O-GlcNAc Antibody (RL2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80892

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Drosophila, Fish, Hamster, Primate, Virus, Xenopus

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Dot Blot, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # RL2

Format

Azide and BSA Free
View all O-GlcNAc Primary Antibodies »

Product Specifications

Immunogen

Pore complex-lamina fraction purified from rat liver nuclear envelopes.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 26004176). Xenopus reactivity reported in scientific literature (PMID: 17329255). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic and Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for O-GlcNAc Antibody (RL2) - Azide and BSA Free

Western Blot: O-GlcNAc Antibody (RL2)Azide and BSA Free [NBP2-80892]

Western Blot: O-GlcNAc Antibody (RL2)Azide and BSA Free [NBP2-80892]

Western Blot: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892] - Analysis of mouse cortical brain lysates using O-Linked N-Acetylglucosamine Monoclonal Antibody. Blots containing cortical extracts from 4 individual C57BL/6 mice (Lanes 1-4) were blocked with 5% milk in TBST, and probed with MA1-072 at 1:1000, followed by a fluorophore-conjugated goat anti-mouse IgG secondary antibody. Data courtesy of the Innovators Program. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Immunocytochemistry/ Immunofluorescence: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Immunocytochemistry/Immunofluorescence: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-O-GlcNAc (RL2) at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Immunohistochemistry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Immunohistochemistry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892] - Analysis of a FFPE tissue section of the mouse colon using 1:200 dilution of O-GlcNAc [RL2] antibody (NB300-524). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. Image from the standard format of
Flow (Intracellular): O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Flow (Intracellular): O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Flow (Intracellular): O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892] - An intracellular stain was performed on RH30 cells with O-GlcNAc [RL2] Antibody NB300-524AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.
Immunocytochemistry/ Immunofluorescence: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Immunocytochemistry/ Immunofluorescence: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Immunocytochemistry/Immunofluorescence: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-O-GlcNAc (RL2) at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892] - An intracellular stain was performed on HeLa cells with O-GlcNAc Antibody [RL2] Antibody NB300-524AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.
Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892] - An intracellular stain was performed on Jurkat cells with O-GlcNAc antibody (RL2) NB300-524PE (blue) and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.
Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892] - An intracellular stain was performed on Neuro2a cells with O-GlcNAc Antibody [RL2] NB300-524AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.
Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892] - An intracellular stain was performed on SK-MEL-28 cells with O-GlcNAc antibody (RL2) NB300-524AF647 (blue) and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.
Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892] - An intracellular stain was performed on U-937 cells with O-GlcNAc antibody (RL2) NB300-524AF647 (blue) and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.
Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892]

Flow Cytometry: O-GlcNAc Antibody (RL2) - Azide and BSA Free [NBP2-80892] - Analysis using Alexa Fluor (R) 647 conjugate of NB300-524. An intracellular stain was performed on Jurkat cells with O-GlcNAc antibody (RL2) NB300-524 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 ug of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.

Applications for O-GlcNAc Antibody (RL2) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

1:10 - 1:500. Use reported in scientific literature (PMID 20404350)

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Dot Blot

1:800

ELISA

1:100 - 1:2000,. Use reported in scientific literature (PMID 12029848)

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10 - 1:500

Western Blot

1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: O-GlcNAc

Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.

Alternate Names

GlcNAc, O-linked N-acetylglucosamine

Additional O-GlcNAc Products

Product Documents for O-GlcNAc Antibody (RL2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for O-GlcNAc Antibody (RL2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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