NLRP3/NALP3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77080

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, E. coli

Cited:

Human, Mouse, Bacteria - Escherichia coli

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, SDS-Page

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC, SDS-Page

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NLRP3/NALP3 Primary Antibodies »

Product Specifications

Immunogen

NLRP3/NALP3 Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human NALP3. The immunogen is located within amino acids 120 - 170 of NALP3.

Reactivity Notes

E. coli reactivity reported in scientific literature (PMID: 31551961).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NLRP3/NALP3 Antibody - BSA Free

Western Blot: NLRP3/NALP3 AntibodyBSA Free [NBP1-77080]

Western Blot: NLRP3/NALP3 AntibodyBSA Free [NBP1-77080]

NLRP3-NALP3-Antibody---BSA-Free-Western-Blot-NBP1-77080-img0011.jpg
Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080]

Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080]

Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Staining of NALP3 in K562 cells with NALP3 antibody at 2 ug/mL.
Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080]

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080]

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Analysis of NALP3 in mouse lung tissue using anti-NALP3 antibody. Image from verified customer review.
Western Blot: NLRP3/NALP3 AntibodyBSA Free [NBP1-77080]

Western Blot: NLRP3/NALP3 AntibodyBSA Free [NBP1-77080]

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Analysis of NALP3 in K562 cell lysate with NALP3 antibody at 1 ug/mL (A) in the absence and (B) in the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080]

Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080]

NLRP3-NALP3 Antibody - BSA Free-Immunocytochemistry-Immunofluorescence-NBP1-77080-img0012.jpg
NLRP3/NALP3 Antibody - BSA Free

Immunohistochemistry: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] -

hADSC-Evs suppressed NLRP3 inflammasome activation in ocular surface of experimental mice under desiccating stress. (A) Representative image of immunofluorescent staining of NLRP3 related genes in corneal and conjunctival epithelia of desiccating mice or treated with hADSC-EVs (*conjunctiva; open triangle cornea). (B,C) qRT-PCR showed the mRNA levels of NLRP3 related genes in conjunctiva and cornea (four corneas/conjunctivas were mixed as a sample). Data was shown as mean ± SEM. *p < 0.05.
NLRP3/NALP3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] -

Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - hADSC-Evs suppressed NLRP3 inflammasome activation in ocular surface of experimental mice under desiccating stress. (A) Representative image of immunofluorescent staining of NLRP3 related genes in corneal & conjunctival epithelia of desiccating mice or treated with hADSC-EVs (*conjunctiva; open triangle cornea). (B,C) qRT-PCR showed the mRNA levels of NLRP3 related genes in conjunctiva & cornea (four corneas/conjunctivas were mixed as a sample). Data was shown as mean ± SEM. *p < 0.05. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32884023), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
NLRP3/NALP3 Antibody - BSA Free

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] -

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - HMGB1 promoted the activation of canonical NLRP3 inflammasome in retinal IR injury. The intravitreal injection of exogenous rHMGB1 significantly promoted the mRNA & protein production of NLRP3 (a, c) & ASC (e, g) & activation of caspase-1 (i). Thus, the intravitreal injection of HMGB1 inhibitor, GA, significantly suppressed the production of NLRP3 (b, d) & ASC (f, h) & activation of caspase-1 (j). Data are shown as mean ± SD. *P < 0.05, **P < 0.01 vs sham group Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26224068), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
NLRP3/NALP3 Antibody - BSA Free

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] -

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Co-immunoprecipitation assay: Figure showing interaction of NLRP3, NLRC4 & NAIP with ASC during CFT073 infection in THP-1m. Cytoplasmic extracts of THP-1m infected with or without CFT073, 6 h post stimulation were subjected to IP & WB by various antibodies (ASC, NLRP3, NLRC4, & NAIP) as described in material & methods. Cytoplasmic extract used as input was subjected to check NLRP3, ASC, NLRC4 & NAIP expression as shown in figure. ASC antibody was used to co-immunoprecipitate the complex from cytoplasmic extracts & then immunoblotted to detect NLRP3, NLRC4 & NAIP. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31551961), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
NLRP3/NALP3 Antibody - BSA Free

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] -

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - HMGB1 regulated the activation of NLRP3, rather than the activation of caspase-8, via NF-kappa B pathway. a, b NF-kappa B was activated in ischemic retina at the early stage after reperfusion. c, d Intraocular injection rHMGB1 or GA could promote or suppress the production of phosphor-NF-kappa B p65. e, f Intravitreal injection of NF-kappa B p65 inhibitor, JSH-23, significantly reduced the activation of NLRP3 & decreased the processing of IL-1 beta, g rather than caspase-8. However, intravitreal injection of caspase-8 inhibitor, Z-IETD-fmk, decreased the production of phosphor-NF-kappa B p65, obviously (h). Data are shown as mean ± SD. *P < 0.05, **P < 0.01 vs sham group Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26224068), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for NLRP3/NALP3 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

reported in scientific literature (PMID 31551961)

SDS-Page

reported in scientific literature (PMID 31551961)

Western Blot

1 - 2 ug/mL

Reviewed Applications

Read 2 reviews rated 4 using NBP1-77080 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NLRP3/NALP3

NACHT, LRR and PYD domains-containing protein 3 (NALP3), Nucleotide-Binding Oligomerization Domain, or Leucine Rich Repeat and Pyrin Domain Containing 3 (NLRP3) (human NLRP3-isoform 2 theoretical molecular weight 118kDa) acts as a cytosolic receptor for stress-signals initiated by a wide variety of pathogen-associated molecular patterns (PAMPS) and danger-associated molecular patterns (DAMPs) (1). NLRP3 belongs to the family of NOD-like receptors, a type of pattern-recognition receptor (PRR) which participates in innate immune responses. The exact mechanisms leading to NLRP3 activation are still not fully resolved. Some proposed mechanisms for NLRP3 inflammasome activation include induced ion channel flux as exemplified by potassium efflux, sodium influx and calcium signaling, generation of mitochondrial reactive oxygen species (ROS), and lysosomal destabilization (2). Activation of NLRP3 induces its association with the adaptor protein Apoptosis-associated Speck-like protein containing CARD (ASC) forming a complex that binds to Caspase-1 (1, 2). The inflammasome complex, comprised of NLRP3, ASC and Caspase 1, induces the processing of pro-IL-1beta and pro-IL-18 and release of the mature inflammatory cytokines IL-1beta and IL-18 and pyroptosis (1-3).

