Nitrotyrosine Antibody (39B6) - BSA Free

Novus Biologicals | Catalog # NB110-96877

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Rat, Non-species specific

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 39B6

Format

BSA Free
View all Nitrotyrosine Primary Antibodies »

Product Specifications

Immunogen

3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin

Reactivity Notes

Species Independent. Rat reactivity reported in scientific literature (PMID:32980492)

Specificity

Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Nitrotyrosine Antibody (39B6) - BSA Free

Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877]

Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877]

Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human HEK293 cells showing detection of Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane A: Nitrosylated-HEK293 (15uL). Lane B: HEK293 (15 ug). Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) diluted in 1.5% BSA and TBST for 1 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG: HRP at 1:4000 for 1 hour at RT with shaking. Predicted/Observed Size: Multiple Bands.
Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877]

Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877]

Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.
Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877]

Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877]

Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human A549 cells showing detection of Multiple Bands Nitrotyrosineprotein using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (NB110-96877) at 1:1000 for 2.5 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.
Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877]

Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877]

Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human Recombinant Protein showing detection of Multiple Bands Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane 2: hASYN Monomer (3.84 ug). Lane 3: Nitrosylated hASYN (3.84 ug).. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 2 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.
Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877]

Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877]

Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (39B6) [NB110-96877]

Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (39B6) [NB110-96877]

Nitrotyrosine-Antibody-39B6-Immunohistochemistry-Paraffin-NB110-96877-img0015.jpg
Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877]

Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877]

Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x. With anti-microbial. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications.
Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877]

Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877]

Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: liver tissue. Species: Rat. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green).
Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877]

Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877]

Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.

Applications for Nitrotyrosine Antibody (39B6) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:1400

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Nitrotyrosine

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

Alternate Names

Nitrotyrosine

Additional Nitrotyrosine Products

Product Documents for Nitrotyrosine Antibody (39B6) - BSA Free

Certificate of Analysis

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Product Specific Notices for Nitrotyrosine Antibody (39B6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nitrotyrosine Antibody (39B6) - BSA Free

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Protocols

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