Neuron-specific beta-III Tubulin Antibody (TuJ-1) [NL637]

R&D Systems | Catalog # NL1195V

R&D Systems

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry

Label

NL637 (Excitation = 637 nm, Emission = 658 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # TuJ-1
View all beta-III Tubulin Primary Antibodies »

Product Specifications

Immunogen

Rat brain-derived microtubules

Specificity

Detects neuron-specific beta -III Tubulin in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Neuron-specific beta-III Tubulin Antibody (TuJ-1) [NL637]

Detection of Neuron-specific beta-III Tubulin NL637 by Western Blot

Detection of Neuron-specific beta-III Tubulin NL637 by Western Blot

ACY-738 induced changes in Ki67 and TUJ1 are not observed in ARL13B-KO, cilia-depleted human glioma cells. (A–P) S7 parental cells (left two columns) or ARL13B KO (clone G12) (right two columns) were grown to ~75% confluency and treated with vehicle or 500-nM 738. Cells were fixed after 24 h and immunostained for Ki67 (A–D), TUJ1 (E–H), and ARL13B (I–L). Merged images are displayed M-P. Scale bar in A = 10 µm. (Q) Mean nuclear intensity of Ki67 signal for indicated cell lines and treatments. n = 60–80 cells/group. * p < 0.05, **** p < 0.0001, ANOVA. (R) Mean soma intensity of TUJ1 signal for indicated cell lines and treatments. (S,T) Western blot of S7 (S) and L0 (T) cell lysates 24 h after exposure to vehicle or 500-nM 738. Blots were probed for TUJ1 and beta -actin as loading controls. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33915983), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Neuron-specific beta-III Tubulin Antibody (TuJ-1) [NL637]

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using NL1195V in the following applications:

Formulation, Preparation, and Storage

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: beta-III Tubulin

beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III

Entrez Gene IDs

10381 (Human)

Gene Symbol

TUBB3

Additional beta-III Tubulin Products

Product Documents for Neuron-specific beta-III Tubulin Antibody (TuJ-1) [NL637]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Neuron-specific beta-III Tubulin Antibody (TuJ-1) [NL637]

For research use only

Citations for Neuron-specific beta-III Tubulin Antibody (TuJ-1) [NL637]

Customer Reviews for Neuron-specific beta-III Tubulin Antibody (TuJ-1) [NL637] (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Neuron-specific beta-III Tubulin NL637 Antibody (Clone TuJ-1)
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Prostate cancer cells and Human prostatic cell line PNT1A
    Species: Human
    Verified Customer | Posted 11/01/2021
    Neuron-specific beta-III Tubulin Antibody (TuJ-1) [NL637] NL1195V
  • Neuron-specific beta-III Tubulin NorthernLights™ NL637-conjugated Antibody
    Name: Megan Larson
    Application: Flow Cytometry
    Sample Tested: cells
    Species: Multiple
    Verified Customer | Posted 05/02/2017

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