NPY5R Antibody

Novus Biologicals | Catalog # NB100-1538

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Hamster

Cited:

Human, Rat

Predicted:

Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NPY5R Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GLSNKENRLEEN corresponding to internal region according to NP_006165.1.

Reactivity Notes

Rat reactivity reported in (PMID: 23179670).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NPY5R Antibody

Western Blot: NPY5R Antibody [NB100-1538]

Western Blot: NPY5R Antibody [NB100-1538]

Western Blot: NPY5R Antibody [NB100-1538] - Staining of Human Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NPY5R Antibody [NB100-1538]

Immunohistochemistry-Paraffin: NPY5R Antibody [NB100-1538]

Immunohistochemistry-Paraffin: NPY5R Antibody [NB100-1538] - Staining of Human Intestine. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for NPY5R Antibody

Application
Recommended Usage

Immunohistochemistry

4 - 6 ug/mL

Immunohistochemistry-Paraffin

4 - 6 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/mL
Application Notes
This NPY5R Antibody is validated for IP from a verified customer review. WB: Approx. 50 kDa band observed in human brain, breast and duodenum lysates (calculated MW of 50.7 kDa band according to NP_006165.1). IHC-P: Human intestine shows staining of ganglion cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NPY5R

NPY5R is a receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders.

Long Name

Neuropeptide Y Receptor Y5

Alternate Names

neuropeptide Y receptor type 5, neuropeptide Y receptor Y5, NPYR5NPY5-R, NPY-Y5 receptor, NPYY5-R, Y5 receptor

Entrez Gene IDs

4889 (Human)

Gene Symbol

NPY5R

UniProt

Q15761 (Human)

Additional NPY5R Products

Product Documents for NPY5R Antibody

Certificate of Analysis

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Product Specific Notices for NPY5R Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for NPY5R Antibody

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  • Q: Why dose your Y5R antibodies produces 2 different molecular weight products in human tissues or human cell lysates? NB100-1538 ab probes to 50KDa protein while the rabbit Y5R ab probes to 40-42KDa protein?

    A: NPY5R is a glycosylated protein. This can cause it to run at varying molecular weights depending on the cell line and Western blot conditions. The 48 kDa band represents the protein in it's unglycosylated form whereas the higher molecular weights above 50 kDa are from blots generated in conditions when the protein is in its glycosylated form.

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