Tamura, S Kurebayash, K Kurokawa, H Yamada
    IBRO Rep, 2018;5(0):99-109.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. Nestin-positive microglia in adult rat cerebral cortex.
    Authors: Takamori Y, Mori T, Wakabayashi T, Nagasaka Y, Matsuzaki T, Yamada H
    Brain Res., 2009;1270(0):10-8.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. Pten deletion in adult neural stem/progenitor cells enhances constitutive neurogenesis.
    Authors: Gregorian C, Nakashima J, Le Belle J, Ohab J, Kim R, Liu A, Smith KB, Groszer M, Garcia AD, Sofroniew MV, Carmichael ST, Kornblum HI, Liu X, Wu H
    J. Neurosci., 2009;29(6):1874-86.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Mechanisms of murine lacrimal gland repair after experimentally induced inflammation.
    Authors: Zoukhri D, Fix A, Alroy J, Kublin CL
    Invest. Ophthalmol. Vis. Sci., 2008;49(10):4399-406.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Vasopressin mediates mitogenic responses to adrenalectomy in the rat anterior pituitary.
    Authors: Subburaju S, Aguilera G
    Endocrinology, 2007;148(7):3102-10.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.
    Authors: De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD
    Biotechnol. Bioeng., 2007;97(6):1617-25.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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