Notch-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24771

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Bovine, Canine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Notch-1 Primary Antibodies »

Product Specifications

Immunogen

Within the range of amino acids 1755-1780 of human Notch1 were used as the immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Notch-1 Antibody - BSA Free

Western Blot: Notch-1 Antibody [NBP2-24771]

Western Blot: Notch-1 Antibody [NBP2-24771]

Western Blot: Notch-1 Antibody [NBP2-24771] - Analysis of Notch1 in HEK293 cells transfected with full length Notch 1 A) untreated and B) treated with 5 mM EDTA for 15 minutes, using this antibody at 3 ug/mL. The EDTA treatment increases the detected level of Notch1-cv.

Applications for Notch-1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

3 - 5 ug/mL

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 0.2% Gelatin

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Notch-1

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane which then translocates to the nucleus to activate transcription of downstream genes.

Alternate Names

Notch1, TAN1

Gene Symbol

NOTCH1

UniProt

P46531

Additional Notch-1 Products

Product Documents for Notch-1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Notch-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Notch-1 Antibody - BSA Free

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  • Q: Hello, does this product (NB300-251) only recognize the cleavage Notch1?

    A: This antibody with recognize full length Notch1 if it is in the mix, as well as the cleaved product.

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