NLRP3 is expressed in a variety of cell types such as monocytes, dendritic cells, lymphocytes and epithelial cells (3). Abnormal NLRP3 activation may occur as the result of inherited mutations and is associated with diseases such as hereditary periodic fevers (HPFs) and familial cold autoinflammatory syndrome (FCAS). Additionally, abnormal NLRP3 activation is associated with a variety of diseases such as gout, obesity, atherosclerosis, Alzheimers, multiple sclerosis and type 2 diabetes (1, 3). NLRP3 inflammasome is regulated by several post-translational modifications (e.g., ubiquitination, phosphorylation and sumoylation) as well as by various interacting proteins (e.g., JNK1, FBXO3, TXNIP, MARK4 and PKD) (4).

References

1. Abderrazak, A., Syrovets, T., Couchie, D., El Hadri, K., Friguet, B., Simmet, T., & Rouis, M. (2015). NLRP3 inflammasome: From a danger signal sensor to a regulatory node of oxidative stress and inflammatory diseases. Redox Biology. https://doi.org/10.1016/j.redox.2015.01.008

2. Yang, Y., Wang, H., Kouadir, M., Song, H., & Shi, F. (2019). Recent advances in the mechanisms of NLRP3 inflammasome activation and its inhibitors. Cell Death and Disease. https://doi.org/10.1038/s41419-019-1413-8

3. Zahid, A., Li, B., Kombe, A. J. K., Jin, T., & Tao, J. (2019). Pharmacological inhibitors of the nlrp3 inflammasome. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2019.02538

4. Kelley, N., Jeltema, D., Duan, Y., & He, Y. (2019). The NLRP3 inflammasome: An overview of mechanisms of activation and regulation. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms20133328

Long Name

NLR Family, Pyrin Domain Containing 2

Alternate Names

AGTAVPRL, AII, CIAS1, Cryopyrin, FCAS, FCU, MWS, NACHT, NALP3, PYPAF1

Entrez Gene IDs

114548 (Human); 216799 (Mouse)

Gene Symbol

NLRP3

UniProt

Q96P20 (Human)

Additional NLRP3/NALP3 Products

Product Documents for NLRP3/NALP3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NLRP3/NALP3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NLRP3/NALP3 Antibody - BSA Free

Customer Reviews for NLRP3/NALP3 Antibody - BSA Free (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Name: Max MacPherson
    Application: Immunohistochemistry-Paraffin
    Sample Tested: mouse lung
    Species: Mouse
    Verified Customer | Posted 11/05/2015
    NALP3 Antibody (NBP1-77080) on Mouse Lung
    NLRP3/NALP3 Antibody - BSA Free NBP1-77080
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: corneal epithelium in mouse
    Species: Mouse
    Verified Customer | Posted 04/03/2015

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Protocols

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FAQs for NLRP3/NALP3 Antibody - BSA Free

Showing  1 - 4 of 4 FAQs Showing All

  • Q: Are the NLRP3/NALP3 antibodies validated in Simple Western?

    A: Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.

  • Q: Does NLRP3/NALP3 antibodies come in lyophilized form?

    A: Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.

  • Q: What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?

    A: A sequence of 16 amino acides within the N-terminal of human NLRP3/NALP3.

  • Q: What is the theoretical molecular weight for NLRP3/NALP3 antibodies?

    A: The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.

  • Q: Are the NLRP3/NALP3 antibodies validated in Simple Western?

    A: Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.

  • Q: Does NLRP3/NALP3 antibodies come in lyophilized form?

    A: Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.

  • Q: What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?

    A: A sequence of 16 amino acides within the N-terminal of human NLRP3/NALP3.

  • Q: What is the theoretical molecular weight for NLRP3/NALP3 antibodies?

    A: The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.

  • Q: Are the NLRP3/NALP3 antibodies validated in Simple Western?

    A: Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.

  • Q: Does NLRP3/NALP3 antibodies come in lyophilized form?

    A: Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.

  • Q: What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?

    A: A sequence of 16 amino acides within the N-terminal of human NLRP3/NALP3.

  • Q: What is the theoretical molecular weight for NLRP3/NALP3 antibodies?

    A: The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.

  • Q: Are the NLRP3/NALP3 antibodies validated in Simple Western?

    A: Yes, we offer a NLRP3/NALP3 antibody that have been tested in Simple Western: NBP2-03948.

  • Q: Does NLRP3/NALP3 antibodies come in lyophilized form?

    A: Yes, we carry 3 NLRP2 antibodies in lyophilized format: AF6789, AF7010, MAB6789, MAB7578.

  • Q: What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?

    A: A sequence of 16 amino acides within the N-terminal of human NLRP3/NALP3.

  • Q: What is the theoretical molecular weight for NLRP3/NALP3 antibodies?

    A: The TMW of NLRP3/NALP3 antibodies is approximately 118 kDa.

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