NG2/MCSP Antibody (LHM 2) - Azide and BSA Free

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688PCP (blue) and a matched isotype control NBP2-27287PCP (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to PerCP complex.

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - A surface stain was performed on A375 Cells with NG2/MCSP (LHM2) antibody NB100-2688 (blue) and a matched isotype control NBP1-43319 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). Image from the standard format of this antibody.

Flow Cytometry: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Flow Cytometry: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - A cell surface stain was performed on SK-MEL-28 cells with NG2/MCSP antibody (LHM 2) NB100-2688AF700 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 700.

Western Blot: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873]

Western Blot: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NG2/MCSP (LHM2) knockout (KO) HeLa cell line. PVDF membrane was probed with 1.0 ug/ml of Mouse Anti-Human NG2/MCSP (LHM2) Polyclonal Antibody (Catalog # NB100-2688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for NG2/MCSP (LHM2) at approximately 300 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions. Image from the standard format of this antibody.

Western Blot: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] -

Differentiation of hASCs towards endothelial cells (ECs) in NFC hydrogel. (A) Differentiation of hASCs in 0.125% NFC hydrogel in endothelial growth medium for 21 days. Scale bars, 100 um (d1) and 50 um (d10, d21). (B) Differentiated cells in 0.125% NFC hydrogel on d7 showing branching of cellular structures (arrows). Scale bars, 25 um. (C) Western blot showing expression of endothelial specific marker CD31 and alpha-smooth muscle actin ( alpha -SMA) in hASCs vs. human umbilical vein endothelial cells (HUVEC) (n = 3, 1 technical replicate). (D) Western blot of pericyte marker NG2 in hASCs and HUVECs. (E) Quantification of NG2 expression (n = 3, 1 technical replicate). * p < 0.05. Ang-1, angiopoietin-1; diff., differentiated cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36289846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NG2/MCSP Antibody (LHM 2) - Azide and BSA Free [NBP2-80873] -

Differentiation of hASCs towards endothelial cells (ECs) in NFC hydrogel. (A) Differentiation of hASCs in 0.125% NFC hydrogel in endothelial growth medium for 21 days. Scale bars, 100 um (d1) and 50 um (d10, d21). (B) Differentiated cells in 0.125% NFC hydrogel on d7 showing branching of cellular structures (arrows). Scale bars, 25 um. (C) Western blot showing expression of endothelial specific marker CD31 and alpha-smooth muscle actin ( alpha -SMA) in hASCs vs. human umbilical vein endothelial cells (HUVEC) (n = 3, 1 technical replicate). (D) Western blot of pericyte marker NG2 in hASCs and HUVECs. (E) Quantification of NG2 expression (n = 3, 1 technical replicate). * p < 0.05. Ang-1, angiopoietin-1; diff., differentiated cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36289846), licensed under a CC-BY license. Not internally tested by Novus Biologicals.