p23/PTGES3 Antibody (JJ3)

Flow Cytometry: p23/PTGES3 Antibody (JJ3) [NB300-576]

Flow Cytometry: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of 3T3 cells compared to an isotype control (blue).

Immunocytochemistry/ Immunofluorescence: p23/PTGES3 Antibody (JJ3) [NB300-576]

Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (JJ3) [NB300-576] - p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing p23 at a dilution of 1:500 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/ Immunofluorescence: p23/PTGES3 Antibody (JJ3) [NB300-576]

Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (JJ3) [NB300-576] - p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing p23 at a dilution of 1:500 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JJ3) [NB300-576]

Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JJ3) [NB300-576] - Cancer biopsies of deparaffinized Human breast carcinoma tissue.

Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JJ3) [NB300-576]

Immunohistochemistry-Paraffin: p23/PTGES3 Antibody (JJ3) [NB300-576] - Normal biopsies of deparaffinized Human tonsil tissue.

Flow Cytometry: p23/PTGES3 Antibody (JJ3) [NB300-576]

Flow Cytometry: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of Jurkat cells compared to an isotype control (blue).

Flow Cytometry: p23/PTGES3 Antibody (JJ3) [NB300-576]

Flow Cytometry: p23/PTGES3 Antibody (JJ3) [NB300-576] - Analysis of Hela cells compared to an isotype control (blue).

Western Blot: p23/PTGES3 Antibody (JJ3) [NB300-576] -

P23 and KIF15 expression in untreated and LPS-induced macrophages. LPS induction of RAW 264.7 macrophages (10 ng/ml, 2, 6 and 24 h) (n = 4). (A) QPCR analysis of mRNA levels as indicated, normalized to Ndufv1 mRNA (n = 4). (B) Inhibition of transcription in untreated cells and after 2 and 6 h LPS induction as indicated (n = 3). Kif15 and Actb mRNA were monitored by qPCR, normalized to exogenously added Luc mRNA. (C) Representative Western blot with specific antibodies as indicated, KIF15 was normalized to VINCULIN and P23 to GAPDH. (D) Upper panel: Schematic representation of the experimental design. Lower panel: RAW 264.7 cell migration in response to LPS (n = 3). Statistical analysis was performed with one-way ANOVA, significance levels defined as ** = p < 0.01 and *** = p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34179071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: p23/PTGES3 Antibody (JJ3) [NB300-576] -

P23 or KIF15 depletion enhances untreated macrophage migration. (A) Schematic representation of the experimental design. RAW 264.7 cells were transfected with control (scr), P23 (#749, #1672) or Kif15 (#544, #2481) specific siRNAs and treated as depicted (n = 3). (B) Representative Western blot with antibodies as indicated. KIF15 was normalized to VINCULIN and P23 to GAPDH. (C) Left panel: Analysis of migrating cells by microscopy. Right panel: Quantification of migrating cells (n = 3). (D) Morphology of migrated cells (DAPI staining and bright-field microscopy). Statistical analysis was performed with one-way ANOVA, significance levels defined as *** = p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34179071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: p23/PTGES3 Antibody (JJ3) [NB300-576] -

P23 depletion enhances phagocytosis by LPS-induced RAW 264.7 macrophages. (A) Schematic representation of the experimental design. (B) Cells were transfected with control (scr) or P23-specific siRNAs (#749) and stimulated with LPS (100 ng/ml) (n = 3). Representative Western blot of cytosolic RAW 264.7 cell extracts with antibodies as indicated, P23 was normalized to GAPDH. (C) Following siRNA transfection and LPS treatment, cells were incubated with Fluoresbrite® carboxylate labeled latex beads and analyzed by IF microscopy, as indicated. (D) The phagocytosis index was calculated as 103 beads ingested by 100 cells. Statistical analysis was performed with one-way ANOVA, significance levels defined as *** = p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34179071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: p23/PTGES3 Antibody (JJ3) [NB300-576] -

P23 depletion from untreated macrophages results in Kif15 mRNA and protein decline. (A) RAW 264.7 cells were transfected with control (scr) or P23 siRNAs (#749, #1672) (n = 3). Representative Western blot with antibodies as indicated. KIF15 was normalized to VINCULIN and P23 to GAPDH. (B) Analysis of mRNA levels by qPCR, normalized to Ndufv1 mRNA (n = 3). (C) Inhibition of transcription in untreated cells, as indicated (n = 3). Kif15 and Actb mRNAs were monitored by qPCR, normalized to exogenously added Luc mRNA. Statistical analysis was performed with one-way ANOVA, significance levels defined as ** = p < 0.01 and *** = p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34179071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.