Optineurin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84682

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Optineurin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LGIVSELQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKG

Reactivity Notes

Reactivity reported in mouse retina from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Optineurin Antibody - BSA Free

Western Blot: Optineurin Antibody [NBP1-84682]

Western Blot: Optineurin Antibody [NBP1-84682]

Western Blot: Optineurin Antibody [NBP1-84682] - Analysis using Anti-OPTN antibody NBP1-84682 (A) shows similar pattern to independent antibody NBP1-84683 (B).
Immunocytochemistry/ Immunofluorescence: Optineurin Antibody [NBP1-84682]

Immunocytochemistry/ Immunofluorescence: Optineurin Antibody [NBP1-84682]

Immunocytochemistry/Immunofluorescence: Optineurin Antibody [NBP1-84682] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining shown in green.
Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682]

Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682]

Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.
Western Blot: Optineurin Antibody [NBP1-84682]

Western Blot: Optineurin Antibody [NBP1-84682]

Western Blot: Optineurin Antibody [NBP1-84682] - Effect of mitochondrial fission protein, Drp1 (FL) and mutant Tau on Optineurin expression in N2a (M) cells. Lanes: M. Ladder M. Ladder 1. N2a cells 2. N2a cells + mutant Tau 3. N2a cells + mutant tau + Drp1 full length + mutant Tau. This image was submitted via customer review.
Immunohistochemistry-Frozen: Optineurin Antibody [NBP1-84682]

Immunohistochemistry-Frozen: Optineurin Antibody [NBP1-84682]

Immunohistochemistry-Frozen: Optineurin Antibody [NBP1-84682] - Image and review of Optineurin antibody on mouse retina (frozen sections) by Celia McKee, Duke University. 20X and 100X.
Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682]

Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682]

Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682]

Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682]

Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.
Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682]

Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682]

Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.
Optineurin Antibody - BSA Free Western Blot: Optineurin Antibody - BSA Free [NBP1-84682]

Western Blot: Optineurin Antibody - BSA Free [NBP1-84682]

Analysis in human cell lines A-431 and PC-3 using Anti-OPTN antibody. Corresponding OPTN RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.

Applications for Optineurin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Frozen

Validated from a verified customer review.

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC-Paraffin HIER pH6 retrieval is recommended.

Reviewed Applications

Read 2 reviews rated 5 using NBP1-84682 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Optineurin

Optineurin encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Alternate Names

ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, OPTN

Gene Symbol

OPTN

Additional Optineurin Products

Product Documents for Optineurin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Optineurin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy

Citations for Optineurin Antibody - BSA Free

Customer Reviews for Optineurin Antibody - BSA Free (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Optineurin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Protein Lysate
    Species: Mouse
    Verified Customer | Posted 02/20/2018
    Effect of mitochondrial fission protein, Drp1 (FL) and mutant Tau on Optineurin expression in N2a (M) cells. Lanes: M. Ladder M. Ladder 1. N2a cells 2. N2a cells + mutant Tau 3. N2a cells + mutant tau + Drp1 full length + mutant Tau
    Optineurin Antibody - BSA Free NBP1-84682
  • Name: Bryan Tinsley
    Application: Immunohistochemistry-Frozen
    Sample Tested:
    Species: Mouse
    Verified Customer | Posted 08/16/2014
    Mouse retina 20X using Optineurin antibody, Image from Celia McKee, Duke
    Optineurin Antibody - BSA Free NBP1-84682

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Protocols

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